Initial characterisation of adult human ovarian cell populations isolated by DDX4 expression and aldehyde dehydrogenase activity

Abstract The existence of a population of putative stem cells with germline developmental potential (oogonial stem cells: OSCs) in the adult mammalian ovary has been marked by controversy over isolation methodology and potential for in-vitro transformation, particularly where cell sorting has been b...

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Autores principales: Yvonne L. Clarkson, Marie McLaughlin, Martin Waterfall, Cheryl E. Dunlop, Paul A. Skehel, Richard A. Anderson, Evelyn E. Telfer
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Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/52dccacc26814c61baeefdbc37202808
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spelling oai:doaj.org-article:52dccacc26814c61baeefdbc372028082021-12-02T11:40:54ZInitial characterisation of adult human ovarian cell populations isolated by DDX4 expression and aldehyde dehydrogenase activity10.1038/s41598-018-25116-12045-2322https://doaj.org/article/52dccacc26814c61baeefdbc372028082018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25116-1https://doaj.org/toc/2045-2322Abstract The existence of a population of putative stem cells with germline developmental potential (oogonial stem cells: OSCs) in the adult mammalian ovary has been marked by controversy over isolation methodology and potential for in-vitro transformation, particularly where cell sorting has been based on expression of DEAD box polypeptide 4 (DDX4). This study describes a refined tissue dissociation/fluorescence-activated cell sorting (FACS) protocol for the ovaries of adult women which results in increased cell viability and yield of putative OSCs. A FACS technique incorporating dual-detection of DDX4 with aldehyde dehydrogenase 1 (ALDH1) demonstrates the existence of two sub-populations of small DDX4-positive cells (approx. 7 µm diameter) with ALDH1 activity, distinguished by expression of differentially spliced DDX4 transcripts and of DAZL, a major regulator of germ cell differentiation. These may indicate stages of differentiation from a progenitor population and provide a likely explanation for the expression disparities reported previously. These findings provide a robust basis for the further characterisation of these cells, and exploration of their potential physiological roles and therapeutic application.Yvonne L. ClarksonMarie McLaughlinMartin WaterfallCheryl E. DunlopPaul A. SkehelRichard A. AndersonEvelyn E. TelferNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yvonne L. Clarkson
Marie McLaughlin
Martin Waterfall
Cheryl E. Dunlop
Paul A. Skehel
Richard A. Anderson
Evelyn E. Telfer
Initial characterisation of adult human ovarian cell populations isolated by DDX4 expression and aldehyde dehydrogenase activity
description Abstract The existence of a population of putative stem cells with germline developmental potential (oogonial stem cells: OSCs) in the adult mammalian ovary has been marked by controversy over isolation methodology and potential for in-vitro transformation, particularly where cell sorting has been based on expression of DEAD box polypeptide 4 (DDX4). This study describes a refined tissue dissociation/fluorescence-activated cell sorting (FACS) protocol for the ovaries of adult women which results in increased cell viability and yield of putative OSCs. A FACS technique incorporating dual-detection of DDX4 with aldehyde dehydrogenase 1 (ALDH1) demonstrates the existence of two sub-populations of small DDX4-positive cells (approx. 7 µm diameter) with ALDH1 activity, distinguished by expression of differentially spliced DDX4 transcripts and of DAZL, a major regulator of germ cell differentiation. These may indicate stages of differentiation from a progenitor population and provide a likely explanation for the expression disparities reported previously. These findings provide a robust basis for the further characterisation of these cells, and exploration of their potential physiological roles and therapeutic application.
format article
author Yvonne L. Clarkson
Marie McLaughlin
Martin Waterfall
Cheryl E. Dunlop
Paul A. Skehel
Richard A. Anderson
Evelyn E. Telfer
author_facet Yvonne L. Clarkson
Marie McLaughlin
Martin Waterfall
Cheryl E. Dunlop
Paul A. Skehel
Richard A. Anderson
Evelyn E. Telfer
author_sort Yvonne L. Clarkson
title Initial characterisation of adult human ovarian cell populations isolated by DDX4 expression and aldehyde dehydrogenase activity
title_short Initial characterisation of adult human ovarian cell populations isolated by DDX4 expression and aldehyde dehydrogenase activity
title_full Initial characterisation of adult human ovarian cell populations isolated by DDX4 expression and aldehyde dehydrogenase activity
title_fullStr Initial characterisation of adult human ovarian cell populations isolated by DDX4 expression and aldehyde dehydrogenase activity
title_full_unstemmed Initial characterisation of adult human ovarian cell populations isolated by DDX4 expression and aldehyde dehydrogenase activity
title_sort initial characterisation of adult human ovarian cell populations isolated by ddx4 expression and aldehyde dehydrogenase activity
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/52dccacc26814c61baeefdbc37202808
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