Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase

Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein’s surface...

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Autores principales:	Marija D. Stanišić, Nikolina Popović Kokar, Predrag Ristić, Ana Marija Balaž, Milan Senćanski, Miloš Ognjanović, Veljko R. Đokić, Radivoje Prodanović, Tamara R. Todorović
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	metal–organic frameworks ZIF-8 biomimetic mineralization biocomposites biocatalysts Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/52a1d6f1776d453aa8f977b0b9dae5b0
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spelling	oai:doaj.org-article:52a1d6f1776d453aa8f977b0b9dae5b02021-11-25T18:47:50ZChemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase10.3390/polym132238752073-4360https://doaj.org/article/52a1d6f1776d453aa8f977b0b9dae5b02021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/3875https://doaj.org/toc/2073-4360Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein’s surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite crystal formation. However, a chemical modification of carbohydrate components by periodate oxidation for glycoproteins can serve as an alternative strategy. In the present study, an industrially important enzyme glucose oxidase (GOx) was selected as a model system. Periodate oxidation of GOx by 2.5 mM sodium periodate increased negative charge on the enzyme molecule, from −10.2 to −36.9 mV, as shown by zeta potential measurements and native PAGE electrophoresis. Biomineralization experiments with oxidized GOx resulted in higher specific activity, effectiveness factor, and higher thermostability of the ZIF-8 biocomposites. Periodate oxidation of carbohydrate components for glycoproteins can serve as a facile and general method for facilitating the biomimetic mineralization of other industrially relevant glycoproteins.Marija D. StanišićNikolina Popović KokarPredrag RistićAna Marija BalažMilan SenćanskiMiloš OgnjanovićVeljko R. ĐokićRadivoje ProdanovićTamara R. TodorovićMDPI AGarticlemetal–organic frameworksZIF-8biomimetic mineralizationbiocompositesbiocatalystsOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3875, p 3875 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	metal–organic frameworks ZIF-8 biomimetic mineralization biocomposites biocatalysts Organic chemistry QD241-441
spellingShingle	metal–organic frameworks ZIF-8 biomimetic mineralization biocomposites biocatalysts Organic chemistry QD241-441 Marija D. Stanišić Nikolina Popović Kokar Predrag Ristić Ana Marija Balaž Milan Senćanski Miloš Ognjanović Veljko R. Đokić Radivoje Prodanović Tamara R. Todorović Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase
description	Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein’s surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite crystal formation. However, a chemical modification of carbohydrate components by periodate oxidation for glycoproteins can serve as an alternative strategy. In the present study, an industrially important enzyme glucose oxidase (GOx) was selected as a model system. Periodate oxidation of GOx by 2.5 mM sodium periodate increased negative charge on the enzyme molecule, from −10.2 to −36.9 mV, as shown by zeta potential measurements and native PAGE electrophoresis. Biomineralization experiments with oxidized GOx resulted in higher specific activity, effectiveness factor, and higher thermostability of the ZIF-8 biocomposites. Periodate oxidation of carbohydrate components for glycoproteins can serve as a facile and general method for facilitating the biomimetic mineralization of other industrially relevant glycoproteins.
format	article
author	Marija D. Stanišić Nikolina Popović Kokar Predrag Ristić Ana Marija Balaž Milan Senćanski Miloš Ognjanović Veljko R. Đokić Radivoje Prodanović Tamara R. Todorović
author_facet	Marija D. Stanišić Nikolina Popović Kokar Predrag Ristić Ana Marija Balaž Milan Senćanski Miloš Ognjanović Veljko R. Đokić Radivoje Prodanović Tamara R. Todorović
author_sort	Marija D. Stanišić
title	Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase
title_short	Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase
title_full	Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase
title_fullStr	Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase
title_full_unstemmed	Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase
title_sort	chemical modification of glycoproteins’ carbohydrate moiety as a general strategy for the synthesis of efficient biocatalysts by biomimetic mineralization: the case of glucose oxidase
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/52a1d6f1776d453aa8f977b0b9dae5b0
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Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase

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