An Asian Origin of Virulent <named-content content-type="genus-species">Aeromonas hydrophila</named-content> Responsible for Disease Epidemics in United States-Farmed Catfish

ABSTRACT Since 2009, catfish farming in the southeastern United States has been severely impacted by a highly virulent and clonal population of Aeromonas hydrophila causing motile Aeromonas septicemia (MAS) in catfish. The possible origin of this newly emerged highly virulent A. hydrophila strain is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mohammad J. Hossain, Dawei Sun, Donald J. McGarey, Shannon Wrenn, Laura M. Alexander, Maria Elena Martino, Ye Xing, Jeffery S. Terhune, Mark R. Liles
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/529f55ade61b4080a2ed8bcf850630f9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:529f55ade61b4080a2ed8bcf850630f9
record_format	dspace
spelling	oai:doaj.org-article:529f55ade61b4080a2ed8bcf850630f92021-11-15T15:47:39ZAn Asian Origin of Virulent <named-content content-type="genus-species">Aeromonas hydrophila</named-content> Responsible for Disease Epidemics in United States-Farmed Catfish10.1128/mBio.00848-142150-7511https://doaj.org/article/529f55ade61b4080a2ed8bcf850630f92014-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00848-14https://doaj.org/toc/2150-7511ABSTRACT Since 2009, catfish farming in the southeastern United States has been severely impacted by a highly virulent and clonal population of Aeromonas hydrophila causing motile Aeromonas septicemia (MAS) in catfish. The possible origin of this newly emerged highly virulent A. hydrophila strain is unknown. In this study, we show using whole-genome sequencing and comparative genomics that A. hydrophila isolates from diseased grass carp in China and catfish in the United States have highly similar genomes. Our phylogenomic analyses suggest that U.S. catfish isolates emerged from A. hydrophila populations of Asian origin. Furthermore, we identified an A. hydrophila strain isolated in 2004 from a diseased catfish in Mississippi, prior to the onset of the major epidemic outbreaks in Alabama starting in 2009, with genomic characteristics that are intermediate between those of the Asian and Alabama fish isolates. Investigation of A. hydrophila strain virulence demonstrated that the isolate from the U.S. catfish epidemic is significantly more virulent to both channel catfish and grass carp than is the Chinese carp isolate. This study implicates the importation of fish or fishery products into the United States as the source of highly virulent A. hydrophila that has caused severe epidemic outbreaks in United States-farmed catfish and further demonstrates the potential for invasive animal species to disseminate bacterial pathogens worldwide. IMPORTANCE Catfish aquaculture farming in the southeastern United States has been severely affected by the emergence of virulent Aeromonas hydrophila responsible for epidemic disease outbreaks, resulting in the death of over 10 million pounds of catfish. Because the origin of this newly emerged A. hydrophila strain is unknown, this study used a comparative genomics approach to conduct a phylogenomic analysis of A. hydrophila isolates obtained from the United States and Asia. Our results suggest that the virulent isolates from United States-farmed catfish have a recent common ancestor with A. hydrophila isolates from diseased Asian carp. We have also observed that an Asian carp isolate, like recent U.S. catfish isolates, is virulent in catfish. The results from this study suggest that the highly virulent U.S. epidemic isolates emerged from an Asian source and provide another example of the threat that invasive species pose in the dissemination of bacterial pathogens.Mohammad J. HossainDawei SunDonald J. McGareyShannon WrennLaura M. AlexanderMaria Elena MartinoYe XingJeffery S. TerhuneMark R. LilesAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 3 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Mohammad J. Hossain Dawei Sun Donald J. McGarey Shannon Wrenn Laura M. Alexander Maria Elena Martino Ye Xing Jeffery S. Terhune Mark R. Liles An Asian Origin of Virulent <named-content content-type="genus-species">Aeromonas hydrophila</named-content> Responsible for Disease Epidemics in United States-Farmed Catfish
description	ABSTRACT Since 2009, catfish farming in the southeastern United States has been severely impacted by a highly virulent and clonal population of Aeromonas hydrophila causing motile Aeromonas septicemia (MAS) in catfish. The possible origin of this newly emerged highly virulent A. hydrophila strain is unknown. In this study, we show using whole-genome sequencing and comparative genomics that A. hydrophila isolates from diseased grass carp in China and catfish in the United States have highly similar genomes. Our phylogenomic analyses suggest that U.S. catfish isolates emerged from A. hydrophila populations of Asian origin. Furthermore, we identified an A. hydrophila strain isolated in 2004 from a diseased catfish in Mississippi, prior to the onset of the major epidemic outbreaks in Alabama starting in 2009, with genomic characteristics that are intermediate between those of the Asian and Alabama fish isolates. Investigation of A. hydrophila strain virulence demonstrated that the isolate from the U.S. catfish epidemic is significantly more virulent to both channel catfish and grass carp than is the Chinese carp isolate. This study implicates the importation of fish or fishery products into the United States as the source of highly virulent A. hydrophila that has caused severe epidemic outbreaks in United States-farmed catfish and further demonstrates the potential for invasive animal species to disseminate bacterial pathogens worldwide. IMPORTANCE Catfish aquaculture farming in the southeastern United States has been severely affected by the emergence of virulent Aeromonas hydrophila responsible for epidemic disease outbreaks, resulting in the death of over 10 million pounds of catfish. Because the origin of this newly emerged A. hydrophila strain is unknown, this study used a comparative genomics approach to conduct a phylogenomic analysis of A. hydrophila isolates obtained from the United States and Asia. Our results suggest that the virulent isolates from United States-farmed catfish have a recent common ancestor with A. hydrophila isolates from diseased Asian carp. We have also observed that an Asian carp isolate, like recent U.S. catfish isolates, is virulent in catfish. The results from this study suggest that the highly virulent U.S. epidemic isolates emerged from an Asian source and provide another example of the threat that invasive species pose in the dissemination of bacterial pathogens.
format	article
author	Mohammad J. Hossain Dawei Sun Donald J. McGarey Shannon Wrenn Laura M. Alexander Maria Elena Martino Ye Xing Jeffery S. Terhune Mark R. Liles
author_facet	Mohammad J. Hossain Dawei Sun Donald J. McGarey Shannon Wrenn Laura M. Alexander Maria Elena Martino Ye Xing Jeffery S. Terhune Mark R. Liles
author_sort	Mohammad J. Hossain
title	An Asian Origin of Virulent <named-content content-type="genus-species">Aeromonas hydrophila</named-content> Responsible for Disease Epidemics in United States-Farmed Catfish
title_short	An Asian Origin of Virulent <named-content content-type="genus-species">Aeromonas hydrophila</named-content> Responsible for Disease Epidemics in United States-Farmed Catfish
title_full	An Asian Origin of Virulent <named-content content-type="genus-species">Aeromonas hydrophila</named-content> Responsible for Disease Epidemics in United States-Farmed Catfish
title_fullStr	An Asian Origin of Virulent <named-content content-type="genus-species">Aeromonas hydrophila</named-content> Responsible for Disease Epidemics in United States-Farmed Catfish
title_full_unstemmed	An Asian Origin of Virulent <named-content content-type="genus-species">Aeromonas hydrophila</named-content> Responsible for Disease Epidemics in United States-Farmed Catfish
title_sort	asian origin of virulent <named-content content-type="genus-species">aeromonas hydrophila</named-content> responsible for disease epidemics in united states-farmed catfish
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/529f55ade61b4080a2ed8bcf850630f9
work_keys_str_mv	AT mohammadjhossain anasianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT daweisun anasianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT donaldjmcgarey anasianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT shannonwrenn anasianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT lauramalexander anasianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT mariaelenamartino anasianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT yexing anasianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT jefferysterhune anasianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT markrliles anasianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT mohammadjhossain asianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT daweisun asianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT donaldjmcgarey asianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT shannonwrenn asianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT lauramalexander asianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT mariaelenamartino asianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT yexing asianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT jefferysterhune asianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish AT markrliles asianoriginofvirulentnamedcontentcontenttypegenusspeciesaeromonashydrophilanamedcontentresponsiblefordiseaseepidemicsinunitedstatesfarmedcatfish
_version_	1718427518811242496

An Asian Origin of Virulent <named-content content-type="genus-species">Aeromonas hydrophila</named-content> Responsible for Disease Epidemics in United States-Farmed Catfish

Ejemplares similares