UvrY is required for the full virulence of Aeromonas dhakensis

Aeromonas dhakensis is an emerging human pathogen which causes fast and severe infections worldwide. Under the gradual pressure of lacking useful antibiotics, finding a new strategy against A. dhakensis infection is urgent. To understand its pathogenesis, we created an A. dhakensis AAK1 mini-Tn10 tr...

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Autores principales:	Yi-Wei Chen, Wen-Hsuan Yeh, Hung-Jen Tang, Jenn-Wei Chen, Hung-Yu Shu, Yu-Chen Su, Sin-Tian Wang, Cheng-Ju Kuo, Yin-Ching Chuang, Chi-Chung Chen, Wen-Chien Ko, Chang-Shi Chen, Po-Lin Chen
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	aeromonas dhakensis two-component system uvry pore-forming toxin hemolysin caenorhabditis elegans Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/530cc0ee246f45fe84486ff8979bdc5d
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spelling	oai:doaj.org-article:530cc0ee246f45fe84486ff8979bdc5d2021-11-17T14:21:58ZUvrY is required for the full virulence of Aeromonas dhakensis2150-55942150-560810.1080/21505594.2020.1768339https://doaj.org/article/530cc0ee246f45fe84486ff8979bdc5d2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1768339https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Aeromonas dhakensis is an emerging human pathogen which causes fast and severe infections worldwide. Under the gradual pressure of lacking useful antibiotics, finding a new strategy against A. dhakensis infection is urgent. To understand its pathogenesis, we created an A. dhakensis AAK1 mini-Tn10 transposon library to study the mechanism of A. dhakensis infection. By using a Caenorhabditis elegans model, we established a screening platform for the purpose of identifying attenuated mutants. The uvrY mutant, which conferred the most attenuated toxicity toward C. elegans, was identified. The uvrY mutant was also less virulent in C2C12 fibroblast and mice models, in line with in vitro results. To further elucidate the mechanism of UvrY in controlling the toxicity in A. dhakensis, we conducted a transcriptomic analysis. The RNAseq results showed that the expression of a unique hemolysin ahh1 and other virulence factors were regulated by UvrY. Complementation of Ahh1, one of the most important virulence factors, rescued the pore-formation phenotype of uvrY mutant in C. elegans; however, complementation of ahh1 endogenous promoter-driven ahh1 could not produce Ahh1 and rescue the virulence in the uvrY mutant. These findings suggest that UvrY is required for the expression of Ahh1 in A. dhakensis. Taken together, our results suggested that UvrY controls several different virulence factors and is required for the full virulence of A. dhakensis. The two-component regulator UvrY therefore a potential therapeutic target which is worthy of further study.Yi-Wei ChenWen-Hsuan YehHung-Jen TangJenn-Wei ChenHung-Yu ShuYu-Chen SuSin-Tian WangCheng-Ju KuoYin-Ching ChuangChi-Chung ChenWen-Chien KoChang-Shi ChenPo-Lin ChenTaylor & Francis Grouparticleaeromonas dhakensistwo-component systemuvrypore-forming toxinhemolysincaenorhabditis elegansInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 502-520 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	aeromonas dhakensis two-component system uvry pore-forming toxin hemolysin caenorhabditis elegans Infectious and parasitic diseases RC109-216
spellingShingle	aeromonas dhakensis two-component system uvry pore-forming toxin hemolysin caenorhabditis elegans Infectious and parasitic diseases RC109-216 Yi-Wei Chen Wen-Hsuan Yeh Hung-Jen Tang Jenn-Wei Chen Hung-Yu Shu Yu-Chen Su Sin-Tian Wang Cheng-Ju Kuo Yin-Ching Chuang Chi-Chung Chen Wen-Chien Ko Chang-Shi Chen Po-Lin Chen UvrY is required for the full virulence of Aeromonas dhakensis
description	Aeromonas dhakensis is an emerging human pathogen which causes fast and severe infections worldwide. Under the gradual pressure of lacking useful antibiotics, finding a new strategy against A. dhakensis infection is urgent. To understand its pathogenesis, we created an A. dhakensis AAK1 mini-Tn10 transposon library to study the mechanism of A. dhakensis infection. By using a Caenorhabditis elegans model, we established a screening platform for the purpose of identifying attenuated mutants. The uvrY mutant, which conferred the most attenuated toxicity toward C. elegans, was identified. The uvrY mutant was also less virulent in C2C12 fibroblast and mice models, in line with in vitro results. To further elucidate the mechanism of UvrY in controlling the toxicity in A. dhakensis, we conducted a transcriptomic analysis. The RNAseq results showed that the expression of a unique hemolysin ahh1 and other virulence factors were regulated by UvrY. Complementation of Ahh1, one of the most important virulence factors, rescued the pore-formation phenotype of uvrY mutant in C. elegans; however, complementation of ahh1 endogenous promoter-driven ahh1 could not produce Ahh1 and rescue the virulence in the uvrY mutant. These findings suggest that UvrY is required for the expression of Ahh1 in A. dhakensis. Taken together, our results suggested that UvrY controls several different virulence factors and is required for the full virulence of A. dhakensis. The two-component regulator UvrY therefore a potential therapeutic target which is worthy of further study.
format	article
author	Yi-Wei Chen Wen-Hsuan Yeh Hung-Jen Tang Jenn-Wei Chen Hung-Yu Shu Yu-Chen Su Sin-Tian Wang Cheng-Ju Kuo Yin-Ching Chuang Chi-Chung Chen Wen-Chien Ko Chang-Shi Chen Po-Lin Chen
author_facet	Yi-Wei Chen Wen-Hsuan Yeh Hung-Jen Tang Jenn-Wei Chen Hung-Yu Shu Yu-Chen Su Sin-Tian Wang Cheng-Ju Kuo Yin-Ching Chuang Chi-Chung Chen Wen-Chien Ko Chang-Shi Chen Po-Lin Chen
author_sort	Yi-Wei Chen
title	UvrY is required for the full virulence of Aeromonas dhakensis
title_short	UvrY is required for the full virulence of Aeromonas dhakensis
title_full	UvrY is required for the full virulence of Aeromonas dhakensis
title_fullStr	UvrY is required for the full virulence of Aeromonas dhakensis
title_full_unstemmed	UvrY is required for the full virulence of Aeromonas dhakensis
title_sort	uvry is required for the full virulence of aeromonas dhakensis
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/530cc0ee246f45fe84486ff8979bdc5d
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UvrY is required for the full virulence of Aeromonas dhakensis

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