Panx1b Modulates the Luminance Response and Direction of Locomotion in the Zebrafish

Pannexin1 (Panx1) can form ATP-permeable channels that play roles in the physiology of the visual system. In the zebrafish two ohnologs of Panx1, Panx1a and Panx1b, have unique and shared channel properties and tissue expression patterns. Panx1a channels are located in horizontal cells of the outer...

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Autores principales:	Nickie Safarian, Sarah Houshangi-Tabrizi, Christiane Zoidl, Georg R. Zoidl
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pannexin1b zebrafish retinal ganglion cells Muller glia circadian clock visual system Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/52ed0b6f04334cf39a4dd44efbe0de5c
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spelling	oai:doaj.org-article:52ed0b6f04334cf39a4dd44efbe0de5c2021-11-11T17:12:28ZPanx1b Modulates the Luminance Response and Direction of Locomotion in the Zebrafish10.3390/ijms2221117501422-00671661-6596https://doaj.org/article/52ed0b6f04334cf39a4dd44efbe0de5c2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11750https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Pannexin1 (Panx1) can form ATP-permeable channels that play roles in the physiology of the visual system. In the zebrafish two ohnologs of Panx1, Panx1a and Panx1b, have unique and shared channel properties and tissue expression patterns. Panx1a channels are located in horizontal cells of the outer retina and modulate light decrement detection through an ATP/pH-dependent mechanisms and adenosine/dopamine signaling. Here, we decipher how the strategic localization of Panx1b channels in the inner retina and ganglion cell layer modulates visually evoked motor behavior. We describe a <i>panx1b</i> knockout model generated by TALEN technology. The RNA-seq analysis of 6 days post-fertilization larvae is confirmed by real-time PCR and paired with testing of locomotion behaviors by visual motor and optomotor response tests. We show that the loss of Panx1b channels disrupts the retinal response to an abrupt loss of illumination and it decreases the larval ability to follow leftward direction of locomotion in low light conditions. We concluded that the loss of Panx1b channels compromises the final output of luminance as well as motion detection. The Panx1b protein also emerges as a modulator of the circadian clock system. The disruption of the circadian clock system in mutants suggests that Panx1b could participate in non-image forming processes in the inner retina.Nickie SafarianSarah Houshangi-TabriziChristiane ZoidlGeorg R. ZoidlMDPI AGarticlepannexin1bzebrafishretinal ganglion cellsMuller gliacircadian clockvisual systemBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11750, p 11750 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pannexin1b zebrafish retinal ganglion cells Muller glia circadian clock visual system Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	pannexin1b zebrafish retinal ganglion cells Muller glia circadian clock visual system Biology (General) QH301-705.5 Chemistry QD1-999 Nickie Safarian Sarah Houshangi-Tabrizi Christiane Zoidl Georg R. Zoidl Panx1b Modulates the Luminance Response and Direction of Locomotion in the Zebrafish
description	Pannexin1 (Panx1) can form ATP-permeable channels that play roles in the physiology of the visual system. In the zebrafish two ohnologs of Panx1, Panx1a and Panx1b, have unique and shared channel properties and tissue expression patterns. Panx1a channels are located in horizontal cells of the outer retina and modulate light decrement detection through an ATP/pH-dependent mechanisms and adenosine/dopamine signaling. Here, we decipher how the strategic localization of Panx1b channels in the inner retina and ganglion cell layer modulates visually evoked motor behavior. We describe a <i>panx1b</i> knockout model generated by TALEN technology. The RNA-seq analysis of 6 days post-fertilization larvae is confirmed by real-time PCR and paired with testing of locomotion behaviors by visual motor and optomotor response tests. We show that the loss of Panx1b channels disrupts the retinal response to an abrupt loss of illumination and it decreases the larval ability to follow leftward direction of locomotion in low light conditions. We concluded that the loss of Panx1b channels compromises the final output of luminance as well as motion detection. The Panx1b protein also emerges as a modulator of the circadian clock system. The disruption of the circadian clock system in mutants suggests that Panx1b could participate in non-image forming processes in the inner retina.
format	article
author	Nickie Safarian Sarah Houshangi-Tabrizi Christiane Zoidl Georg R. Zoidl
author_facet	Nickie Safarian Sarah Houshangi-Tabrizi Christiane Zoidl Georg R. Zoidl
author_sort	Nickie Safarian
title	Panx1b Modulates the Luminance Response and Direction of Locomotion in the Zebrafish
title_short	Panx1b Modulates the Luminance Response and Direction of Locomotion in the Zebrafish
title_full	Panx1b Modulates the Luminance Response and Direction of Locomotion in the Zebrafish
title_fullStr	Panx1b Modulates the Luminance Response and Direction of Locomotion in the Zebrafish
title_full_unstemmed	Panx1b Modulates the Luminance Response and Direction of Locomotion in the Zebrafish
title_sort	panx1b modulates the luminance response and direction of locomotion in the zebrafish
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/52ed0b6f04334cf39a4dd44efbe0de5c
work_keys_str_mv	AT nickiesafarian panx1bmodulatestheluminanceresponseanddirectionoflocomotioninthezebrafish AT sarahhoushangitabrizi panx1bmodulatestheluminanceresponseanddirectionoflocomotioninthezebrafish AT christianezoidl panx1bmodulatestheluminanceresponseanddirectionoflocomotioninthezebrafish AT georgrzoidl panx1bmodulatestheluminanceresponseanddirectionoflocomotioninthezebrafish
_version_	1718432115866992640

Panx1b Modulates the Luminance Response and Direction of Locomotion in the Zebrafish

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