The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus.

The vast majority of viruses consist of a nucleic acid surrounded by a protective icosahedral protein shell called the capsid. During viral infection of a host cell, the timing and efficiency of the assembly process is important for ensuring the production of infectious new progeny virus particles....

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Autores principales:	Sam R Hill, Reidun Twarock, Eric C Dykeman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/52807203e35a4339a203ffa68379a6ab
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spelling	oai:doaj.org-article:52807203e35a4339a203ffa68379a6ab2021-12-02T19:58:01ZThe impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus.1553-734X1553-735810.1371/journal.pcbi.1009306https://doaj.org/article/52807203e35a4339a203ffa68379a6ab2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pcbi.1009306https://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358The vast majority of viruses consist of a nucleic acid surrounded by a protective icosahedral protein shell called the capsid. During viral infection of a host cell, the timing and efficiency of the assembly process is important for ensuring the production of infectious new progeny virus particles. In the class of single-stranded RNA (ssRNA) viruses, the assembly of the capsid takes place in tandem with packaging of the ssRNA genome in a highly cooperative co-assembly process. In simple ssRNA viruses such as the bacteriophage MS2 and small RNA plant viruses such as STNV, this cooperative process results from multiple interactions between the protein shell and sites in the RNA genome which have been termed packaging signals. Using a stochastic assembly algorithm which includes cooperative interactions between the protein shell and packaging signals in the RNA genome, we demonstrate that highly efficient assembly of STNV capsids arises from a set of simple local rules. Altering the local assembly rules results in different nucleation scenarios with varying assembly efficiencies, which in some cases depend strongly on interactions with RNA packaging signals. Our results provide a potential simple explanation based on local assembly rules for the ability of some ssRNA viruses to spontaneously assemble around charged polymers and other non-viral RNAs in vitro.Sam R HillReidun TwarockEric C DykemanPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 17, Iss 8, p e1009306 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Sam R Hill Reidun Twarock Eric C Dykeman The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus.
description	The vast majority of viruses consist of a nucleic acid surrounded by a protective icosahedral protein shell called the capsid. During viral infection of a host cell, the timing and efficiency of the assembly process is important for ensuring the production of infectious new progeny virus particles. In the class of single-stranded RNA (ssRNA) viruses, the assembly of the capsid takes place in tandem with packaging of the ssRNA genome in a highly cooperative co-assembly process. In simple ssRNA viruses such as the bacteriophage MS2 and small RNA plant viruses such as STNV, this cooperative process results from multiple interactions between the protein shell and sites in the RNA genome which have been termed packaging signals. Using a stochastic assembly algorithm which includes cooperative interactions between the protein shell and packaging signals in the RNA genome, we demonstrate that highly efficient assembly of STNV capsids arises from a set of simple local rules. Altering the local assembly rules results in different nucleation scenarios with varying assembly efficiencies, which in some cases depend strongly on interactions with RNA packaging signals. Our results provide a potential simple explanation based on local assembly rules for the ability of some ssRNA viruses to spontaneously assemble around charged polymers and other non-viral RNAs in vitro.
format	article
author	Sam R Hill Reidun Twarock Eric C Dykeman
author_facet	Sam R Hill Reidun Twarock Eric C Dykeman
author_sort	Sam R Hill
title	The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus.
title_short	The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus.
title_full	The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus.
title_fullStr	The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus.
title_full_unstemmed	The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus.
title_sort	impact of local assembly rules on rna packaging in a t = 1 satellite plant virus.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/52807203e35a4339a203ffa68379a6ab
work_keys_str_mv	AT samrhill theimpactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus AT reiduntwarock theimpactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus AT ericcdykeman theimpactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus AT samrhill impactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus AT reiduntwarock impactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus AT ericcdykeman impactoflocalassemblyrulesonrnapackaginginat1satelliteplantvirus
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The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus.

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