Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria

Ghofran Hasan Alshareef,1 Afrah E Mohammed,1 Mohammed Abumaree2,3 ,† Yasser S Basmaeil2 1Biology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, 84428, Saudi Arabia; 2Stem Cell & Regenerative Medicine Department, King Abdullah International Medical Research C...

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Autores principales:	Alshareef GH, Mohammed AE, Abumaree M, Basmaeil YS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	placenta endothelial cells proliferation adhesion migration real-time pcr Cytology QH573-671
Acceso en línea:	https://doaj.org/article/531fb608845d4882864bfc8a7bc2d68c
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spelling	oai:doaj.org-article:531fb608845d4882864bfc8a7bc2d68c2021-12-02T19:31:58ZPhenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria1178-6957https://doaj.org/article/531fb608845d4882864bfc8a7bc2d68c2021-11-01T00:00:00Zhttps://www.dovepress.com/phenotypic-and-functional-responses-of-human-decidua-basalis-mesenchym-peer-reviewed-fulltext-article-SCCAAhttps://doaj.org/toc/1178-6957Ghofran Hasan Alshareef,1 Afrah E Mohammed,1 Mohammed Abumaree2,3 ,† Yasser S Basmaeil2 1Biology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, 84428, Saudi Arabia; 2Stem Cell & Regenerative Medicine Department, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia; 3College of Science and Health Professions, King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Riyadh, 11481, Saudi Arabia†Prof. Mohammed Abumaree passed away on 26.07.2019.Correspondence: Afrah E Mohammed; Yasser S BasmaeilStem Cell & Regenerative Medicine Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh, Saudi ArabiaTel +966 11 8011111 Ext: 94573Email AFAMohammed@pnu.edu.sa; basmaeily@ngha.med.saIntroduction: Human decidua basalis mesenchymal stem cells (DBMSCs) are potential therapeutics for the medication to cure inflammatory diseases, like atherosclerosis. The current study investigates the capacity of DBMSCs to stay alive and function in a harmful inflammatory environment induced by high levels of lipopolysaccharide (LPS).Methods: DBMSCs were exposed to different levels of LPS, and their viability and functional responses (proliferation, adhesion, and migration) were examined. Furthermore, DBMSCs’ expression of 84 genes associated with their functional activities in the presence of LPS was investigated.Results: Results indicated that LPS had no significant effect on DBMSCs’ adhesion, migration, and proliferation (24 h and 72 h) (p > 0.05). However, DBMSCs’ proliferation was significantly reduced at 10 μg/mL of LPS at 48 h (p < 0.05). In addition, inflammatory cytokines and receptors related to adhesion, proliferation, migration, and differentiation were significantly overexpressed when DBMSCs were treated with 10 μg/mL of LPS (p < 0.05).Conclusion: These results indicated that DBMSCs maintained their functional activities (proliferation, adhesion, and migration) in the presence of LPS as there was no variation between the treated DBMSCs and the control group. This study will lay the foundation for future preclinical and clinical studies to confirm the appropriateness of DBMSCs as a potential medication to cure inflammatory diseases, like atherosclerosis.Keywords: placenta, endothelial cells, proliferation, adhesion, migration, real-time PCRAlshareef GHMohammed AEAbumaree MBasmaeil YSDove Medical Pressarticleplacentaendothelial cellsproliferationadhesionmigrationreal-time pcrCytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol Volume 14, Pp 51-69 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	placenta endothelial cells proliferation adhesion migration real-time pcr Cytology QH573-671
spellingShingle	placenta endothelial cells proliferation adhesion migration real-time pcr Cytology QH573-671 Alshareef GH Mohammed AE Abumaree M Basmaeil YS Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria
description	Ghofran Hasan Alshareef,1 Afrah E Mohammed,1 Mohammed Abumaree2,3 ,† Yasser S Basmaeil2 1Biology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, 84428, Saudi Arabia; 2Stem Cell & Regenerative Medicine Department, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia; 3College of Science and Health Professions, King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Riyadh, 11481, Saudi Arabia†Prof. Mohammed Abumaree passed away on 26.07.2019.Correspondence: Afrah E Mohammed; Yasser S BasmaeilStem Cell & Regenerative Medicine Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh, Saudi ArabiaTel +966 11 8011111 Ext: 94573Email AFAMohammed@pnu.edu.sa; basmaeily@ngha.med.saIntroduction: Human decidua basalis mesenchymal stem cells (DBMSCs) are potential therapeutics for the medication to cure inflammatory diseases, like atherosclerosis. The current study investigates the capacity of DBMSCs to stay alive and function in a harmful inflammatory environment induced by high levels of lipopolysaccharide (LPS).Methods: DBMSCs were exposed to different levels of LPS, and their viability and functional responses (proliferation, adhesion, and migration) were examined. Furthermore, DBMSCs’ expression of 84 genes associated with their functional activities in the presence of LPS was investigated.Results: Results indicated that LPS had no significant effect on DBMSCs’ adhesion, migration, and proliferation (24 h and 72 h) (p > 0.05). However, DBMSCs’ proliferation was significantly reduced at 10 μg/mL of LPS at 48 h (p < 0.05). In addition, inflammatory cytokines and receptors related to adhesion, proliferation, migration, and differentiation were significantly overexpressed when DBMSCs were treated with 10 μg/mL of LPS (p < 0.05).Conclusion: These results indicated that DBMSCs maintained their functional activities (proliferation, adhesion, and migration) in the presence of LPS as there was no variation between the treated DBMSCs and the control group. This study will lay the foundation for future preclinical and clinical studies to confirm the appropriateness of DBMSCs as a potential medication to cure inflammatory diseases, like atherosclerosis.Keywords: placenta, endothelial cells, proliferation, adhesion, migration, real-time PCR
format	article
author	Alshareef GH Mohammed AE Abumaree M Basmaeil YS
author_facet	Alshareef GH Mohammed AE Abumaree M Basmaeil YS
author_sort	Alshareef GH
title	Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria
title_short	Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria
title_full	Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria
title_fullStr	Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria
title_full_unstemmed	Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria
title_sort	phenotypic and functional responses of human decidua basalis mesenchymal stem/stromal cells to lipopolysaccharide of gram-negative bacteria
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/531fb608845d4882864bfc8a7bc2d68c
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Phenotypic and Functional Responses of Human Decidua Basalis Mesenchymal Stem/Stromal Cells to Lipopolysaccharide of Gram-Negative Bacteria

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