Distribution and functions of TonB-dependent transporters in marine bacteria and environments: implications for dissolved organic matter utilization.

<h4>Background</h4>Bacteria play critical roles in marine nutrient cycles by incorporating and redistributing dissolved organic matter (DOM) and inorganic nutrients in the ocean. TonB-dependent transporter (TBDT) proteins allow Gram-negative bacteria to take up scarce resources from nutr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kai Tang, Nianzhi Jiao, Keshao Liu, Yao Zhang, Shuhui Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/52b37f04296e4148a875f32027b00453
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:52b37f04296e4148a875f32027b00453
record_format	dspace
spelling	oai:doaj.org-article:52b37f04296e4148a875f32027b004532021-11-18T07:11:49ZDistribution and functions of TonB-dependent transporters in marine bacteria and environments: implications for dissolved organic matter utilization.1932-620310.1371/journal.pone.0041204https://doaj.org/article/52b37f04296e4148a875f32027b004532012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22829928/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Bacteria play critical roles in marine nutrient cycles by incorporating and redistributing dissolved organic matter (DOM) and inorganic nutrients in the ocean. TonB-dependent transporter (TBDT) proteins allow Gram-negative bacteria to take up scarce resources from nutrient-limiting environments as well as siderophores, heme, vitamin B12, and recently identified carbohydrates. Thus, the characterization of TBDT distribution and functions is essential to better understand the contribution TBDT to DOM assimilation and its consequences on nutrient cycling in the environment.<h4>Methodology/principal findings</h4>This study presents the distribution of encoded known and putative TBDT proteins in the genomes of microorganisms and from the Global Ocean Survey data. Using a Lek clustering algorithm and substrate specificities, the TBDT sequences were mainly classified into the following three groups: (1) DOM transporters; (2) Siderophores/Vitamins transporters; and (3) Heme/Hemophores/Iron(heme)-binding protein transporters. Diverse TBDTs were found in the genomes of oligotroph Citromicrobium bathyomarinum JL354 and Citromicrobium sp JLT1363 and were highly expressed in the stationary phase of bacterial growth. The results show that the Gammaproteobacteria and the Cytophaga-Flavobacterium-Bacteroides (CFB) group bacteria accounted for the majority of the TBDT gene pool in marine surface waters.<h4>Conclusions/significance</h4>The results of this study confirm the ecological importance of TBDTs in DOM assimilation for bacteria in marine environments owing to a wide range of substrate utilization potential in the ubiquitous Gammaproteobacteria and CFB group bacteria.Kai TangNianzhi JiaoKeshao LiuYao ZhangShuhui LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41204 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai Tang Nianzhi Jiao Keshao Liu Yao Zhang Shuhui Li Distribution and functions of TonB-dependent transporters in marine bacteria and environments: implications for dissolved organic matter utilization.
description	<h4>Background</h4>Bacteria play critical roles in marine nutrient cycles by incorporating and redistributing dissolved organic matter (DOM) and inorganic nutrients in the ocean. TonB-dependent transporter (TBDT) proteins allow Gram-negative bacteria to take up scarce resources from nutrient-limiting environments as well as siderophores, heme, vitamin B12, and recently identified carbohydrates. Thus, the characterization of TBDT distribution and functions is essential to better understand the contribution TBDT to DOM assimilation and its consequences on nutrient cycling in the environment.<h4>Methodology/principal findings</h4>This study presents the distribution of encoded known and putative TBDT proteins in the genomes of microorganisms and from the Global Ocean Survey data. Using a Lek clustering algorithm and substrate specificities, the TBDT sequences were mainly classified into the following three groups: (1) DOM transporters; (2) Siderophores/Vitamins transporters; and (3) Heme/Hemophores/Iron(heme)-binding protein transporters. Diverse TBDTs were found in the genomes of oligotroph Citromicrobium bathyomarinum JL354 and Citromicrobium sp JLT1363 and were highly expressed in the stationary phase of bacterial growth. The results show that the Gammaproteobacteria and the Cytophaga-Flavobacterium-Bacteroides (CFB) group bacteria accounted for the majority of the TBDT gene pool in marine surface waters.<h4>Conclusions/significance</h4>The results of this study confirm the ecological importance of TBDTs in DOM assimilation for bacteria in marine environments owing to a wide range of substrate utilization potential in the ubiquitous Gammaproteobacteria and CFB group bacteria.
format	article
author	Kai Tang Nianzhi Jiao Keshao Liu Yao Zhang Shuhui Li
author_facet	Kai Tang Nianzhi Jiao Keshao Liu Yao Zhang Shuhui Li
author_sort	Kai Tang
title	Distribution and functions of TonB-dependent transporters in marine bacteria and environments: implications for dissolved organic matter utilization.
title_short	Distribution and functions of TonB-dependent transporters in marine bacteria and environments: implications for dissolved organic matter utilization.
title_full	Distribution and functions of TonB-dependent transporters in marine bacteria and environments: implications for dissolved organic matter utilization.
title_fullStr	Distribution and functions of TonB-dependent transporters in marine bacteria and environments: implications for dissolved organic matter utilization.
title_full_unstemmed	Distribution and functions of TonB-dependent transporters in marine bacteria and environments: implications for dissolved organic matter utilization.
title_sort	distribution and functions of tonb-dependent transporters in marine bacteria and environments: implications for dissolved organic matter utilization.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/52b37f04296e4148a875f32027b00453
work_keys_str_mv	AT kaitang distributionandfunctionsoftonbdependenttransportersinmarinebacteriaandenvironmentsimplicationsfordissolvedorganicmatterutilization AT nianzhijiao distributionandfunctionsoftonbdependenttransportersinmarinebacteriaandenvironmentsimplicationsfordissolvedorganicmatterutilization AT keshaoliu distributionandfunctionsoftonbdependenttransportersinmarinebacteriaandenvironmentsimplicationsfordissolvedorganicmatterutilization AT yaozhang distributionandfunctionsoftonbdependenttransportersinmarinebacteriaandenvironmentsimplicationsfordissolvedorganicmatterutilization AT shuhuili distributionandfunctionsoftonbdependenttransportersinmarinebacteriaandenvironmentsimplicationsfordissolvedorganicmatterutilization
_version_	1718423785582886912

Distribution and functions of TonB-dependent transporters in marine bacteria and environments: implications for dissolved organic matter utilization.

Ejemplares similares