The impact of entropy on the spatial organization of synaptonemal complexes within the cell nucleus.

We employ 4Pi-microscopy to study SC organization in mouse spermatocyte nuclei allowing for the three-dimensional reconstruction of the SC's backbone arrangement. Additionally, we model the SCs in the cell nucleus by confined, self-avoiding polymers, whose chain ends are attached to the envelop...

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Autores principales:	Miriam Fritsche, Laura G Reinholdt, Mark Lessard, Mary Ann Handel, Jörg Bewersdorf, Dieter W Heermann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/52c58981f440402a916cf288e84bd4c3
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spelling	oai:doaj.org-article:52c58981f440402a916cf288e84bd4c32021-11-18T07:19:40ZThe impact of entropy on the spatial organization of synaptonemal complexes within the cell nucleus.1932-620310.1371/journal.pone.0036282https://doaj.org/article/52c58981f440402a916cf288e84bd4c32012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22574147/?tool=EBIhttps://doaj.org/toc/1932-6203We employ 4Pi-microscopy to study SC organization in mouse spermatocyte nuclei allowing for the three-dimensional reconstruction of the SC's backbone arrangement. Additionally, we model the SCs in the cell nucleus by confined, self-avoiding polymers, whose chain ends are attached to the envelope of the confining cavity and diffuse along it. This work helps to elucidate the role of entropy in shaping pachytene SC organization. The framework provided by the complex interplay between SC polymer rigidity, tethering and confinement is able to qualitatively explain features of SC organization, such as mean squared end-to-end distances, mean squared center-of-mass distances, or SC density distributions. However, it fails in correctly assessing SC entanglement within the nucleus. In fact, our analysis of the 4Pi-microscopy images reveals a higher ordering of SCs within the nuclear volume than what is expected by our numerical model. This suggests that while effects of entropy impact SC organization, the dedicated action of proteins or actin cables is required to fine-tune the spatial ordering of SCs within the cell nucleus.Miriam FritscheLaura G ReinholdtMark LessardMary Ann HandelJörg BewersdorfDieter W HeermannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36282 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miriam Fritsche Laura G Reinholdt Mark Lessard Mary Ann Handel Jörg Bewersdorf Dieter W Heermann The impact of entropy on the spatial organization of synaptonemal complexes within the cell nucleus.
description	We employ 4Pi-microscopy to study SC organization in mouse spermatocyte nuclei allowing for the three-dimensional reconstruction of the SC's backbone arrangement. Additionally, we model the SCs in the cell nucleus by confined, self-avoiding polymers, whose chain ends are attached to the envelope of the confining cavity and diffuse along it. This work helps to elucidate the role of entropy in shaping pachytene SC organization. The framework provided by the complex interplay between SC polymer rigidity, tethering and confinement is able to qualitatively explain features of SC organization, such as mean squared end-to-end distances, mean squared center-of-mass distances, or SC density distributions. However, it fails in correctly assessing SC entanglement within the nucleus. In fact, our analysis of the 4Pi-microscopy images reveals a higher ordering of SCs within the nuclear volume than what is expected by our numerical model. This suggests that while effects of entropy impact SC organization, the dedicated action of proteins or actin cables is required to fine-tune the spatial ordering of SCs within the cell nucleus.
format	article
author	Miriam Fritsche Laura G Reinholdt Mark Lessard Mary Ann Handel Jörg Bewersdorf Dieter W Heermann
author_facet	Miriam Fritsche Laura G Reinholdt Mark Lessard Mary Ann Handel Jörg Bewersdorf Dieter W Heermann
author_sort	Miriam Fritsche
title	The impact of entropy on the spatial organization of synaptonemal complexes within the cell nucleus.
title_short	The impact of entropy on the spatial organization of synaptonemal complexes within the cell nucleus.
title_full	The impact of entropy on the spatial organization of synaptonemal complexes within the cell nucleus.
title_fullStr	The impact of entropy on the spatial organization of synaptonemal complexes within the cell nucleus.
title_full_unstemmed	The impact of entropy on the spatial organization of synaptonemal complexes within the cell nucleus.
title_sort	impact of entropy on the spatial organization of synaptonemal complexes within the cell nucleus.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/52c58981f440402a916cf288e84bd4c3
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The impact of entropy on the spatial organization of synaptonemal complexes within the cell nucleus.

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