Biodiversity of the intestinal microbiota of black tiger prawn, Penaeus monodon, increases with age and is only transiently impacted by major ingredient replacement in the diet

Substitution of aquaculture feed ingredients to reduce reliance on capture fisheries may affect gut microbiome and resilience to environmental stress. To investigate replacement protein sources in feeds for black tiger prawn, Penaeus monodon, three isonitrogenous, isoenergetic diets were tested in a...

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Autores principales:	Minami Kawasaki, Ting Him Wallace Lo, Viviana Lazzarotto, Matthew Briggs, Richard P. Smullen, Andrew C. Barnes
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Penaeus monodon Fishmeal Microbiome Compositional analysis Diet Aquaculture. Fisheries. Angling SH1-691
Acceso en línea:	https://doaj.org/article/52a4b57cba1b48679d85e78d0ec91061
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spelling	oai:doaj.org-article:52a4b57cba1b48679d85e78d0ec910612021-11-20T05:07:22ZBiodiversity of the intestinal microbiota of black tiger prawn, Penaeus monodon, increases with age and is only transiently impacted by major ingredient replacement in the diet2352-513410.1016/j.aqrep.2021.100948https://doaj.org/article/52a4b57cba1b48679d85e78d0ec910612022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352513421003653https://doaj.org/toc/2352-5134Substitution of aquaculture feed ingredients to reduce reliance on capture fisheries may affect gut microbiome and resilience to environmental stress. To investigate replacement protein sources in feeds for black tiger prawn, Penaeus monodon, three isonitrogenous, isoenergetic diets were tested in a randomised blind trial using fisheries by-product meal, cattle and poultry by-product meal and soybean meal as the major protein. Juvenile P. monodon (1.46 ± 0.28 g, 24 per tank, 4 tanks per treatment) were acclimatised on reference diet for 2 weeks, weighed, then fed experimental diets for two weeks. Elevated temperature, pH and salinity was applied for a further two weeks. Feed conversion ratio was significantly higher in animals fed soybean meal-based diet (F (2,9) = 21.7, p < 0.001). To determine any effect of diet on intestinal microbiome the V6-V8 region of the 16s rRNA gene was sequenced on arrival from the farm, at the end of acclimatisation, 24 h after starting test feeds, after 2 weeks on test feeds and after stress period. We found no significant effect of diet on alpha diversity of the midgut microbiota, and no correlation between stress resilience, diet and gut microbiome diversity. However, time had a significant impact on beta diversity throughout the experiment across all three diets (p < 0.001), with the most abundant genera on arrival from the farm being from the genera Photobacterium and Vibrio while the relative abundance of members of the Rhodobacteraceae, Pseudoalteromonadaceae, Mycoplasmataceae and Flavobacteriaceae increased throughout the trial and were also found in the seawater supply. Changing only the protein in the diet is not sufficient to impact gut microbiome, even when animal growth and feed conversion is severely compromised by the diet. We found that time/age is the major driver of intestinal microbiome changes in P. monodon, with diversity increasing as the animal ages.Minami KawasakiTing Him Wallace LoViviana LazzarottoMatthew BriggsRichard P. SmullenAndrew C. BarnesElsevierarticlePenaeus monodonFishmealMicrobiomeCompositional analysisDietAquaculture. Fisheries. AnglingSH1-691ENAquaculture Reports, Vol 22, Iss , Pp 100948- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Penaeus monodon Fishmeal Microbiome Compositional analysis Diet Aquaculture. Fisheries. Angling SH1-691
spellingShingle	Penaeus monodon Fishmeal Microbiome Compositional analysis Diet Aquaculture. Fisheries. Angling SH1-691 Minami Kawasaki Ting Him Wallace Lo Viviana Lazzarotto Matthew Briggs Richard P. Smullen Andrew C. Barnes Biodiversity of the intestinal microbiota of black tiger prawn, Penaeus monodon, increases with age and is only transiently impacted by major ingredient replacement in the diet
description	Substitution of aquaculture feed ingredients to reduce reliance on capture fisheries may affect gut microbiome and resilience to environmental stress. To investigate replacement protein sources in feeds for black tiger prawn, Penaeus monodon, three isonitrogenous, isoenergetic diets were tested in a randomised blind trial using fisheries by-product meal, cattle and poultry by-product meal and soybean meal as the major protein. Juvenile P. monodon (1.46 ± 0.28 g, 24 per tank, 4 tanks per treatment) were acclimatised on reference diet for 2 weeks, weighed, then fed experimental diets for two weeks. Elevated temperature, pH and salinity was applied for a further two weeks. Feed conversion ratio was significantly higher in animals fed soybean meal-based diet (F (2,9) = 21.7, p < 0.001). To determine any effect of diet on intestinal microbiome the V6-V8 region of the 16s rRNA gene was sequenced on arrival from the farm, at the end of acclimatisation, 24 h after starting test feeds, after 2 weeks on test feeds and after stress period. We found no significant effect of diet on alpha diversity of the midgut microbiota, and no correlation between stress resilience, diet and gut microbiome diversity. However, time had a significant impact on beta diversity throughout the experiment across all three diets (p < 0.001), with the most abundant genera on arrival from the farm being from the genera Photobacterium and Vibrio while the relative abundance of members of the Rhodobacteraceae, Pseudoalteromonadaceae, Mycoplasmataceae and Flavobacteriaceae increased throughout the trial and were also found in the seawater supply. Changing only the protein in the diet is not sufficient to impact gut microbiome, even when animal growth and feed conversion is severely compromised by the diet. We found that time/age is the major driver of intestinal microbiome changes in P. monodon, with diversity increasing as the animal ages.
format	article
author	Minami Kawasaki Ting Him Wallace Lo Viviana Lazzarotto Matthew Briggs Richard P. Smullen Andrew C. Barnes
author_facet	Minami Kawasaki Ting Him Wallace Lo Viviana Lazzarotto Matthew Briggs Richard P. Smullen Andrew C. Barnes
author_sort	Minami Kawasaki
title	Biodiversity of the intestinal microbiota of black tiger prawn, Penaeus monodon, increases with age and is only transiently impacted by major ingredient replacement in the diet
title_short	Biodiversity of the intestinal microbiota of black tiger prawn, Penaeus monodon, increases with age and is only transiently impacted by major ingredient replacement in the diet
title_full	Biodiversity of the intestinal microbiota of black tiger prawn, Penaeus monodon, increases with age and is only transiently impacted by major ingredient replacement in the diet
title_fullStr	Biodiversity of the intestinal microbiota of black tiger prawn, Penaeus monodon, increases with age and is only transiently impacted by major ingredient replacement in the diet
title_full_unstemmed	Biodiversity of the intestinal microbiota of black tiger prawn, Penaeus monodon, increases with age and is only transiently impacted by major ingredient replacement in the diet
title_sort	biodiversity of the intestinal microbiota of black tiger prawn, penaeus monodon, increases with age and is only transiently impacted by major ingredient replacement in the diet
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/52a4b57cba1b48679d85e78d0ec91061
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Biodiversity of the intestinal microbiota of black tiger prawn, Penaeus monodon, increases with age and is only transiently impacted by major ingredient replacement in the diet

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