Disparities in the gut metabolome of post-operative Hirschsprung's disease patients

Abstract Hirschsprung's disease (HD) is a congenital structural abnormality of the colon seen in approximately 1 to 5000 live births. Despite surgical correction shortly after presentation, up to 60% of patients will express long-term gastrointestinal complaints, including potentially life-thre...

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Autores principales:	Vera Plekhova, Ellen De Paepe, Katrien Van Renterghem, Myriam Van Winckel, Lieselot Y. Hemeryck, Lynn Vanhaecke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/532c0cc81a17457fbe5a8aebda259ae4
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spelling	oai:doaj.org-article:532c0cc81a17457fbe5a8aebda259ae42021-12-02T19:06:40ZDisparities in the gut metabolome of post-operative Hirschsprung's disease patients10.1038/s41598-021-95589-02045-2322https://doaj.org/article/532c0cc81a17457fbe5a8aebda259ae42021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95589-0https://doaj.org/toc/2045-2322Abstract Hirschsprung's disease (HD) is a congenital structural abnormality of the colon seen in approximately 1 to 5000 live births. Despite surgical correction shortly after presentation, up to 60% of patients will express long-term gastrointestinal complaints, including potentially life-threatening Hirschsprung-associated enterocolitis (HAEC). In this study fecal samples from postoperative HD patients (n = 38) and their healthy siblings (n = 21) were analysed using high-resolution liquid chromatography—mass spectrometry aiming to further unravel the nature of the chronic gastrointestinal disturbances. Furthermore, within the patient group, we compared the faecal metabolome between patients with and without a history of HAEC as well as those diagnosed with short or long aganglionic segment. Targeted analysis identified several individual metabolites characteristic for all HD patients as well as those with a history of HAEC and long segment HD. Moreover, multivariate models based on untargeted data established statistically significant (p < 0.05) differences in comprehensive faecal metabolome in the patients’ cohort as a whole and in patients with a history of HAEC. Pathway analysis revealed the most impact on amino sugar, lysine, sialic acid, hyaluronan and heparan sulphate metabolism in HD, as well as impaired tyrosine metabolism in HAEC group. Those changes imply disruption of intestinal mucosal barrier due to glycosaminoglycan breakdown and dysbiosis as major metabolic changes in patients’ group and should be further explored for potential diagnostic or treatment targets.Vera PlekhovaEllen De PaepeKatrien Van RenterghemMyriam Van WinckelLieselot Y. HemeryckLynn VanhaeckeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vera Plekhova Ellen De Paepe Katrien Van Renterghem Myriam Van Winckel Lieselot Y. Hemeryck Lynn Vanhaecke Disparities in the gut metabolome of post-operative Hirschsprung's disease patients
description	Abstract Hirschsprung's disease (HD) is a congenital structural abnormality of the colon seen in approximately 1 to 5000 live births. Despite surgical correction shortly after presentation, up to 60% of patients will express long-term gastrointestinal complaints, including potentially life-threatening Hirschsprung-associated enterocolitis (HAEC). In this study fecal samples from postoperative HD patients (n = 38) and their healthy siblings (n = 21) were analysed using high-resolution liquid chromatography—mass spectrometry aiming to further unravel the nature of the chronic gastrointestinal disturbances. Furthermore, within the patient group, we compared the faecal metabolome between patients with and without a history of HAEC as well as those diagnosed with short or long aganglionic segment. Targeted analysis identified several individual metabolites characteristic for all HD patients as well as those with a history of HAEC and long segment HD. Moreover, multivariate models based on untargeted data established statistically significant (p < 0.05) differences in comprehensive faecal metabolome in the patients’ cohort as a whole and in patients with a history of HAEC. Pathway analysis revealed the most impact on amino sugar, lysine, sialic acid, hyaluronan and heparan sulphate metabolism in HD, as well as impaired tyrosine metabolism in HAEC group. Those changes imply disruption of intestinal mucosal barrier due to glycosaminoglycan breakdown and dysbiosis as major metabolic changes in patients’ group and should be further explored for potential diagnostic or treatment targets.
format	article
author	Vera Plekhova Ellen De Paepe Katrien Van Renterghem Myriam Van Winckel Lieselot Y. Hemeryck Lynn Vanhaecke
author_facet	Vera Plekhova Ellen De Paepe Katrien Van Renterghem Myriam Van Winckel Lieselot Y. Hemeryck Lynn Vanhaecke
author_sort	Vera Plekhova
title	Disparities in the gut metabolome of post-operative Hirschsprung's disease patients
title_short	Disparities in the gut metabolome of post-operative Hirschsprung's disease patients
title_full	Disparities in the gut metabolome of post-operative Hirschsprung's disease patients
title_fullStr	Disparities in the gut metabolome of post-operative Hirschsprung's disease patients
title_full_unstemmed	Disparities in the gut metabolome of post-operative Hirschsprung's disease patients
title_sort	disparities in the gut metabolome of post-operative hirschsprung's disease patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/532c0cc81a17457fbe5a8aebda259ae4
work_keys_str_mv	AT veraplekhova disparitiesinthegutmetabolomeofpostoperativehirschsprungsdiseasepatients AT ellendepaepe disparitiesinthegutmetabolomeofpostoperativehirschsprungsdiseasepatients AT katrienvanrenterghem disparitiesinthegutmetabolomeofpostoperativehirschsprungsdiseasepatients AT myriamvanwinckel disparitiesinthegutmetabolomeofpostoperativehirschsprungsdiseasepatients AT lieselotyhemeryck disparitiesinthegutmetabolomeofpostoperativehirschsprungsdiseasepatients AT lynnvanhaecke disparitiesinthegutmetabolomeofpostoperativehirschsprungsdiseasepatients
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Disparities in the gut metabolome of post-operative Hirschsprung's disease patients

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