Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm

Abstract Modification of outer membrane proteins (OMPs) is the first line of Gram-negative bacteria defence against antimicrobials. Here we point to Proteus mirabilis OMPs and their role in antibiotic and phage resistance. Protein profiles of amikacin (AMKrsv), phage (Brsv) and amikacin/phage (AMK/B...

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Autores principales:	Agnieszka Maszewska, Magdalena Moryl, Junli Wu, Bin Liu, Lu Feng, Antoni Rozalski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/531501436d654d0096ac7172dcb57f17
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spelling	oai:doaj.org-article:531501436d654d0096ac7172dcb57f172021-12-02T14:01:36ZAmikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm10.1038/s41598-020-80907-92045-2322https://doaj.org/article/531501436d654d0096ac7172dcb57f172021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80907-9https://doaj.org/toc/2045-2322Abstract Modification of outer membrane proteins (OMPs) is the first line of Gram-negative bacteria defence against antimicrobials. Here we point to Proteus mirabilis OMPs and their role in antibiotic and phage resistance. Protein profiles of amikacin (AMKrsv), phage (Brsv) and amikacin/phage (AMK/Brsv) resistant variants of P. mirabilis were compared to that obtained for a wild strain. In resistant variants there were identified 14, 1, 5 overexpressed and 13, 5, 1 downregulated proteins for AMKrsv, Brsv and AMK/Brsv, respectively. Application of phages with amikacin led to reducing the number of up- and downregulated proteins compared to single antibiotic treatment. Proteins isolated in AMKrsv are involved in protein biosynthesis, transcription and signal transduction, which correspond to well-known mechanisms of bacteria resistance to aminoglycosides. In isolated OMPs several cytoplasmic proteins, important in antibiotic resistance, were identified, probably as a result of environmental stress, e.g. elongation factor Tu, asparaginyl-tRNA and aspartyl-tRNA synthetases. In Brsv there were identified: NusA and dynamin superfamily protein which could play a role in bacteriophage resistance. In the resistant variants proteins associated with resistance mechanisms occurring in biofilm, e.g. polyphosphate kinase, flagella basal body rod protein were detected. These results indicate proteins important in the development of P. mirabilis antibiofilm therapies.Agnieszka MaszewskaMagdalena MorylJunli WuBin LiuLu FengAntoni RozalskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Agnieszka Maszewska Magdalena Moryl Junli Wu Bin Liu Lu Feng Antoni Rozalski Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm
description	Abstract Modification of outer membrane proteins (OMPs) is the first line of Gram-negative bacteria defence against antimicrobials. Here we point to Proteus mirabilis OMPs and their role in antibiotic and phage resistance. Protein profiles of amikacin (AMKrsv), phage (Brsv) and amikacin/phage (AMK/Brsv) resistant variants of P. mirabilis were compared to that obtained for a wild strain. In resistant variants there were identified 14, 1, 5 overexpressed and 13, 5, 1 downregulated proteins for AMKrsv, Brsv and AMK/Brsv, respectively. Application of phages with amikacin led to reducing the number of up- and downregulated proteins compared to single antibiotic treatment. Proteins isolated in AMKrsv are involved in protein biosynthesis, transcription and signal transduction, which correspond to well-known mechanisms of bacteria resistance to aminoglycosides. In isolated OMPs several cytoplasmic proteins, important in antibiotic resistance, were identified, probably as a result of environmental stress, e.g. elongation factor Tu, asparaginyl-tRNA and aspartyl-tRNA synthetases. In Brsv there were identified: NusA and dynamin superfamily protein which could play a role in bacteriophage resistance. In the resistant variants proteins associated with resistance mechanisms occurring in biofilm, e.g. polyphosphate kinase, flagella basal body rod protein were detected. These results indicate proteins important in the development of P. mirabilis antibiofilm therapies.
format	article
author	Agnieszka Maszewska Magdalena Moryl Junli Wu Bin Liu Lu Feng Antoni Rozalski
author_facet	Agnieszka Maszewska Magdalena Moryl Junli Wu Bin Liu Lu Feng Antoni Rozalski
author_sort	Agnieszka Maszewska
title	Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm
title_short	Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm
title_full	Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm
title_fullStr	Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm
title_full_unstemmed	Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm
title_sort	amikacin and bacteriophage treatment modulates outer membrane proteins composition in proteus mirabilis biofilm
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/531501436d654d0096ac7172dcb57f17
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Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm

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