Influence of vitamin K2 on lipid precursors of inflammation and fatty acids pathway activities in HepG2 cells

Vitamin K2 (VK2) is one of the two types of vitamin K present most in the human diet. VK2 seems to have a beneficial effect on inflammation related to type 2 diabetes mellitus. The aim of this study was to evaluate the influence of VK2 on lipid precursors of inflammation in lipid-overloaded human li...

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Autores principales:	Adrian Kołakowski, Piotr Franciszek Kurzyna, Wiktor Bzdęga, Hubert Żywno, Ewa Harasim-Symbor, Adrian Chabowski, Karolina Konstantynowicz-Nowicka
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Vitamin K2 Inflammation Hepatocytes Triacylglycerols Arachidonic acid Cytology QH573-671
Acceso en línea:	https://doaj.org/article/5278401620b3444eb99cedfe819904f5
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spelling	oai:doaj.org-article:5278401620b3444eb99cedfe819904f52021-11-26T04:23:50ZInfluence of vitamin K2 on lipid precursors of inflammation and fatty acids pathway activities in HepG2 cells0171-933510.1016/j.ejcb.2021.151188https://doaj.org/article/5278401620b3444eb99cedfe819904f52021-09-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S017193352100039Xhttps://doaj.org/toc/0171-9335Vitamin K2 (VK2) is one of the two types of vitamin K present most in the human diet. VK2 seems to have a beneficial effect on inflammation related to type 2 diabetes mellitus. The aim of this study was to evaluate the influence of VK2 on lipid precursors of inflammation in lipid-overloaded human liver hepatocellular carcinoma cells. Cells were incubated with VK2 and/or palmitic acid (PA). The concentrations of lipid fractions and their fatty acid compositions were measured by gas-liquid chromatography. The expression of proteins involved in the inflammatory process was detected using western blotting. The concentration of triacylglycerols (TAGs), activities of the n-3 pathway in TAGs, and lipooxygenase 15 expression were significantly elevated in cells incubated with PA and VK2. In the same group, a marked elevation in diacylglycerol (DAG) 20:4 was observed. VK2 supplementation lowered the expression of tumour necrosis factor-alpha and interleukin-6 compared to that in the PA group. The data indicate that VK2 redirects fatty acid metabolism into the deposition of a safe TAG fraction by increasing the concentration of anti-inflammatory n-3 polyunsaturated fatty acids in this fraction. Moreover, VK2 stimulates the synthesis of anti-inflammatory factors and has anti-inflammatory effects by reducing DAG 20:4.Adrian KołakowskiPiotr Franciszek KurzynaWiktor BzdęgaHubert ŻywnoEwa Harasim-SymborAdrian ChabowskiKarolina Konstantynowicz-NowickaElsevierarticleVitamin K2InflammationHepatocytesTriacylglycerolsArachidonic acidCytologyQH573-671ENEuropean Journal of Cell Biology, Vol 100, Iss 7, Pp 151188- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Vitamin K2 Inflammation Hepatocytes Triacylglycerols Arachidonic acid Cytology QH573-671
spellingShingle	Vitamin K2 Inflammation Hepatocytes Triacylglycerols Arachidonic acid Cytology QH573-671 Adrian Kołakowski Piotr Franciszek Kurzyna Wiktor Bzdęga Hubert Żywno Ewa Harasim-Symbor Adrian Chabowski Karolina Konstantynowicz-Nowicka Influence of vitamin K2 on lipid precursors of inflammation and fatty acids pathway activities in HepG2 cells
description	Vitamin K2 (VK2) is one of the two types of vitamin K present most in the human diet. VK2 seems to have a beneficial effect on inflammation related to type 2 diabetes mellitus. The aim of this study was to evaluate the influence of VK2 on lipid precursors of inflammation in lipid-overloaded human liver hepatocellular carcinoma cells. Cells were incubated with VK2 and/or palmitic acid (PA). The concentrations of lipid fractions and their fatty acid compositions were measured by gas-liquid chromatography. The expression of proteins involved in the inflammatory process was detected using western blotting. The concentration of triacylglycerols (TAGs), activities of the n-3 pathway in TAGs, and lipooxygenase 15 expression were significantly elevated in cells incubated with PA and VK2. In the same group, a marked elevation in diacylglycerol (DAG) 20:4 was observed. VK2 supplementation lowered the expression of tumour necrosis factor-alpha and interleukin-6 compared to that in the PA group. The data indicate that VK2 redirects fatty acid metabolism into the deposition of a safe TAG fraction by increasing the concentration of anti-inflammatory n-3 polyunsaturated fatty acids in this fraction. Moreover, VK2 stimulates the synthesis of anti-inflammatory factors and has anti-inflammatory effects by reducing DAG 20:4.
format	article
author	Adrian Kołakowski Piotr Franciszek Kurzyna Wiktor Bzdęga Hubert Żywno Ewa Harasim-Symbor Adrian Chabowski Karolina Konstantynowicz-Nowicka
author_facet	Adrian Kołakowski Piotr Franciszek Kurzyna Wiktor Bzdęga Hubert Żywno Ewa Harasim-Symbor Adrian Chabowski Karolina Konstantynowicz-Nowicka
author_sort	Adrian Kołakowski
title	Influence of vitamin K2 on lipid precursors of inflammation and fatty acids pathway activities in HepG2 cells
title_short	Influence of vitamin K2 on lipid precursors of inflammation and fatty acids pathway activities in HepG2 cells
title_full	Influence of vitamin K2 on lipid precursors of inflammation and fatty acids pathway activities in HepG2 cells
title_fullStr	Influence of vitamin K2 on lipid precursors of inflammation and fatty acids pathway activities in HepG2 cells
title_full_unstemmed	Influence of vitamin K2 on lipid precursors of inflammation and fatty acids pathway activities in HepG2 cells
title_sort	influence of vitamin k2 on lipid precursors of inflammation and fatty acids pathway activities in hepg2 cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5278401620b3444eb99cedfe819904f5
work_keys_str_mv	AT adriankołakowski influenceofvitamink2onlipidprecursorsofinflammationandfattyacidspathwayactivitiesinhepg2cells AT piotrfranciszekkurzyna influenceofvitamink2onlipidprecursorsofinflammationandfattyacidspathwayactivitiesinhepg2cells AT wiktorbzdega influenceofvitamink2onlipidprecursorsofinflammationandfattyacidspathwayactivitiesinhepg2cells AT hubertzywno influenceofvitamink2onlipidprecursorsofinflammationandfattyacidspathwayactivitiesinhepg2cells AT ewaharasimsymbor influenceofvitamink2onlipidprecursorsofinflammationandfattyacidspathwayactivitiesinhepg2cells AT adrianchabowski influenceofvitamink2onlipidprecursorsofinflammationandfattyacidspathwayactivitiesinhepg2cells AT karolinakonstantynowicznowicka influenceofvitamink2onlipidprecursorsofinflammationandfattyacidspathwayactivitiesinhepg2cells
_version_	1718409900377243648

Influence of vitamin K2 on lipid precursors of inflammation and fatty acids pathway activities in HepG2 cells

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