Sulfane Sulfur Is a Strong Inducer of the Multiple Antibiotic Resistance Regulator MarR in <i>Escherichia coli</i>

Sulfane sulfur, including persulfide and polysulfide, is produced from the metabolism of sulfur-containing organic compounds or from sulfide oxidation. It is a normal cellular component, participating in signaling. In bacteria, it modifies gene regulators to activate the expression of genes involved...

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Autores principales:	Huangwei Xu, Guanhua Xuan, Huaiwei Liu, Yongzhen Xia, Luying Xun
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sulfane sulfur signaling MarR DNA binding antibiotic resistance Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/52e23d59fba5463fb479b98ab4c70620
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spelling	oai:doaj.org-article:52e23d59fba5463fb479b98ab4c706202021-11-25T16:28:25ZSulfane Sulfur Is a Strong Inducer of the Multiple Antibiotic Resistance Regulator MarR in <i>Escherichia coli</i>10.3390/antiox101117782076-3921https://doaj.org/article/52e23d59fba5463fb479b98ab4c706202021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1778https://doaj.org/toc/2076-3921Sulfane sulfur, including persulfide and polysulfide, is produced from the metabolism of sulfur-containing organic compounds or from sulfide oxidation. It is a normal cellular component, participating in signaling. In bacteria, it modifies gene regulators to activate the expression of genes involved in sulfur metabolism. However, to determine whether sulfane sulfur is a common signal in bacteria, additional evidence is required. The ubiquitous multiple antibiotic resistance regulator (MarR) family of regulators controls the expression of numerous genes, but the intrinsic inducers are often elusive. Recently, two MarR family members, <i>Pseudomonas aeruginosa</i> MexR and <i>Staphylococcus aureus</i> MgrA, have been reported to sense sulfane sulfur. Here, we report that <i>Escherichia coli</i> MarR, the prototypical member of the family, also senses sulfane sulfur to form one or two disulfide or trisulfide bonds between two dimers. Although the tetramer with two disulfide bonds does not bind to its target DNA, our results suggest that the tetramer with one disulfide bond does bind to its target DNA, with reduced affinity. An MarR-repressed mKate reporter is strongly induced by polysulfide in <i>E. coli</i>. Further investigation is needed to determine whether sulfane sulfur is a common signal of the family members, but three members sense cellular sulfane sulfur to turn on antibiotic resistance genes. The findings offer additional support for a general signaling role of sulfane sulfur in bacteria.Huangwei XuGuanhua XuanHuaiwei LiuYongzhen XiaLuying XunMDPI AGarticlesulfane sulfursignalingMarRDNA bindingantibiotic resistanceTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1778, p 1778 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sulfane sulfur signaling MarR DNA binding antibiotic resistance Therapeutics. Pharmacology RM1-950
spellingShingle	sulfane sulfur signaling MarR DNA binding antibiotic resistance Therapeutics. Pharmacology RM1-950 Huangwei Xu Guanhua Xuan Huaiwei Liu Yongzhen Xia Luying Xun Sulfane Sulfur Is a Strong Inducer of the Multiple Antibiotic Resistance Regulator MarR in <i>Escherichia coli</i>
description	Sulfane sulfur, including persulfide and polysulfide, is produced from the metabolism of sulfur-containing organic compounds or from sulfide oxidation. It is a normal cellular component, participating in signaling. In bacteria, it modifies gene regulators to activate the expression of genes involved in sulfur metabolism. However, to determine whether sulfane sulfur is a common signal in bacteria, additional evidence is required. The ubiquitous multiple antibiotic resistance regulator (MarR) family of regulators controls the expression of numerous genes, but the intrinsic inducers are often elusive. Recently, two MarR family members, <i>Pseudomonas aeruginosa</i> MexR and <i>Staphylococcus aureus</i> MgrA, have been reported to sense sulfane sulfur. Here, we report that <i>Escherichia coli</i> MarR, the prototypical member of the family, also senses sulfane sulfur to form one or two disulfide or trisulfide bonds between two dimers. Although the tetramer with two disulfide bonds does not bind to its target DNA, our results suggest that the tetramer with one disulfide bond does bind to its target DNA, with reduced affinity. An MarR-repressed mKate reporter is strongly induced by polysulfide in <i>E. coli</i>. Further investigation is needed to determine whether sulfane sulfur is a common signal of the family members, but three members sense cellular sulfane sulfur to turn on antibiotic resistance genes. The findings offer additional support for a general signaling role of sulfane sulfur in bacteria.
format	article
author	Huangwei Xu Guanhua Xuan Huaiwei Liu Yongzhen Xia Luying Xun
author_facet	Huangwei Xu Guanhua Xuan Huaiwei Liu Yongzhen Xia Luying Xun
author_sort	Huangwei Xu
title	Sulfane Sulfur Is a Strong Inducer of the Multiple Antibiotic Resistance Regulator MarR in <i>Escherichia coli</i>
title_short	Sulfane Sulfur Is a Strong Inducer of the Multiple Antibiotic Resistance Regulator MarR in <i>Escherichia coli</i>
title_full	Sulfane Sulfur Is a Strong Inducer of the Multiple Antibiotic Resistance Regulator MarR in <i>Escherichia coli</i>
title_fullStr	Sulfane Sulfur Is a Strong Inducer of the Multiple Antibiotic Resistance Regulator MarR in <i>Escherichia coli</i>
title_full_unstemmed	Sulfane Sulfur Is a Strong Inducer of the Multiple Antibiotic Resistance Regulator MarR in <i>Escherichia coli</i>
title_sort	sulfane sulfur is a strong inducer of the multiple antibiotic resistance regulator marr in <i>escherichia coli</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/52e23d59fba5463fb479b98ab4c70620
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_version_	1718413135230009344

Sulfane Sulfur Is a Strong Inducer of the Multiple Antibiotic Resistance Regulator MarR in <i>Escherichia coli</i>

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