Structural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of Rubber

Structural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of Rubber

Abstract Latex clearing proteins (Lcps) are rubber oxygenases that catalyse the extracellular cleavage of poly (cis-1,4-isoprene) by Gram-positive rubber degrading bacteria. Lcp of Streptomyces sp. K30 (LcpK30) is a b-type cytochrome and acts as an endo-type dioxygenase producing C20 and higher olig...

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Autores principales: Lorena Ilcu, Wolf Röther, Jakob Birke, Anton Brausemann, Oliver Einsle, Dieter Jendrossek
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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spelling oai:doaj.org-article:532ad933220c47fabfed16e8602af9ad2021-12-02T11:40:58ZStructural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of Rubber10.1038/s41598-017-05268-22045-2322https://doaj.org/article/532ad933220c47fabfed16e8602af9ad2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05268-2https://doaj.org/toc/2045-2322Abstract Latex clearing proteins (Lcps) are rubber oxygenases that catalyse the extracellular cleavage of poly (cis-1,4-isoprene) by Gram-positive rubber degrading bacteria. Lcp of Streptomyces sp. K30 (LcpK30) is a b-type cytochrome and acts as an endo-type dioxygenase producing C20 and higher oligo-isoprenoids that differ in the number of isoprene units but have the same terminal functions, CHO-CH2– and –CH2-COCH3. Our analysis of the LcpK30 structure revealed a 3/3 globin fold with additional domains at the N- and C-termini and similarities to globin-coupled sensor proteins. The haem group of LcpK30 is ligated to the polypeptide by a proximal histidine (His198) and by a lysine residue (Lys167) as the distal axial ligand. The comparison of LcpK30 structures in a closed and in an open state as well as spectroscopic and biochemical analysis of wild type and LcpK30 muteins provided insights into the action of the enzyme during catalysis.Lorena IlcuWolf RötherJakob BirkeAnton BrausemannOliver EinsleDieter JendrossekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Lorena Ilcu
Wolf Röther
Jakob Birke
Anton Brausemann
Oliver Einsle
Dieter Jendrossek
Structural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of Rubber
description Abstract Latex clearing proteins (Lcps) are rubber oxygenases that catalyse the extracellular cleavage of poly (cis-1,4-isoprene) by Gram-positive rubber degrading bacteria. Lcp of Streptomyces sp. K30 (LcpK30) is a b-type cytochrome and acts as an endo-type dioxygenase producing C20 and higher oligo-isoprenoids that differ in the number of isoprene units but have the same terminal functions, CHO-CH2– and –CH2-COCH3. Our analysis of the LcpK30 structure revealed a 3/3 globin fold with additional domains at the N- and C-termini and similarities to globin-coupled sensor proteins. The haem group of LcpK30 is ligated to the polypeptide by a proximal histidine (His198) and by a lysine residue (Lys167) as the distal axial ligand. The comparison of LcpK30 structures in a closed and in an open state as well as spectroscopic and biochemical analysis of wild type and LcpK30 muteins provided insights into the action of the enzyme during catalysis.
format article
author Lorena Ilcu
Wolf Röther
Jakob Birke
Anton Brausemann
Oliver Einsle
Dieter Jendrossek
author_facet Lorena Ilcu
Wolf Röther
Jakob Birke
Anton Brausemann
Oliver Einsle
Dieter Jendrossek
author_sort Lorena Ilcu
title Structural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of Rubber
title_short Structural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of Rubber
title_full Structural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of Rubber
title_fullStr Structural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of Rubber
title_full_unstemmed Structural and Functional Analysis of Latex Clearing Protein (Lcp) Provides Insight into the Enzymatic Cleavage of Rubber
title_sort structural and functional analysis of latex clearing protein (lcp) provides insight into the enzymatic cleavage of rubber
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/532ad933220c47fabfed16e8602af9ad
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