Lack of gamma delta T cells ameliorates inflammatory response after acute intestinal ischemia reperfusion in mice

Abstract T-cells have been demonstrated to modulate ischemia–reperfusion injury (IRI) in the kidney, lung, liver, and intestine. Whereas most T-cell subpopulations contribute primarily to the antigen-specific effector and memory phases of immunity, γδ-T-cells combine adaptive features with rapid, in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dominik Funken, Yi Yu, Xiaoyan Feng, Tawan Imvised, Faikah Gueler, Immo Prinz, Omid Madadi-Sanjani, Benno M. Ure, Jochen F. Kuebler, Christian Klemann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/52b8cb0e1cb9406eaf61fb5dfebc1513
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:52b8cb0e1cb9406eaf61fb5dfebc1513
record_format	dspace
spelling	oai:doaj.org-article:52b8cb0e1cb9406eaf61fb5dfebc15132021-12-02T17:27:03ZLack of gamma delta T cells ameliorates inflammatory response after acute intestinal ischemia reperfusion in mice10.1038/s41598-021-96525-y2045-2322https://doaj.org/article/52b8cb0e1cb9406eaf61fb5dfebc15132021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96525-yhttps://doaj.org/toc/2045-2322Abstract T-cells have been demonstrated to modulate ischemia–reperfusion injury (IRI) in the kidney, lung, liver, and intestine. Whereas most T-cell subpopulations contribute primarily to the antigen-specific effector and memory phases of immunity, γδ-T-cells combine adaptive features with rapid, innate-like responses that can place them in the initiation phase of immune reactions. Therefore, we aimed to clarify the role of γδ-T-cells in intestinal IRI. Adult wild-type (WT) and γδ-T-cell-deficient mice were subjected to acute intestinal IRI. Gene expression of pro-inflammatory cytokines and influx of leukocyte subpopulations in the gut were assessed by qPCR and flow cytometry. Serum transaminases were measured as an indicator of distant organ IRI. Intestinal IRI led to increased influx of neutrophils, pro-inflammatory cytokine expression and LDH/ALT/AST elevation. Selective deficiency of γδ-T-cells significantly decreased pro-inflammatory cytokine levels and neutrophil infiltration in the gut following IRI compared to controls. Furthermore, γδ-T-cell deficiency resulted in decreased LDH and transaminases levels in sera, indicating amelioration of distant organ injury. Increasing evidence demonstrates a key role of T-cell subpopulations in IRI. We demonstrate that γδ-T-cell deficiency ameliorated pro-inflammatory cytokine production, neutrophil recruitment and distant organ injury. Thus, γδ-T-cells may be considered as mediators contributing to the inflammatory response in the acute phase of intestinal IRI.Dominik FunkenYi YuXiaoyan FengTawan ImvisedFaikah GuelerImmo PrinzOmid Madadi-SanjaniBenno M. UreJochen F. KueblerChristian KlemannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dominik Funken Yi Yu Xiaoyan Feng Tawan Imvised Faikah Gueler Immo Prinz Omid Madadi-Sanjani Benno M. Ure Jochen F. Kuebler Christian Klemann Lack of gamma delta T cells ameliorates inflammatory response after acute intestinal ischemia reperfusion in mice
description	Abstract T-cells have been demonstrated to modulate ischemia–reperfusion injury (IRI) in the kidney, lung, liver, and intestine. Whereas most T-cell subpopulations contribute primarily to the antigen-specific effector and memory phases of immunity, γδ-T-cells combine adaptive features with rapid, innate-like responses that can place them in the initiation phase of immune reactions. Therefore, we aimed to clarify the role of γδ-T-cells in intestinal IRI. Adult wild-type (WT) and γδ-T-cell-deficient mice were subjected to acute intestinal IRI. Gene expression of pro-inflammatory cytokines and influx of leukocyte subpopulations in the gut were assessed by qPCR and flow cytometry. Serum transaminases were measured as an indicator of distant organ IRI. Intestinal IRI led to increased influx of neutrophils, pro-inflammatory cytokine expression and LDH/ALT/AST elevation. Selective deficiency of γδ-T-cells significantly decreased pro-inflammatory cytokine levels and neutrophil infiltration in the gut following IRI compared to controls. Furthermore, γδ-T-cell deficiency resulted in decreased LDH and transaminases levels in sera, indicating amelioration of distant organ injury. Increasing evidence demonstrates a key role of T-cell subpopulations in IRI. We demonstrate that γδ-T-cell deficiency ameliorated pro-inflammatory cytokine production, neutrophil recruitment and distant organ injury. Thus, γδ-T-cells may be considered as mediators contributing to the inflammatory response in the acute phase of intestinal IRI.
format	article
author	Dominik Funken Yi Yu Xiaoyan Feng Tawan Imvised Faikah Gueler Immo Prinz Omid Madadi-Sanjani Benno M. Ure Jochen F. Kuebler Christian Klemann
author_facet	Dominik Funken Yi Yu Xiaoyan Feng Tawan Imvised Faikah Gueler Immo Prinz Omid Madadi-Sanjani Benno M. Ure Jochen F. Kuebler Christian Klemann
author_sort	Dominik Funken
title	Lack of gamma delta T cells ameliorates inflammatory response after acute intestinal ischemia reperfusion in mice
title_short	Lack of gamma delta T cells ameliorates inflammatory response after acute intestinal ischemia reperfusion in mice
title_full	Lack of gamma delta T cells ameliorates inflammatory response after acute intestinal ischemia reperfusion in mice
title_fullStr	Lack of gamma delta T cells ameliorates inflammatory response after acute intestinal ischemia reperfusion in mice
title_full_unstemmed	Lack of gamma delta T cells ameliorates inflammatory response after acute intestinal ischemia reperfusion in mice
title_sort	lack of gamma delta t cells ameliorates inflammatory response after acute intestinal ischemia reperfusion in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/52b8cb0e1cb9406eaf61fb5dfebc1513
work_keys_str_mv	AT dominikfunken lackofgammadeltatcellsamelioratesinflammatoryresponseafteracuteintestinalischemiareperfusioninmice AT yiyu lackofgammadeltatcellsamelioratesinflammatoryresponseafteracuteintestinalischemiareperfusioninmice AT xiaoyanfeng lackofgammadeltatcellsamelioratesinflammatoryresponseafteracuteintestinalischemiareperfusioninmice AT tawanimvised lackofgammadeltatcellsamelioratesinflammatoryresponseafteracuteintestinalischemiareperfusioninmice AT faikahgueler lackofgammadeltatcellsamelioratesinflammatoryresponseafteracuteintestinalischemiareperfusioninmice AT immoprinz lackofgammadeltatcellsamelioratesinflammatoryresponseafteracuteintestinalischemiareperfusioninmice AT omidmadadisanjani lackofgammadeltatcellsamelioratesinflammatoryresponseafteracuteintestinalischemiareperfusioninmice AT bennomure lackofgammadeltatcellsamelioratesinflammatoryresponseafteracuteintestinalischemiareperfusioninmice AT jochenfkuebler lackofgammadeltatcellsamelioratesinflammatoryresponseafteracuteintestinalischemiareperfusioninmice AT christianklemann lackofgammadeltatcellsamelioratesinflammatoryresponseafteracuteintestinalischemiareperfusioninmice
_version_	1718380787592593408

Lack of gamma delta T cells ameliorates inflammatory response after acute intestinal ischemia reperfusion in mice

Ejemplares similares