Tissue block staining and domestic adhesive tape yield qualified integral sections of adult mouse orbits and eyeballs.

The standard histological processing procedure, which produces excellent staining of sections for most tissues, fails to yield satisfactory results in adult mouse orbits or eyeballs. Here, we show that a protocol using tissue block staining and domestic adhesive tapes resulted in qualified integral...

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Autores principales:	Zhongmin Li, Martin Ungerer, Julia Faßbender, Clara Wenhart, Hans-Peter Holthoff, Goetz Muench
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5304dde8be454d9885fc4f0c99c3af87
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spelling	oai:doaj.org-article:5304dde8be454d9885fc4f0c99c3af872021-12-02T20:18:45ZTissue block staining and domestic adhesive tape yield qualified integral sections of adult mouse orbits and eyeballs.1932-620310.1371/journal.pone.0255363https://doaj.org/article/5304dde8be454d9885fc4f0c99c3af872021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255363https://doaj.org/toc/1932-6203The standard histological processing procedure, which produces excellent staining of sections for most tissues, fails to yield satisfactory results in adult mouse orbits or eyeballs. Here, we show that a protocol using tissue block staining and domestic adhesive tapes resulted in qualified integral serial cryo-sections of whole orbits or eyeballs, and the fine structures were well preserved. The histological processing protocol comprises paraformaldehyde fixation, ethylenediaminetetraacetic acid decalcification, tissue block staining with hematoxylin and eosin, embedding, adhesive tape aided sectioning, and water-soluble mounting. This protocol was proved to be the best in comparison with seven other related existing histological traditional or non-traditional processing methods, according to the staining slice quality. We observed a hundred percent success rate in sectioning, collection, and mounting with this method. The reproducibility tested on qualified section success rates and slice quality scores confirmed that the technique is reliable. The feasibility of the method to detect target molecules in orbits was verified by successful trial tests on block immunostaining and adhesive tape-aided sectioning. Application of this protocol in joints, brains, and so on,-the challenging integral sectioning tissues, also generated high-quality histological staining sections.Zhongmin LiMartin UngererJulia FaßbenderClara WenhartHans-Peter HolthoffGoetz MuenchPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255363 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhongmin Li Martin Ungerer Julia Faßbender Clara Wenhart Hans-Peter Holthoff Goetz Muench Tissue block staining and domestic adhesive tape yield qualified integral sections of adult mouse orbits and eyeballs.
description	The standard histological processing procedure, which produces excellent staining of sections for most tissues, fails to yield satisfactory results in adult mouse orbits or eyeballs. Here, we show that a protocol using tissue block staining and domestic adhesive tapes resulted in qualified integral serial cryo-sections of whole orbits or eyeballs, and the fine structures were well preserved. The histological processing protocol comprises paraformaldehyde fixation, ethylenediaminetetraacetic acid decalcification, tissue block staining with hematoxylin and eosin, embedding, adhesive tape aided sectioning, and water-soluble mounting. This protocol was proved to be the best in comparison with seven other related existing histological traditional or non-traditional processing methods, according to the staining slice quality. We observed a hundred percent success rate in sectioning, collection, and mounting with this method. The reproducibility tested on qualified section success rates and slice quality scores confirmed that the technique is reliable. The feasibility of the method to detect target molecules in orbits was verified by successful trial tests on block immunostaining and adhesive tape-aided sectioning. Application of this protocol in joints, brains, and so on,-the challenging integral sectioning tissues, also generated high-quality histological staining sections.
format	article
author	Zhongmin Li Martin Ungerer Julia Faßbender Clara Wenhart Hans-Peter Holthoff Goetz Muench
author_facet	Zhongmin Li Martin Ungerer Julia Faßbender Clara Wenhart Hans-Peter Holthoff Goetz Muench
author_sort	Zhongmin Li
title	Tissue block staining and domestic adhesive tape yield qualified integral sections of adult mouse orbits and eyeballs.
title_short	Tissue block staining and domestic adhesive tape yield qualified integral sections of adult mouse orbits and eyeballs.
title_full	Tissue block staining and domestic adhesive tape yield qualified integral sections of adult mouse orbits and eyeballs.
title_fullStr	Tissue block staining and domestic adhesive tape yield qualified integral sections of adult mouse orbits and eyeballs.
title_full_unstemmed	Tissue block staining and domestic adhesive tape yield qualified integral sections of adult mouse orbits and eyeballs.
title_sort	tissue block staining and domestic adhesive tape yield qualified integral sections of adult mouse orbits and eyeballs.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5304dde8be454d9885fc4f0c99c3af87
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Tissue block staining and domestic adhesive tape yield qualified integral sections of adult mouse orbits and eyeballs.

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