Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes

Abstract The identification of brown adipose deposits in adults has led to significant interest in targeting this metabolically active tissue for treatment of obesity and diabetes. Improved methods for the direct measurement of heat production as the signature function of brown adipocytes (BAs), par...

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Autores principales:	Rókus Kriszt, Satoshi Arai, Hideki Itoh, Michelle H. Lee, Anna G. Goralczyk, Xiu Min Ang, Aaron M. Cypess, Andrew P. White, Farnaz Shamsi, Ruidan Xue, Jung Yeol Lee, Sung-Chan Lee, Yanyan Hou, Tetsuya Kitaguchi, Thankiah Sudhaharan, Shin’ichi Ishiwata, E. Birgitte Lane, Young-Tae Chang, Yu-Hua Tseng, Madoka Suzuki, Michael Raghunath
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/52a314579272494397c0203168538870
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spelling	oai:doaj.org-article:52a314579272494397c02031685388702021-12-02T12:32:53ZOptical visualisation of thermogenesis in stimulated single-cell brown adipocytes10.1038/s41598-017-00291-92045-2322https://doaj.org/article/52a314579272494397c02031685388702017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00291-9https://doaj.org/toc/2045-2322Abstract The identification of brown adipose deposits in adults has led to significant interest in targeting this metabolically active tissue for treatment of obesity and diabetes. Improved methods for the direct measurement of heat production as the signature function of brown adipocytes (BAs), particularly at the single cell level, would be of substantial benefit to these ongoing efforts. Here, we report the first application of a small molecule-type thermosensitive fluorescent dye, ERthermAC, to monitor thermogenesis in BAs derived from murine brown fat precursors and in human brown fat cells differentiated from human neck brown preadipocytes. ERthermAC accumulated in the endoplasmic reticulum of BAs and displayed a marked change in fluorescence intensity in response to adrenergic stimulation of cells, which corresponded to temperature change. ERthermAC fluorescence intensity profiles were congruent with mitochondrial depolarisation events visualised by the JC-1 probe. Moreover, the averaged fluorescence intensity changes across a population of cells correlated well with dynamic changes such as thermal power, oxygen consumption, and extracellular acidification rates. These findings suggest ERthermAC as a promising new tool for studying thermogenic function in brown adipocytes of both murine and human origins.Rókus KrisztSatoshi AraiHideki ItohMichelle H. LeeAnna G. GoralczykXiu Min AngAaron M. CypessAndrew P. WhiteFarnaz ShamsiRuidan XueJung Yeol LeeSung-Chan LeeYanyan HouTetsuya KitaguchiThankiah SudhaharanShin’ichi IshiwataE. Birgitte LaneYoung-Tae ChangYu-Hua TsengMadoka SuzukiMichael RaghunathNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rókus Kriszt Satoshi Arai Hideki Itoh Michelle H. Lee Anna G. Goralczyk Xiu Min Ang Aaron M. Cypess Andrew P. White Farnaz Shamsi Ruidan Xue Jung Yeol Lee Sung-Chan Lee Yanyan Hou Tetsuya Kitaguchi Thankiah Sudhaharan Shin’ichi Ishiwata E. Birgitte Lane Young-Tae Chang Yu-Hua Tseng Madoka Suzuki Michael Raghunath Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes
description	Abstract The identification of brown adipose deposits in adults has led to significant interest in targeting this metabolically active tissue for treatment of obesity and diabetes. Improved methods for the direct measurement of heat production as the signature function of brown adipocytes (BAs), particularly at the single cell level, would be of substantial benefit to these ongoing efforts. Here, we report the first application of a small molecule-type thermosensitive fluorescent dye, ERthermAC, to monitor thermogenesis in BAs derived from murine brown fat precursors and in human brown fat cells differentiated from human neck brown preadipocytes. ERthermAC accumulated in the endoplasmic reticulum of BAs and displayed a marked change in fluorescence intensity in response to adrenergic stimulation of cells, which corresponded to temperature change. ERthermAC fluorescence intensity profiles were congruent with mitochondrial depolarisation events visualised by the JC-1 probe. Moreover, the averaged fluorescence intensity changes across a population of cells correlated well with dynamic changes such as thermal power, oxygen consumption, and extracellular acidification rates. These findings suggest ERthermAC as a promising new tool for studying thermogenic function in brown adipocytes of both murine and human origins.
format	article
author	Rókus Kriszt Satoshi Arai Hideki Itoh Michelle H. Lee Anna G. Goralczyk Xiu Min Ang Aaron M. Cypess Andrew P. White Farnaz Shamsi Ruidan Xue Jung Yeol Lee Sung-Chan Lee Yanyan Hou Tetsuya Kitaguchi Thankiah Sudhaharan Shin’ichi Ishiwata E. Birgitte Lane Young-Tae Chang Yu-Hua Tseng Madoka Suzuki Michael Raghunath
author_facet	Rókus Kriszt Satoshi Arai Hideki Itoh Michelle H. Lee Anna G. Goralczyk Xiu Min Ang Aaron M. Cypess Andrew P. White Farnaz Shamsi Ruidan Xue Jung Yeol Lee Sung-Chan Lee Yanyan Hou Tetsuya Kitaguchi Thankiah Sudhaharan Shin’ichi Ishiwata E. Birgitte Lane Young-Tae Chang Yu-Hua Tseng Madoka Suzuki Michael Raghunath
author_sort	Rókus Kriszt
title	Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes
title_short	Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes
title_full	Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes
title_fullStr	Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes
title_full_unstemmed	Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes
title_sort	optical visualisation of thermogenesis in stimulated single-cell brown adipocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/52a314579272494397c0203168538870
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Optical visualisation of thermogenesis in stimulated single-cell brown adipocytes

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