Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement

Xiaoyue Zhou,1,2,* Shin-Hye Park,1,* Hongli Mao,3 Takashi Isoshima,1 Yi Wang,2 Yoshihiro Ito1,3 1Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan; 2Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People’s Republic...

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Autores principales:	Zhou X, Park SH, Mao H, Isoshima T, Wang Y, Ito Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/53181bf4c0cd4756bca20de1565843ac
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spelling	oai:doaj.org-article:53181bf4c0cd4756bca20de1565843ac2021-12-02T05:15:16ZNanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement1178-2013https://doaj.org/article/53181bf4c0cd4756bca20de1565843ac2015-09-01T00:00:00Zhttp://www.dovepress.com/nanolayer-formation-on-titanium-by-phosphonated-gelatin-for-cell-adhes-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xiaoyue Zhou,1,2,* Shin-Hye Park,1,* Hongli Mao,3 Takashi Isoshima,1 Yi Wang,2 Yoshihiro Ito1,3 1Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan; 2Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People’s Republic of China; 3Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, Wako, Saitama, Japan *These authors contributed equally to this work Abstract: Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by 31P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface. Keywords: phosphonated gelatin, surface modification, titanium, cell adhesionZhou XPark SHMao HIsoshima TWang YIto YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 5597-5607 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhou X Park SH Mao H Isoshima T Wang Y Ito Y Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement
description	Xiaoyue Zhou,1,2,* Shin-Hye Park,1,* Hongli Mao,3 Takashi Isoshima,1 Yi Wang,2 Yoshihiro Ito1,3 1Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan; 2Department of Regenerative Medicine, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, People’s Republic of China; 3Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, Wako, Saitama, Japan *These authors contributed equally to this work Abstract: Phosphonated gelatin was prepared for surface modification of titanium to stimulate cell functions. The modified gelatin was synthesized by coupling with 3-aminopropylphosphonic acid using water-soluble carbodiimide and characterized by 31P nuclear magnetic resonance and gel permeation chromatography. Circular dichroism revealed no differences in the conformations of unmodified and phosphonated gelatin. However, the gelation temperature was changed by the modification. Even a high concentration of modified gelatin did not form a gel at room temperature. Time-of-flight secondary ion mass spectrometry showed direct bonding between the phosphonated gelatin and the titanium surface after binding. The binding behavior of phosphonated gelatin on the titanium surface was quantitatively analyzed by a quartz crystal microbalance. Ellipsometry showed the formation of a several nanometer layer of gelatin on the surface. Contact angle measurement indicated that the modified titanium surface was hydrophobic. Enhancement of the attachment and spreading of MC-3T3L1 osteoblastic cells was observed on the phosphonated gelatin-modified titanium. These effects on cell adhesion also led to growth enhancement. Phosphonation of gelatin was effective for preparation of a cell-stimulating titanium surface. Keywords: phosphonated gelatin, surface modification, titanium, cell adhesion
format	article
author	Zhou X Park SH Mao H Isoshima T Wang Y Ito Y
author_facet	Zhou X Park SH Mao H Isoshima T Wang Y Ito Y
author_sort	Zhou X
title	Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement
title_short	Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement
title_full	Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement
title_fullStr	Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement
title_full_unstemmed	Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement
title_sort	nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/53181bf4c0cd4756bca20de1565843ac
work_keys_str_mv	AT zhoux nanolayerformationontitaniumbyphosphonatedgelatinforcelladhesionandgrowthenhancement AT parksh nanolayerformationontitaniumbyphosphonatedgelatinforcelladhesionandgrowthenhancement AT maoh nanolayerformationontitaniumbyphosphonatedgelatinforcelladhesionandgrowthenhancement AT isoshimat nanolayerformationontitaniumbyphosphonatedgelatinforcelladhesionandgrowthenhancement AT wangy nanolayerformationontitaniumbyphosphonatedgelatinforcelladhesionandgrowthenhancement AT itoy nanolayerformationontitaniumbyphosphonatedgelatinforcelladhesionandgrowthenhancement
_version_	1718400467978944512

Nanolayer formation on titanium by phosphonated gelatin for cell adhesion and growth enhancement

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