Presence and analysis of plasmids in human and animal associated arcobacter species.

In this study, we report the screening of four Arcobacter species for the presence of small and large plasmids. Plasmids were present in 9.9% of the 273 examined strains. One Arcobacter cryaerophilus and four Arcobacter butzleri plasmids were selected for further sequencing. The size of three small...

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Autores principales:	Laid Douidah, Lieven De Zutter, Filip Van Nieuwerburgh, Dieter Deforce, Hanne Ingmer, Olivier Vandenberg, Anne-Marie Van den Abeele, Kurt Houf
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/528fc7aa79db47dab45692045f02ca0b
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spelling	oai:doaj.org-article:528fc7aa79db47dab45692045f02ca0b2021-11-18T08:37:17ZPresence and analysis of plasmids in human and animal associated arcobacter species.1932-620310.1371/journal.pone.0085487https://doaj.org/article/528fc7aa79db47dab45692045f02ca0b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24465575/?tool=EBIhttps://doaj.org/toc/1932-6203In this study, we report the screening of four Arcobacter species for the presence of small and large plasmids. Plasmids were present in 9.9% of the 273 examined strains. One Arcobacter cryaerophilus and four Arcobacter butzleri plasmids were selected for further sequencing. The size of three small plasmids isolated from A. butzleri and the one from A. cryaerophilus strains ranged between 4.8 and 5.1 kb, and the size of the large plasmid, isolated from A. butzleri, was 27.4 kbp. The G+C content of all plasmids ranged between 25.4% and 26.2%. A total of 95% of the large plasmid sequence represents coding information, which contrasts to the 20 to 30% for the small plasmids. Some of the open reading frames showed a high homology to putative conserved domains found in other related organisms, such as replication, mobilization and genes involved in type IV secretion system. The large plasmid carried 35 coding sequences, including seven genes in a contiguous region of 11.6 kbp that encodes an orthologous type IV secretion system found in the Wolinella succinogenes genome, Helicobacter pylori and Campylobacter jejuni plasmids, which makes this plasmid interesting for further exploration.Laid DouidahLieven De ZutterFilip Van NieuwerburghDieter DeforceHanne IngmerOlivier VandenbergAnne-Marie Van den AbeeleKurt HoufPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e85487 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laid Douidah Lieven De Zutter Filip Van Nieuwerburgh Dieter Deforce Hanne Ingmer Olivier Vandenberg Anne-Marie Van den Abeele Kurt Houf Presence and analysis of plasmids in human and animal associated arcobacter species.
description	In this study, we report the screening of four Arcobacter species for the presence of small and large plasmids. Plasmids were present in 9.9% of the 273 examined strains. One Arcobacter cryaerophilus and four Arcobacter butzleri plasmids were selected for further sequencing. The size of three small plasmids isolated from A. butzleri and the one from A. cryaerophilus strains ranged between 4.8 and 5.1 kb, and the size of the large plasmid, isolated from A. butzleri, was 27.4 kbp. The G+C content of all plasmids ranged between 25.4% and 26.2%. A total of 95% of the large plasmid sequence represents coding information, which contrasts to the 20 to 30% for the small plasmids. Some of the open reading frames showed a high homology to putative conserved domains found in other related organisms, such as replication, mobilization and genes involved in type IV secretion system. The large plasmid carried 35 coding sequences, including seven genes in a contiguous region of 11.6 kbp that encodes an orthologous type IV secretion system found in the Wolinella succinogenes genome, Helicobacter pylori and Campylobacter jejuni plasmids, which makes this plasmid interesting for further exploration.
format	article
author	Laid Douidah Lieven De Zutter Filip Van Nieuwerburgh Dieter Deforce Hanne Ingmer Olivier Vandenberg Anne-Marie Van den Abeele Kurt Houf
author_facet	Laid Douidah Lieven De Zutter Filip Van Nieuwerburgh Dieter Deforce Hanne Ingmer Olivier Vandenberg Anne-Marie Van den Abeele Kurt Houf
author_sort	Laid Douidah
title	Presence and analysis of plasmids in human and animal associated arcobacter species.
title_short	Presence and analysis of plasmids in human and animal associated arcobacter species.
title_full	Presence and analysis of plasmids in human and animal associated arcobacter species.
title_fullStr	Presence and analysis of plasmids in human and animal associated arcobacter species.
title_full_unstemmed	Presence and analysis of plasmids in human and animal associated arcobacter species.
title_sort	presence and analysis of plasmids in human and animal associated arcobacter species.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/528fc7aa79db47dab45692045f02ca0b
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Presence and analysis of plasmids in human and animal associated arcobacter species.

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