Enhanced Resistance of atbzip62 against Pseudomonas syringae pv. tomato Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by AtbZIP62 Transcription Factor

The intrinsic defense mechanisms of plants toward pathogenic bacteria have been widely investigated for years and are still at the center of interest in plant biosciences research. This study investigated the role of the AtbZIP62 gene encoding a transcription factor (TF) in the ba...

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Autores principales:	Rizwana Begum Syed Nabi, Nkulu Kabange Rolly, Rupesh Tayade, Murtaza Khan, Muhammad Shahid, Byung-Wook Yun
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	disease resistance <i>Pseudomonas syringae</i> pv. <i>tomato</i> <i>AtbZIP62</i> TF systemic acquired resistance <i>Arabidopsis</i> Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/52b8c62d79714a629579d0bc6ac126b7
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spelling	oai:doaj.org-article:52b8c62d79714a629579d0bc6ac126b72021-11-11T17:00:28ZEnhanced Resistance of <i>atbzip62</i> against <i>Pseudomonas syringae</i> pv. <i>tomato</i> Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by <i>AtbZIP62</i> Transcription Factor10.3390/ijms2221115411422-00671661-6596https://doaj.org/article/52b8c62d79714a629579d0bc6ac126b72021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11541https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The intrinsic defense mechanisms of plants toward pathogenic bacteria have been widely investigated for years and are still at the center of interest in plant biosciences research. This study investigated the role of the <i>AtbZIP62</i> gene encoding a transcription factor (TF) in the basal defense and systemic acquired resistance in Arabidopsis using the reverse genetics approach. To achieve that, the <i>atbzip62</i> mutant line (lacking the <i>AtbZIP62</i> gene) was challenged with <i>Pseudomonas syringae</i> pv. <i>tomato</i> (<i>Pst DC3000</i>) inoculated by infiltration into Arabidopsis leaves at the rosette stage. The results indicated that <i>atbzip62</i> plants showed an enhanced resistance phenotype toward <i>Pst DC3000 vir</i> over time compared to Col-0 and the susceptible disease controls, <i>atgsnor1-3</i> and <i>atsid2.</i> In addition, the transcript accumulation of pathogenesis-related genes, <i>AtPR1</i> and <i>AtPR2</i>, increased significantly in <i>atbzip62</i> over time (0–72 h post-inoculation, hpi) compared to that of <i>atgsnor1-3</i> and <i>atsid2</i> (susceptible lines), with <i>AtPR1</i> prevailing over <i>AtPR2</i>. When coupled with the recorded pathogen growth (expressed as a colony-forming unit, CFU mL<sup>−1</sup>), the induction of <i>PR</i> genes, associated with the salicylic acid (SA) defense signaling, in part explained the observed enhanced resistance of <i>atbzip62</i> mutant plants in response to <i>Pst DC3000 vir</i>. Furthermore, when <i>Pst DC3000 avrB</i> was inoculated, the expression of <i>AtPR1</i> was upregulated in the systemic leaves of Col-0, while that of <i>AtPR2</i> remained at a basal level in Col-0. Moreover, the expression of <i>AtAZI</i> (a systemic acquired resistance -related) gene was significantly upregulated at all time points (0–24 h post-inoculation, hpi) in <i>atbzip62</i> compared to Col-0 and <i>atgsnor1-3</i> and <i>atsid2</i>. Under the same conditions, <i>AtG3DPH</i> exhibited a high transcript accumulation level 48 hpi in the <i>atbzip62</i> background. Therefore, all data put together suggest that <i>AtPR1</i> and <i>AtPR2</i> coupled with <i>AtAZI</i> and <i>AtG3DPH</i>, with <i>AtAZI</i> prevailing over <i>AtG3DPH</i>, would contribute to the recorded enhanced resistance phenotype of the <i>atbzip62</i> mutant line against <i>Pst DC3000</i>. Thus, the <i>AtbZIP62</i> TF is proposed as a negative regulator of basal defense and systemic acquired resistance in plants under <i>Pst DC3000</i> infection.Rizwana Begum Syed NabiNkulu Kabange RollyRupesh TayadeMurtaza KhanMuhammad ShahidByung-Wook YunMDPI AGarticledisease resistance<i>Pseudomonas syringae</i> pv. <i>tomato</i><i>AtbZIP62</i> TFsystemic acquired resistance<i>Arabidopsis</i>Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11541, p 11541 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	disease resistance <i>Pseudomonas syringae</i> pv. <i>tomato</i> <i>AtbZIP62</i> TF systemic acquired resistance <i>Arabidopsis</i> Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	disease resistance <i>Pseudomonas syringae</i> pv. <i>tomato</i> <i>AtbZIP62</i> TF systemic acquired resistance <i>Arabidopsis</i> Biology (General) QH301-705.5 Chemistry QD1-999 Rizwana Begum Syed Nabi Nkulu Kabange Rolly Rupesh Tayade Murtaza Khan Muhammad Shahid Byung-Wook Yun Enhanced Resistance of <i>atbzip62</i> against <i>Pseudomonas syringae</i> pv. <i>tomato</i> Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by <i>AtbZIP62</i> Transcription Factor
description	The intrinsic defense mechanisms of plants toward pathogenic bacteria have been widely investigated for years and are still at the center of interest in plant biosciences research. This study investigated the role of the <i>AtbZIP62</i> gene encoding a transcription factor (TF) in the basal defense and systemic acquired resistance in Arabidopsis using the reverse genetics approach. To achieve that, the <i>atbzip62</i> mutant line (lacking the <i>AtbZIP62</i> gene) was challenged with <i>Pseudomonas syringae</i> pv. <i>tomato</i> (<i>Pst DC3000</i>) inoculated by infiltration into Arabidopsis leaves at the rosette stage. The results indicated that <i>atbzip62</i> plants showed an enhanced resistance phenotype toward <i>Pst DC3000 vir</i> over time compared to Col-0 and the susceptible disease controls, <i>atgsnor1-3</i> and <i>atsid2.</i> In addition, the transcript accumulation of pathogenesis-related genes, <i>AtPR1</i> and <i>AtPR2</i>, increased significantly in <i>atbzip62</i> over time (0–72 h post-inoculation, hpi) compared to that of <i>atgsnor1-3</i> and <i>atsid2</i> (susceptible lines), with <i>AtPR1</i> prevailing over <i>AtPR2</i>. When coupled with the recorded pathogen growth (expressed as a colony-forming unit, CFU mL<sup>−1</sup>), the induction of <i>PR</i> genes, associated with the salicylic acid (SA) defense signaling, in part explained the observed enhanced resistance of <i>atbzip62</i> mutant plants in response to <i>Pst DC3000 vir</i>. Furthermore, when <i>Pst DC3000 avrB</i> was inoculated, the expression of <i>AtPR1</i> was upregulated in the systemic leaves of Col-0, while that of <i>AtPR2</i> remained at a basal level in Col-0. Moreover, the expression of <i>AtAZI</i> (a systemic acquired resistance -related) gene was significantly upregulated at all time points (0–24 h post-inoculation, hpi) in <i>atbzip62</i> compared to Col-0 and <i>atgsnor1-3</i> and <i>atsid2</i>. Under the same conditions, <i>AtG3DPH</i> exhibited a high transcript accumulation level 48 hpi in the <i>atbzip62</i> background. Therefore, all data put together suggest that <i>AtPR1</i> and <i>AtPR2</i> coupled with <i>AtAZI</i> and <i>AtG3DPH</i>, with <i>AtAZI</i> prevailing over <i>AtG3DPH</i>, would contribute to the recorded enhanced resistance phenotype of the <i>atbzip62</i> mutant line against <i>Pst DC3000</i>. Thus, the <i>AtbZIP62</i> TF is proposed as a negative regulator of basal defense and systemic acquired resistance in plants under <i>Pst DC3000</i> infection.
format	article
author	Rizwana Begum Syed Nabi Nkulu Kabange Rolly Rupesh Tayade Murtaza Khan Muhammad Shahid Byung-Wook Yun
author_facet	Rizwana Begum Syed Nabi Nkulu Kabange Rolly Rupesh Tayade Murtaza Khan Muhammad Shahid Byung-Wook Yun
author_sort	Rizwana Begum Syed Nabi
title	Enhanced Resistance of <i>atbzip62</i> against <i>Pseudomonas syringae</i> pv. <i>tomato</i> Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by <i>AtbZIP62</i> Transcription Factor
title_short	Enhanced Resistance of <i>atbzip62</i> against <i>Pseudomonas syringae</i> pv. <i>tomato</i> Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by <i>AtbZIP62</i> Transcription Factor
title_full	Enhanced Resistance of <i>atbzip62</i> against <i>Pseudomonas syringae</i> pv. <i>tomato</i> Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by <i>AtbZIP62</i> Transcription Factor
title_fullStr	Enhanced Resistance of <i>atbzip62</i> against <i>Pseudomonas syringae</i> pv. <i>tomato</i> Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by <i>AtbZIP62</i> Transcription Factor
title_full_unstemmed	Enhanced Resistance of <i>atbzip62</i> against <i>Pseudomonas syringae</i> pv. <i>tomato</i> Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by <i>AtbZIP62</i> Transcription Factor
title_sort	enhanced resistance of <i>atbzip62</i> against <i>pseudomonas syringae</i> pv. <i>tomato</i> suggests negative regulation of plant basal defense and systemic acquired resistance by <i>atbzip62</i> transcription factor
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/52b8c62d79714a629579d0bc6ac126b7
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Enhanced Resistance of <i>atbzip62</i> against <i>Pseudomonas syringae</i> pv. <i>tomato</i> Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by <i>AtbZIP62</i> Transcription Factor

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