Proteomics study on the effect of silybin on cardiomyopathy in obese mice

Abstract Due to the increase in the number of obese individuals, the incidence of obesity-related complications such as cardiovascular disease and type 2 diabetes is higher. The aim of the present study was to explore the effects of silybin on protein expression in obese mice. Firstly, serum was col...

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Autores principales:	Fei Wang, Zelin Li, Tiantian Song, Yujiao Jia, Licui Qi, Luping Ren, Shuchun Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/52a4bfd7d3b4470fae0fe7bd1ff52434
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spelling	oai:doaj.org-article:52a4bfd7d3b4470fae0fe7bd1ff524342021-12-02T14:25:32ZProteomics study on the effect of silybin on cardiomyopathy in obese mice10.1038/s41598-021-86717-x2045-2322https://doaj.org/article/52a4bfd7d3b4470fae0fe7bd1ff524342021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86717-xhttps://doaj.org/toc/2045-2322Abstract Due to the increase in the number of obese individuals, the incidence of obesity-related complications such as cardiovascular disease and type 2 diabetes is higher. The aim of the present study was to explore the effects of silybin on protein expression in obese mice. Firstly, serum was collected, and it was used to detect serum lipids and other serological indicators. Secondly, total protein from epididymal adipose tissue was extracted for differential expression analysis by quantitative tandem mass tag (TMT) combined with liquid chromatography-tandem mass spectrometry (LC–MS/MS), followed by bioinformatics and protein–protein interaction (PPI) network analyses of these proteins. Lastly, real-time polymerase chain reaction (RT-PCR) and parallel reaction monitoring (PRM) were used to further validate the expression of identified differentially expressed proteins (DEPs) at the mRNA and protein level, respectively. The results revealed that silybin could improve abnormal lipid metabolism caused by the high fat diet in obese mice. A total of 341, 538 and 243 DEPs were found in the high fat/control (WF/WC), silybin/high fat (WS/WF) and WS/WC groups, respectively. These DEPs mainly participated in lipid metabolism and energy metabolism. Notably, tropomyosin 1 (TPM1), myosin light chain 2 (MYL2), myosin heavy chain 11 (MYH11) and other DEPs were involved in hypertrophic cardiomyopathy, dilated cardiomyopathy and other pathways. Silybin could protect cardiac function by inducing the protein expression of TPM1, MYL2 and MYH11 in the adipose tissue of obese mice.Fei WangZelin LiTiantian SongYujiao JiaLicui QiLuping RenShuchun ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fei Wang Zelin Li Tiantian Song Yujiao Jia Licui Qi Luping Ren Shuchun Chen Proteomics study on the effect of silybin on cardiomyopathy in obese mice
description	Abstract Due to the increase in the number of obese individuals, the incidence of obesity-related complications such as cardiovascular disease and type 2 diabetes is higher. The aim of the present study was to explore the effects of silybin on protein expression in obese mice. Firstly, serum was collected, and it was used to detect serum lipids and other serological indicators. Secondly, total protein from epididymal adipose tissue was extracted for differential expression analysis by quantitative tandem mass tag (TMT) combined with liquid chromatography-tandem mass spectrometry (LC–MS/MS), followed by bioinformatics and protein–protein interaction (PPI) network analyses of these proteins. Lastly, real-time polymerase chain reaction (RT-PCR) and parallel reaction monitoring (PRM) were used to further validate the expression of identified differentially expressed proteins (DEPs) at the mRNA and protein level, respectively. The results revealed that silybin could improve abnormal lipid metabolism caused by the high fat diet in obese mice. A total of 341, 538 and 243 DEPs were found in the high fat/control (WF/WC), silybin/high fat (WS/WF) and WS/WC groups, respectively. These DEPs mainly participated in lipid metabolism and energy metabolism. Notably, tropomyosin 1 (TPM1), myosin light chain 2 (MYL2), myosin heavy chain 11 (MYH11) and other DEPs were involved in hypertrophic cardiomyopathy, dilated cardiomyopathy and other pathways. Silybin could protect cardiac function by inducing the protein expression of TPM1, MYL2 and MYH11 in the adipose tissue of obese mice.
format	article
author	Fei Wang Zelin Li Tiantian Song Yujiao Jia Licui Qi Luping Ren Shuchun Chen
author_facet	Fei Wang Zelin Li Tiantian Song Yujiao Jia Licui Qi Luping Ren Shuchun Chen
author_sort	Fei Wang
title	Proteomics study on the effect of silybin on cardiomyopathy in obese mice
title_short	Proteomics study on the effect of silybin on cardiomyopathy in obese mice
title_full	Proteomics study on the effect of silybin on cardiomyopathy in obese mice
title_fullStr	Proteomics study on the effect of silybin on cardiomyopathy in obese mice
title_full_unstemmed	Proteomics study on the effect of silybin on cardiomyopathy in obese mice
title_sort	proteomics study on the effect of silybin on cardiomyopathy in obese mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/52a4bfd7d3b4470fae0fe7bd1ff52434
work_keys_str_mv	AT feiwang proteomicsstudyontheeffectofsilybinoncardiomyopathyinobesemice AT zelinli proteomicsstudyontheeffectofsilybinoncardiomyopathyinobesemice AT tiantiansong proteomicsstudyontheeffectofsilybinoncardiomyopathyinobesemice AT yujiaojia proteomicsstudyontheeffectofsilybinoncardiomyopathyinobesemice AT licuiqi proteomicsstudyontheeffectofsilybinoncardiomyopathyinobesemice AT lupingren proteomicsstudyontheeffectofsilybinoncardiomyopathyinobesemice AT shuchunchen proteomicsstudyontheeffectofsilybinoncardiomyopathyinobesemice
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Proteomics study on the effect of silybin on cardiomyopathy in obese mice

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