Identification of C21 Steroidal Glycosides from Gymnema sylvestre (Retz.) and Evaluation of Their Glucose Uptake Activities

Gymnema sylvestre (Retz.) Schult is a multi-purpose traditional medicine that has long been used for the treatment of various diseases. To discover the potential bioactive composition of G. sylvestre, a chemical investigation was thus performed. In this research...

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Autores principales:	Meiyu Liu, Tongxi Zhou, Jinyan Zhang, Guangfeng Liao, Rumei Lu, Xinzhou Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Gymnema sylvestre</i> C<sub>21</sub> steroidal glycosides glucose uptake GLUT-4 Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/5319c35b362e4caabdcaa0b659300e51
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spelling	oai:doaj.org-article:5319c35b362e4caabdcaa0b659300e512021-11-11T18:32:00ZIdentification of C<sub>21</sub> Steroidal Glycosides from <i>Gymnema sylvestre</i> (Retz.) and Evaluation of Their Glucose Uptake Activities10.3390/molecules262165491420-3049https://doaj.org/article/5319c35b362e4caabdcaa0b659300e512021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6549https://doaj.org/toc/1420-3049<i>Gymnema sylvestre</i> (Retz.) Schult is a multi-purpose traditional medicine that has long been used for the treatment of various diseases. To discover the potential bioactive composition of <i>G. sylvestre</i>, a chemical investigation was thus performed. In this research, four new C<sub>21</sub> steroidal glycosides sylvepregosides A-D (<b>1</b>–<b>4</b>) were isolated along with four known compounds, gymnepregoside H (<b>5</b>), deacetylkidjoladinin (<b>6</b>), gymnepregoside G (<b>7</b>) and gymnepregoside I (<b>8</b>), from the ethyl acetate fraction of <i>G. sylvestre</i>. The structures of the new compounds were established by extensive 1D and 2D nuclear magnetic resonance (NMR) spectra with mass spectroscopy data. Compounds <b>1</b>–<b>6</b> promoted glucose uptake by the range of 1.10- to 2.37-fold, respectively. Compound <b>1</b> showed the most potent glucose uptake, with 1.37-fold enhancement. Further study showed that compounds <b>1</b> and <b>5</b> could promote GLUT-4 fusion with the plasma membrane in L6 cells. The result attained in this study indicated that the separation and characterization of these compounds play an important role in the research and development of new anti-diabetic drugs and pharmaceutical industry.Meiyu LiuTongxi ZhouJinyan ZhangGuangfeng LiaoRumei LuXinzhou YangMDPI AGarticle<i>Gymnema sylvestre</i>C<sub>21</sub> steroidal glycosidesglucose uptakeGLUT-4Organic chemistryQD241-441ENMolecules, Vol 26, Iss 6549, p 6549 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Gymnema sylvestre</i> C<sub>21</sub> steroidal glycosides glucose uptake GLUT-4 Organic chemistry QD241-441
spellingShingle	<i>Gymnema sylvestre</i> C<sub>21</sub> steroidal glycosides glucose uptake GLUT-4 Organic chemistry QD241-441 Meiyu Liu Tongxi Zhou Jinyan Zhang Guangfeng Liao Rumei Lu Xinzhou Yang Identification of C<sub>21</sub> Steroidal Glycosides from <i>Gymnema sylvestre</i> (Retz.) and Evaluation of Their Glucose Uptake Activities
description	<i>Gymnema sylvestre</i> (Retz.) Schult is a multi-purpose traditional medicine that has long been used for the treatment of various diseases. To discover the potential bioactive composition of <i>G. sylvestre</i>, a chemical investigation was thus performed. In this research, four new C<sub>21</sub> steroidal glycosides sylvepregosides A-D (<b>1</b>–<b>4</b>) were isolated along with four known compounds, gymnepregoside H (<b>5</b>), deacetylkidjoladinin (<b>6</b>), gymnepregoside G (<b>7</b>) and gymnepregoside I (<b>8</b>), from the ethyl acetate fraction of <i>G. sylvestre</i>. The structures of the new compounds were established by extensive 1D and 2D nuclear magnetic resonance (NMR) spectra with mass spectroscopy data. Compounds <b>1</b>–<b>6</b> promoted glucose uptake by the range of 1.10- to 2.37-fold, respectively. Compound <b>1</b> showed the most potent glucose uptake, with 1.37-fold enhancement. Further study showed that compounds <b>1</b> and <b>5</b> could promote GLUT-4 fusion with the plasma membrane in L6 cells. The result attained in this study indicated that the separation and characterization of these compounds play an important role in the research and development of new anti-diabetic drugs and pharmaceutical industry.
format	article
author	Meiyu Liu Tongxi Zhou Jinyan Zhang Guangfeng Liao Rumei Lu Xinzhou Yang
author_facet	Meiyu Liu Tongxi Zhou Jinyan Zhang Guangfeng Liao Rumei Lu Xinzhou Yang
author_sort	Meiyu Liu
title	Identification of C<sub>21</sub> Steroidal Glycosides from <i>Gymnema sylvestre</i> (Retz.) and Evaluation of Their Glucose Uptake Activities
title_short	Identification of C<sub>21</sub> Steroidal Glycosides from <i>Gymnema sylvestre</i> (Retz.) and Evaluation of Their Glucose Uptake Activities
title_full	Identification of C<sub>21</sub> Steroidal Glycosides from <i>Gymnema sylvestre</i> (Retz.) and Evaluation of Their Glucose Uptake Activities
title_fullStr	Identification of C<sub>21</sub> Steroidal Glycosides from <i>Gymnema sylvestre</i> (Retz.) and Evaluation of Their Glucose Uptake Activities
title_full_unstemmed	Identification of C<sub>21</sub> Steroidal Glycosides from <i>Gymnema sylvestre</i> (Retz.) and Evaluation of Their Glucose Uptake Activities
title_sort	identification of c<sub>21</sub> steroidal glycosides from <i>gymnema sylvestre</i> (retz.) and evaluation of their glucose uptake activities
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5319c35b362e4caabdcaa0b659300e51
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Identification of C<sub>21</sub> Steroidal Glycosides from <i>Gymnema sylvestre</i> (Retz.) and Evaluation of Their Glucose Uptake Activities

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