Visualization of a hyaluronan network on the surface of silicone-hydrogel materials

Katarzyna A Wygladacz, Daniel J Hook Vision Care, Bausch & Lomb Incorporated, Rochester, NY, USA Abstract: Biotrue multipurpose solution (MPS) is a bioinspired disinfecting and conditioning solution that includes hyaluronic acid (HA) as a natural wetting agent. Previous studies demonstrate...

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Autores principales:	Wygladacz KA, Hook DJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	contact lens hyaluronan MPS AFM CSLM DIC microscopy Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/52b6af1f879045f48e25a30a0edbfa9e
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spelling	oai:doaj.org-article:52b6af1f879045f48e25a30a0edbfa9e2021-12-02T06:17:14ZVisualization of a hyaluronan network on the surface of silicone-hydrogel materials1177-5483https://doaj.org/article/52b6af1f879045f48e25a30a0edbfa9e2016-07-01T00:00:00Zhttps://www.dovepress.com/visualization-of-a-hyaluronan-network-on-the-surface-of-silicone-hydro-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Katarzyna A Wygladacz, Daniel J Hook Vision Care, Bausch & Lomb Incorporated, Rochester, NY, USA Abstract: Biotrue multipurpose solution (MPS) is a bioinspired disinfecting and conditioning solution that includes hyaluronic acid (HA) as a natural wetting agent. Previous studies demonstrated that HA sorbed from Biotrue MPS on both conventional and silicone hydrogel (SiHy) contact lens materials; an in vitro simulated-wear test validated the presence of HA on the lens surfaces for as long as 20 hours. In this study, the morphology and distribution of HA sorbed from both Biotrue and pure HA solution on SiHy contact lens surfaces was examined. Atomic force microscopy imaging was used to illustrate the topography of fresh SiHy contact lens materials before and after incubation with 0.1% (w/v) HA solution. The distribution, as well as fine details of the HA network, were resolved by first staining HA with Gram’s safranin, then imaging with confocal laser-scanning microscopy and differential interference-contrast microscopy. In this approach, SiHy materials take up the dye (safranin) nonspecifically, such that the resultant safranin–HA complex appears dim against the fluorescent lens background. Balafilcon A was chosen as the representative of glassy SiHy lenses that require postpolymerization plasma treatment to increase wettability. Senofilcon A and samfilcon A were chosen as representatives of SiHy materials fabricated with an internal wetting agent. A confluent and dim HA–safranin network was observed adhered to balafilcon A, senofilcon A, and samfilcon A lens surfaces incubated with either 0.1% (w/v) HA solution or Biotrue MPS. Therefore, the conditioning function provided by Biotrue MPS may be in part explained by the presence of the HA humectant layer that readily sorbs on the various types of SiHy contact lens materials. Keywords: contact lens, hyaluronan, MPS, AFM, CLSM, DIC microscopyWygladacz KAHook DJDove Medical Pressarticlecontact lenshyaluronanMPSAFMCSLMDIC microscopyOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2016, Iss Issue 1, Pp 1423-1433 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	contact lens hyaluronan MPS AFM CSLM DIC microscopy Ophthalmology RE1-994
spellingShingle	contact lens hyaluronan MPS AFM CSLM DIC microscopy Ophthalmology RE1-994 Wygladacz KA Hook DJ Visualization of a hyaluronan network on the surface of silicone-hydrogel materials
description	Katarzyna A Wygladacz, Daniel J Hook Vision Care, Bausch & Lomb Incorporated, Rochester, NY, USA Abstract: Biotrue multipurpose solution (MPS) is a bioinspired disinfecting and conditioning solution that includes hyaluronic acid (HA) as a natural wetting agent. Previous studies demonstrated that HA sorbed from Biotrue MPS on both conventional and silicone hydrogel (SiHy) contact lens materials; an in vitro simulated-wear test validated the presence of HA on the lens surfaces for as long as 20 hours. In this study, the morphology and distribution of HA sorbed from both Biotrue and pure HA solution on SiHy contact lens surfaces was examined. Atomic force microscopy imaging was used to illustrate the topography of fresh SiHy contact lens materials before and after incubation with 0.1% (w/v) HA solution. The distribution, as well as fine details of the HA network, were resolved by first staining HA with Gram’s safranin, then imaging with confocal laser-scanning microscopy and differential interference-contrast microscopy. In this approach, SiHy materials take up the dye (safranin) nonspecifically, such that the resultant safranin–HA complex appears dim against the fluorescent lens background. Balafilcon A was chosen as the representative of glassy SiHy lenses that require postpolymerization plasma treatment to increase wettability. Senofilcon A and samfilcon A were chosen as representatives of SiHy materials fabricated with an internal wetting agent. A confluent and dim HA–safranin network was observed adhered to balafilcon A, senofilcon A, and samfilcon A lens surfaces incubated with either 0.1% (w/v) HA solution or Biotrue MPS. Therefore, the conditioning function provided by Biotrue MPS may be in part explained by the presence of the HA humectant layer that readily sorbs on the various types of SiHy contact lens materials. Keywords: contact lens, hyaluronan, MPS, AFM, CLSM, DIC microscopy
format	article
author	Wygladacz KA Hook DJ
author_facet	Wygladacz KA Hook DJ
author_sort	Wygladacz KA
title	Visualization of a hyaluronan network on the surface of silicone-hydrogel materials
title_short	Visualization of a hyaluronan network on the surface of silicone-hydrogel materials
title_full	Visualization of a hyaluronan network on the surface of silicone-hydrogel materials
title_fullStr	Visualization of a hyaluronan network on the surface of silicone-hydrogel materials
title_full_unstemmed	Visualization of a hyaluronan network on the surface of silicone-hydrogel materials
title_sort	visualization of a hyaluronan network on the surface of silicone-hydrogel materials
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/52b6af1f879045f48e25a30a0edbfa9e
work_keys_str_mv	AT wygladaczka visualizationofahyaluronannetworkonthesurfaceofsiliconehydrogelmaterials AT hookdj visualizationofahyaluronannetworkonthesurfaceofsiliconehydrogelmaterials
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Visualization of a hyaluronan network on the surface of silicone-hydrogel materials

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