Nested PCR methods for detection Toxoplasma gondii B1 gene in Cerebrospinal Fluid of HIV patients

Background: Toxoplasmosis is a disease caused by infection of Toxoplasma gondii, Which may cause a life-threatening condition in immunocompromised patients, for example, Toxoplasma encephalitis (TE). It is challenging to diagnose Toxoplasma as a cause of central nervous system (CNS) infection in HIV...

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Autores principales:	Ryan Halleyantoro, Yunilda Andriyani, Ika Puspa Sari, Agnes Kurniawan
Formato:	article
Lenguaje:	EN
Publicado:	Diponegoro University 2019
Materias:	cerebrospinal fluid toxoplasma gondii pcr igg anti toxoplasma hiv Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/52bea462281741a9a4237654bf22aaa2
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spelling	oai:doaj.org-article:52bea462281741a9a4237654bf22aaa22021-11-05T16:47:32ZNested PCR methods for detection Toxoplasma gondii B1 gene in Cerebrospinal Fluid of HIV patients2503-217810.14710/jbtr.v5i2.4840https://doaj.org/article/52bea462281741a9a4237654bf22aaa22019-12-01T00:00:00Zhttps://ejournal2.undip.ac.id/index.php/jbtr/article/view/4840https://doaj.org/toc/2503-2178Background: Toxoplasmosis is a disease caused by infection of Toxoplasma gondii, Which may cause a life-threatening condition in immunocompromised patients, for example, Toxoplasma encephalitis (TE). It is challenging to diagnose Toxoplasma as a cause of central nervous system (CNS) infection in HIV patient, so we need an alternative method, which is a PCR detection of Toxoplasma gondii B1 gene. Objective: This research aimed to find association between PCR methods for Toxoplasma gondii B1 gene and anti-Toxoplasma IgG from cerebral spinal fluid patient HIV AIDS. Methods: A cross-sectional study was conducted to Cerebrospinal fluid (CSF) samples of HIV patients with neurological symptoms to determine Toxoplasma gondii infection using nested PCR methods for the B1 gene and detection of anti-Toxoplasma IgG. Results: 88 CSF samples from HIV patients tested using nested PCR showed 23 samples (26,1%) were positive. Serologic test for IgG Toxoplasma showed 34 samples were positive (28,6%). There was a significant correlation (p=0.000(<0.05) between PCR result and a serologic test for IgG Toxoplasma. Conclusion: Nested PCR methods to detect B1 gene increased the accuracy of diagnosis for toxoplasma encephalitis.Ryan HalleyantoroYunilda AndriyaniIka Puspa SariAgnes KurniawanDiponegoro Universityarticlecerebrospinal fluidtoxoplasma gondiipcrigg anti toxoplasmahivMedicine (General)R5-920ENJournal of Biomedicine and Translational Research, Vol 5, Iss 2, Pp 62-66 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cerebrospinal fluid toxoplasma gondii pcr igg anti toxoplasma hiv Medicine (General) R5-920
spellingShingle	cerebrospinal fluid toxoplasma gondii pcr igg anti toxoplasma hiv Medicine (General) R5-920 Ryan Halleyantoro Yunilda Andriyani Ika Puspa Sari Agnes Kurniawan Nested PCR methods for detection Toxoplasma gondii B1 gene in Cerebrospinal Fluid of HIV patients
description	Background: Toxoplasmosis is a disease caused by infection of Toxoplasma gondii, Which may cause a life-threatening condition in immunocompromised patients, for example, Toxoplasma encephalitis (TE). It is challenging to diagnose Toxoplasma as a cause of central nervous system (CNS) infection in HIV patient, so we need an alternative method, which is a PCR detection of Toxoplasma gondii B1 gene. Objective: This research aimed to find association between PCR methods for Toxoplasma gondii B1 gene and anti-Toxoplasma IgG from cerebral spinal fluid patient HIV AIDS. Methods: A cross-sectional study was conducted to Cerebrospinal fluid (CSF) samples of HIV patients with neurological symptoms to determine Toxoplasma gondii infection using nested PCR methods for the B1 gene and detection of anti-Toxoplasma IgG. Results: 88 CSF samples from HIV patients tested using nested PCR showed 23 samples (26,1%) were positive. Serologic test for IgG Toxoplasma showed 34 samples were positive (28,6%). There was a significant correlation (p=0.000(<0.05) between PCR result and a serologic test for IgG Toxoplasma. Conclusion: Nested PCR methods to detect B1 gene increased the accuracy of diagnosis for toxoplasma encephalitis.
format	article
author	Ryan Halleyantoro Yunilda Andriyani Ika Puspa Sari Agnes Kurniawan
author_facet	Ryan Halleyantoro Yunilda Andriyani Ika Puspa Sari Agnes Kurniawan
author_sort	Ryan Halleyantoro
title	Nested PCR methods for detection Toxoplasma gondii B1 gene in Cerebrospinal Fluid of HIV patients
title_short	Nested PCR methods for detection Toxoplasma gondii B1 gene in Cerebrospinal Fluid of HIV patients
title_full	Nested PCR methods for detection Toxoplasma gondii B1 gene in Cerebrospinal Fluid of HIV patients
title_fullStr	Nested PCR methods for detection Toxoplasma gondii B1 gene in Cerebrospinal Fluid of HIV patients
title_full_unstemmed	Nested PCR methods for detection Toxoplasma gondii B1 gene in Cerebrospinal Fluid of HIV patients
title_sort	nested pcr methods for detection toxoplasma gondii b1 gene in cerebrospinal fluid of hiv patients
publisher	Diponegoro University
publishDate	2019
url	https://doaj.org/article/52bea462281741a9a4237654bf22aaa2
work_keys_str_mv	AT ryanhalleyantoro nestedpcrmethodsfordetectiontoxoplasmagondiib1geneincerebrospinalfluidofhivpatients AT yunildaandriyani nestedpcrmethodsfordetectiontoxoplasmagondiib1geneincerebrospinalfluidofhivpatients AT ikapuspasari nestedpcrmethodsfordetectiontoxoplasmagondiib1geneincerebrospinalfluidofhivpatients AT agneskurniawan nestedpcrmethodsfordetectiontoxoplasmagondiib1geneincerebrospinalfluidofhivpatients
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Nested PCR methods for detection Toxoplasma gondii B1 gene in Cerebrospinal Fluid of HIV patients

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