Near-infrared quantum dots for HER2 localization and imaging of cancer cells

Sarwat B Rizvi,1 Sepideh Rouhi,1 Shohei Taniguchi,2 Shi Yu Yang,1 Mark Green,2 Mo Keshtgar,1,3 Alexander M Seifalian1,3 1UCL Centre for Nanotechnology and Regenerative Medicine, University College London, 2Department of Physics, King's College London, 3Royal Free London NHS Foundation Trust...

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Autores principales:	Rizvi SB, Rouhi S, Taniguchi S, Yang SY, Green M, Keshtgar M, Seifalian AM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/52a48702e73f4219a09520aaf48a1b49
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spelling	oai:doaj.org-article:52a48702e73f4219a09520aaf48a1b492021-12-02T02:14:38ZNear-infrared quantum dots for HER2 localization and imaging of cancer cells1178-2013https://doaj.org/article/52a48702e73f4219a09520aaf48a1b492014-03-01T00:00:00Zhttp://www.dovepress.com/near-infrared-quantum-dots-for-her2-localization-and-imaging-of-cancer-a16078https://doaj.org/toc/1178-2013 Sarwat B Rizvi,1 Sepideh Rouhi,1 Shohei Taniguchi,2 Shi Yu Yang,1 Mark Green,2 Mo Keshtgar,1,3 Alexander M Seifalian1,3 1UCL Centre for Nanotechnology and Regenerative Medicine, University College London, 2Department of Physics, King's College London, 3Royal Free London NHS Foundation Trust Hospital, London, UK Background: Quantum dots are fluorescent nanoparticles with unique photophysical properties that allow them to be used as diagnostic, therapeutic, and theranostic agents, particularly in medical and surgical oncology. Near-infrared-emitting quantum dots can be visualized in deep tissues because the biological window is transparent to these wavelengths. Their small sizes and free surface reactive groups that can be conjugated to biomolecules make them ideal probes for in vivo cancer localization, targeted chemotherapy, and image-guided cancer surgery. The human epidermal growth factor receptor 2 gene (HER2/neu) is overexpressed in 25%–30% of breast cancers. The current methods of detection for HER2 status, including immunohistochemistry and fluorescence in situ hybridization, are used ex vivo and cannot be used in vivo. In this paper, we demonstrate the application of near-infrared-emitting quantum dots for HER2 localization in fixed and live cancer cells as a first step prior to their in vivo application. Methods: Near-infrared-emitting quantum dots were characterized and their in vitro toxicity was established using three cancer cell lines, ie, HepG2, SK-BR-3 (HER2-overexpressing), and MCF7 (HER2-underexpressing). Mouse antihuman anti-HER2 monoclonal antibody was conjugated to the near-infrared-emitting quantum dots. Results: In vitro toxicity studies showed biocompatibility of SK-BR-3 and MCF7 cell lines with near-infrared-emitting quantum dots at a concentration of 60 µg/mL after one hour and 24 hours of exposure. Near-infrared-emitting quantum dot antiHER2-antibody bioconjugates successfully localized HER2 receptors on SK-BR-3 cells. Conclusion: Near-infrared-emitting quantum dot bioconjugates can be used for rapid localization of HER2 receptors and can potentially be used for targeted therapy as well as image-guided surgery. Keywords: anti-HER2 antibody, HER2 localization, quantum dots, in vitro imaging, nanotechnology, cancerRizvi SBRouhi STaniguchi SYang SYGreen MKeshtgar MSeifalian AMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1323-1337 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Rizvi SB Rouhi S Taniguchi S Yang SY Green M Keshtgar M Seifalian AM Near-infrared quantum dots for HER2 localization and imaging of cancer cells
description	Sarwat B Rizvi,1 Sepideh Rouhi,1 Shohei Taniguchi,2 Shi Yu Yang,1 Mark Green,2 Mo Keshtgar,1,3 Alexander M Seifalian1,3 1UCL Centre for Nanotechnology and Regenerative Medicine, University College London, 2Department of Physics, King's College London, 3Royal Free London NHS Foundation Trust Hospital, London, UK Background: Quantum dots are fluorescent nanoparticles with unique photophysical properties that allow them to be used as diagnostic, therapeutic, and theranostic agents, particularly in medical and surgical oncology. Near-infrared-emitting quantum dots can be visualized in deep tissues because the biological window is transparent to these wavelengths. Their small sizes and free surface reactive groups that can be conjugated to biomolecules make them ideal probes for in vivo cancer localization, targeted chemotherapy, and image-guided cancer surgery. The human epidermal growth factor receptor 2 gene (HER2/neu) is overexpressed in 25%–30% of breast cancers. The current methods of detection for HER2 status, including immunohistochemistry and fluorescence in situ hybridization, are used ex vivo and cannot be used in vivo. In this paper, we demonstrate the application of near-infrared-emitting quantum dots for HER2 localization in fixed and live cancer cells as a first step prior to their in vivo application. Methods: Near-infrared-emitting quantum dots were characterized and their in vitro toxicity was established using three cancer cell lines, ie, HepG2, SK-BR-3 (HER2-overexpressing), and MCF7 (HER2-underexpressing). Mouse antihuman anti-HER2 monoclonal antibody was conjugated to the near-infrared-emitting quantum dots. Results: In vitro toxicity studies showed biocompatibility of SK-BR-3 and MCF7 cell lines with near-infrared-emitting quantum dots at a concentration of 60 µg/mL after one hour and 24 hours of exposure. Near-infrared-emitting quantum dot antiHER2-antibody bioconjugates successfully localized HER2 receptors on SK-BR-3 cells. Conclusion: Near-infrared-emitting quantum dot bioconjugates can be used for rapid localization of HER2 receptors and can potentially be used for targeted therapy as well as image-guided surgery. Keywords: anti-HER2 antibody, HER2 localization, quantum dots, in vitro imaging, nanotechnology, cancer
format	article
author	Rizvi SB Rouhi S Taniguchi S Yang SY Green M Keshtgar M Seifalian AM
author_facet	Rizvi SB Rouhi S Taniguchi S Yang SY Green M Keshtgar M Seifalian AM
author_sort	Rizvi SB
title	Near-infrared quantum dots for HER2 localization and imaging of cancer cells
title_short	Near-infrared quantum dots for HER2 localization and imaging of cancer cells
title_full	Near-infrared quantum dots for HER2 localization and imaging of cancer cells
title_fullStr	Near-infrared quantum dots for HER2 localization and imaging of cancer cells
title_full_unstemmed	Near-infrared quantum dots for HER2 localization and imaging of cancer cells
title_sort	near-infrared quantum dots for her2 localization and imaging of cancer cells
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/52a48702e73f4219a09520aaf48a1b49
work_keys_str_mv	AT rizvisb nearinfraredquantumdotsforher2localizationandimagingofcancercells AT rouhis nearinfraredquantumdotsforher2localizationandimagingofcancercells AT taniguchis nearinfraredquantumdotsforher2localizationandimagingofcancercells AT yangsy nearinfraredquantumdotsforher2localizationandimagingofcancercells AT greenm nearinfraredquantumdotsforher2localizationandimagingofcancercells AT keshtgarm nearinfraredquantumdotsforher2localizationandimagingofcancercells AT seifalianam nearinfraredquantumdotsforher2localizationandimagingofcancercells
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Near-infrared quantum dots for HER2 localization and imaging of cancer cells

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