Dietary quenching enzyme AiiO-AIO6 promotes fish growth through intestinal microbiota: Zebrafish as a model

The growth performance of cultured aquatic animals is directly related to the economic benefit of the aquaculture industry. Quenching enzyme AiiO-AIO6 could degrade quorum sensing signal molecules N-acylhomoserine lactone (AHL), in this work, the effects of this AiiO-AIO6 on the growth of zebrafish...

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Autores principales:	Yuan-Yuan Yao, Rui Xia, Ya-Lin Yang, Chen-Chen Gao, Feng-Li Zhang, Qiang Hao, Chao Ran, Zhen Zhang, Zhi-Gang Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Quenching enzyme Intestinal microbiota Growth promotion Germ-free zebrafish Aquaculture. Fisheries. Angling SH1-691
Acceso en línea:	https://doaj.org/article/53272e8e0f364f52b22767f1b12f0a1f
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spelling	oai:doaj.org-article:53272e8e0f364f52b22767f1b12f0a1f2021-11-20T05:07:20ZDietary quenching enzyme AiiO-AIO6 promotes fish growth through intestinal microbiota: Zebrafish as a model2352-513410.1016/j.aqrep.2021.100947https://doaj.org/article/53272e8e0f364f52b22767f1b12f0a1f2022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352513421003641https://doaj.org/toc/2352-5134The growth performance of cultured aquatic animals is directly related to the economic benefit of the aquaculture industry. Quenching enzyme AiiO-AIO6 could degrade quorum sensing signal molecules N-acylhomoserine lactone (AHL), in this work, the effects of this AiiO-AIO6 on the growth of zebrafish was studied, the control group and AiiO-AIO6 additive group were set up with 120 fish in each group, and the feeding experiment was carried out for three weeks. The following results were obtained by observation of intestinal structure and morphology, quantitative PCR, gut microbes sequencing analysis and transfer, etc. The results showed that the final weight, weight gain rate and specific growth rate were significantly increased and feed conversion ratio and feeding rate of AiiO-AIO6 treated group were significantly decreased. Intestinal digestive enzyme assay showed that there was no significant difference in amylase, lipase and protease activities between the AiiO-AIO6 and control group. Hematoxylin and eosin (H&E) staining of intestinal morphology showed that the height of villus, the number of goblet cells and the height of columnar epithelial cells were significantly increased in the AiiO-AIO6 group. In addition, the relative expression levels of intestinal nutrient transporters Pept1a, GLUT2 and FATP1-a were also significantly increased. The analysis of intestinal microbiota structure and short-chain fatty acids showed that the addition of AiiO-AIO6 could change intestinal microbiota structure and increase the contents of acetic acid and isovalerate. The expression of growth-related genes in intestine, liver and muscle of two groups showed that growth hormone GH, GH receptor GHra, insulin-like growth factor IGF-I and myogenic factor Myf5 of AiiO-AIO6 group were significantly up-regulated. Results in the germ-free zebrafish model showed that the expression of growth-related genes was not directly regulated by AiiO-AIO6, but was mediated by the action of active intestinal microbiota. Overall, our data reflected that AiiO-AIO6 can promote the growth performance of zebrafish, and AiiO-AIO6 is a potential functional additive to make aquaculture efficient and high-yielding.Yuan-Yuan YaoRui XiaYa-Lin YangChen-Chen GaoFeng-Li ZhangQiang HaoChao RanZhen ZhangZhi-Gang ZhouElsevierarticleQuenching enzymeIntestinal microbiotaGrowth promotionGerm-free zebrafishAquaculture. Fisheries. AnglingSH1-691ENAquaculture Reports, Vol 22, Iss , Pp 100947- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Quenching enzyme Intestinal microbiota Growth promotion Germ-free zebrafish Aquaculture. Fisheries. Angling SH1-691
spellingShingle	Quenching enzyme Intestinal microbiota Growth promotion Germ-free zebrafish Aquaculture. Fisheries. Angling SH1-691 Yuan-Yuan Yao Rui Xia Ya-Lin Yang Chen-Chen Gao Feng-Li Zhang Qiang Hao Chao Ran Zhen Zhang Zhi-Gang Zhou Dietary quenching enzyme AiiO-AIO6 promotes fish growth through intestinal microbiota: Zebrafish as a model
description	The growth performance of cultured aquatic animals is directly related to the economic benefit of the aquaculture industry. Quenching enzyme AiiO-AIO6 could degrade quorum sensing signal molecules N-acylhomoserine lactone (AHL), in this work, the effects of this AiiO-AIO6 on the growth of zebrafish was studied, the control group and AiiO-AIO6 additive group were set up with 120 fish in each group, and the feeding experiment was carried out for three weeks. The following results were obtained by observation of intestinal structure and morphology, quantitative PCR, gut microbes sequencing analysis and transfer, etc. The results showed that the final weight, weight gain rate and specific growth rate were significantly increased and feed conversion ratio and feeding rate of AiiO-AIO6 treated group were significantly decreased. Intestinal digestive enzyme assay showed that there was no significant difference in amylase, lipase and protease activities between the AiiO-AIO6 and control group. Hematoxylin and eosin (H&E) staining of intestinal morphology showed that the height of villus, the number of goblet cells and the height of columnar epithelial cells were significantly increased in the AiiO-AIO6 group. In addition, the relative expression levels of intestinal nutrient transporters Pept1a, GLUT2 and FATP1-a were also significantly increased. The analysis of intestinal microbiota structure and short-chain fatty acids showed that the addition of AiiO-AIO6 could change intestinal microbiota structure and increase the contents of acetic acid and isovalerate. The expression of growth-related genes in intestine, liver and muscle of two groups showed that growth hormone GH, GH receptor GHra, insulin-like growth factor IGF-I and myogenic factor Myf5 of AiiO-AIO6 group were significantly up-regulated. Results in the germ-free zebrafish model showed that the expression of growth-related genes was not directly regulated by AiiO-AIO6, but was mediated by the action of active intestinal microbiota. Overall, our data reflected that AiiO-AIO6 can promote the growth performance of zebrafish, and AiiO-AIO6 is a potential functional additive to make aquaculture efficient and high-yielding.
format	article
author	Yuan-Yuan Yao Rui Xia Ya-Lin Yang Chen-Chen Gao Feng-Li Zhang Qiang Hao Chao Ran Zhen Zhang Zhi-Gang Zhou
author_facet	Yuan-Yuan Yao Rui Xia Ya-Lin Yang Chen-Chen Gao Feng-Li Zhang Qiang Hao Chao Ran Zhen Zhang Zhi-Gang Zhou
author_sort	Yuan-Yuan Yao
title	Dietary quenching enzyme AiiO-AIO6 promotes fish growth through intestinal microbiota: Zebrafish as a model
title_short	Dietary quenching enzyme AiiO-AIO6 promotes fish growth through intestinal microbiota: Zebrafish as a model
title_full	Dietary quenching enzyme AiiO-AIO6 promotes fish growth through intestinal microbiota: Zebrafish as a model
title_fullStr	Dietary quenching enzyme AiiO-AIO6 promotes fish growth through intestinal microbiota: Zebrafish as a model
title_full_unstemmed	Dietary quenching enzyme AiiO-AIO6 promotes fish growth through intestinal microbiota: Zebrafish as a model
title_sort	dietary quenching enzyme aiio-aio6 promotes fish growth through intestinal microbiota: zebrafish as a model
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/53272e8e0f364f52b22767f1b12f0a1f
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Dietary quenching enzyme AiiO-AIO6 promotes fish growth through intestinal microbiota: Zebrafish as a model

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