Involvement of a minimal actin-binding region of Spiroplasma citri phosphoglycerate kinase in spiroplasma transmission by its leafhopper vector.

<h4>Background</h4>Spiroplasma citri is a wall-less bacterium that colonizes phloem vessels of a large number of host plants. Leafhopper vectors transmit S. citri in a propagative and circulative manner, involving colonization and multiplication of bacteria in various insect organs. Prev...

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Autores principales:	Fabien Labroussaa, Marie-Pierre Dubrana, Nathalie Arricau-Bouvery, Laure Béven, Colette Saillard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/527c2d846288485dbbe8f5d1b71214e1
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spelling	oai:doaj.org-article:527c2d846288485dbbe8f5d1b71214e12021-11-18T06:58:28ZInvolvement of a minimal actin-binding region of Spiroplasma citri phosphoglycerate kinase in spiroplasma transmission by its leafhopper vector.1932-620310.1371/journal.pone.0017357https://doaj.org/article/527c2d846288485dbbe8f5d1b71214e12011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21364953/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Spiroplasma citri is a wall-less bacterium that colonizes phloem vessels of a large number of host plants. Leafhopper vectors transmit S. citri in a propagative and circulative manner, involving colonization and multiplication of bacteria in various insect organs. Previously we reported that phosphoglycerate kinase (PGK), the well-known glycolytic enzyme, bound to leafhopper actin and was unexpectedly implicated in the internalization process of S. citri into Circulifer haematoceps cells.<h4>Methodology/principal findings</h4>In an attempt to identify the actin-interacting regions of PGK, several overlapping PGK truncations were generated. Binding assays, using the truncations as probes on insect protein blots, revealed that the actin-binding region of PGK was located on the truncated peptide designated PGK-FL5 containing amino acids 49-154. To investigate the role of PGK-FL5-actin interaction, competitive spiroplasma attachment and internalization assays, in which His(6)-tagged PGK-FL5 was added to Ciha-1 cells prior to infection with S. citri, were performed. No effect on the efficiency of attachment of S. citri to leafhopper cells was observed while internalization was drastically reduced. The in vivo effect of PGK-FL5 was confirmed by competitive experimental transmission assays as injection of PGK-FL5 into S. citri infected leafhoppers significantly affected spiroplasmal transmission.<h4>Conclusion</h4>These results suggest that S. citri transmission by its insect vector is correlated to PGK ability to bind actin.Fabien LabroussaaMarie-Pierre DubranaNathalie Arricau-BouveryLaure BévenColette SaillardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e17357 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fabien Labroussaa Marie-Pierre Dubrana Nathalie Arricau-Bouvery Laure Béven Colette Saillard Involvement of a minimal actin-binding region of Spiroplasma citri phosphoglycerate kinase in spiroplasma transmission by its leafhopper vector.
description	<h4>Background</h4>Spiroplasma citri is a wall-less bacterium that colonizes phloem vessels of a large number of host plants. Leafhopper vectors transmit S. citri in a propagative and circulative manner, involving colonization and multiplication of bacteria in various insect organs. Previously we reported that phosphoglycerate kinase (PGK), the well-known glycolytic enzyme, bound to leafhopper actin and was unexpectedly implicated in the internalization process of S. citri into Circulifer haematoceps cells.<h4>Methodology/principal findings</h4>In an attempt to identify the actin-interacting regions of PGK, several overlapping PGK truncations were generated. Binding assays, using the truncations as probes on insect protein blots, revealed that the actin-binding region of PGK was located on the truncated peptide designated PGK-FL5 containing amino acids 49-154. To investigate the role of PGK-FL5-actin interaction, competitive spiroplasma attachment and internalization assays, in which His(6)-tagged PGK-FL5 was added to Ciha-1 cells prior to infection with S. citri, were performed. No effect on the efficiency of attachment of S. citri to leafhopper cells was observed while internalization was drastically reduced. The in vivo effect of PGK-FL5 was confirmed by competitive experimental transmission assays as injection of PGK-FL5 into S. citri infected leafhoppers significantly affected spiroplasmal transmission.<h4>Conclusion</h4>These results suggest that S. citri transmission by its insect vector is correlated to PGK ability to bind actin.
format	article
author	Fabien Labroussaa Marie-Pierre Dubrana Nathalie Arricau-Bouvery Laure Béven Colette Saillard
author_facet	Fabien Labroussaa Marie-Pierre Dubrana Nathalie Arricau-Bouvery Laure Béven Colette Saillard
author_sort	Fabien Labroussaa
title	Involvement of a minimal actin-binding region of Spiroplasma citri phosphoglycerate kinase in spiroplasma transmission by its leafhopper vector.
title_short	Involvement of a minimal actin-binding region of Spiroplasma citri phosphoglycerate kinase in spiroplasma transmission by its leafhopper vector.
title_full	Involvement of a minimal actin-binding region of Spiroplasma citri phosphoglycerate kinase in spiroplasma transmission by its leafhopper vector.
title_fullStr	Involvement of a minimal actin-binding region of Spiroplasma citri phosphoglycerate kinase in spiroplasma transmission by its leafhopper vector.
title_full_unstemmed	Involvement of a minimal actin-binding region of Spiroplasma citri phosphoglycerate kinase in spiroplasma transmission by its leafhopper vector.
title_sort	involvement of a minimal actin-binding region of spiroplasma citri phosphoglycerate kinase in spiroplasma transmission by its leafhopper vector.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/527c2d846288485dbbe8f5d1b71214e1
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Involvement of a minimal actin-binding region of Spiroplasma citri phosphoglycerate kinase in spiroplasma transmission by its leafhopper vector.

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