Down-Regulation of Phosphoenolpyruvate Carboxylase Kinase in Grapevine Cell Cultures and Leaves Is Linked to Enhanced Resveratrol Biosynthesis

In grapevine, trans-Resveratrol (tR) is produced as a defence mechanism against stress or infection. tR is also considered to be important for human health, which increases its interest to the scientific community. Transcriptomic analysis in grapevine cell cultures treated with the defence response...

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Autores principales:	Elías Hurtado-Gaitán, Susana Sellés-Marchart, James Hartwell, Maria José Martínez-Esteso, Roque Bru-Martínez
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	PEP carboxylase kinase resveratrol biosynthesis regulation elicitation cell culture <i>Vitis vinifera</i> Gamay Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/532fe8d3dd0041bb8f7d2d741651f78f
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spelling	oai:doaj.org-article:532fe8d3dd0041bb8f7d2d741651f78f2021-11-25T16:53:17ZDown-Regulation of Phosphoenolpyruvate Carboxylase Kinase in Grapevine Cell Cultures and Leaves Is Linked to Enhanced Resveratrol Biosynthesis10.3390/biom111116412218-273Xhttps://doaj.org/article/532fe8d3dd0041bb8f7d2d741651f78f2021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1641https://doaj.org/toc/2218-273XIn grapevine, trans-Resveratrol (tR) is produced as a defence mechanism against stress or infection. tR is also considered to be important for human health, which increases its interest to the scientific community. Transcriptomic analysis in grapevine cell cultures treated with the defence response elicitor methyl-β-cyclodextrin (CD) revealed that both copies of PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE (<i>PPCK</i>) were down-regulated significantly. A role for PPCK in the defence response pathway has not been proposed previously. We therefore analysed the control of <i>PPCK</i> transcript levels in grapevine cell cultures and leaves elicited with CD. Moreover, phosphoenolpyruvate carboxylase (<i>PPC</i>), stilbene synthase (<i>STS</i>), and the transcription factors <i>MYB14</i> and <i>WRKY24,</i> which are involved in the activation of <i>STS</i> transcription, were also analysed by RT-qPCR. The results revealed that under CD elicitation conditions <i>PPCK</i> down-regulation, increased stilbene production and loss of PPC activity occurs in both tissues. Moreover, <i>STS</i> transcripts were co-induced with <i>MYB14</i> and <i>WRKY24</i> in cell cultures and leaves. These genes have not previously been reported to respond to CD in grape leaves. Our findings thus support the hypothesis that PPCK is involved in diverting metabolism towards stilbene biosynthesis, both for in vitro cell culture and whole leaves. We thus provide new evidence for PEP being redirected between primary and secondary metabolism to support tR production and the stress response.Elías Hurtado-GaitánSusana Sellés-MarchartJames HartwellMaria José Martínez-EstesoRoque Bru-MartínezMDPI AGarticlePEP carboxylase kinaseresveratrol biosynthesis regulationelicitationcell culture<i>Vitis vinifera</i>GamayMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1641, p 1641 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PEP carboxylase kinase resveratrol biosynthesis regulation elicitation cell culture <i>Vitis vinifera</i> Gamay Microbiology QR1-502
spellingShingle	PEP carboxylase kinase resveratrol biosynthesis regulation elicitation cell culture <i>Vitis vinifera</i> Gamay Microbiology QR1-502 Elías Hurtado-Gaitán Susana Sellés-Marchart James Hartwell Maria José Martínez-Esteso Roque Bru-Martínez Down-Regulation of Phosphoenolpyruvate Carboxylase Kinase in Grapevine Cell Cultures and Leaves Is Linked to Enhanced Resveratrol Biosynthesis
description	In grapevine, trans-Resveratrol (tR) is produced as a defence mechanism against stress or infection. tR is also considered to be important for human health, which increases its interest to the scientific community. Transcriptomic analysis in grapevine cell cultures treated with the defence response elicitor methyl-β-cyclodextrin (CD) revealed that both copies of PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE (<i>PPCK</i>) were down-regulated significantly. A role for PPCK in the defence response pathway has not been proposed previously. We therefore analysed the control of <i>PPCK</i> transcript levels in grapevine cell cultures and leaves elicited with CD. Moreover, phosphoenolpyruvate carboxylase (<i>PPC</i>), stilbene synthase (<i>STS</i>), and the transcription factors <i>MYB14</i> and <i>WRKY24,</i> which are involved in the activation of <i>STS</i> transcription, were also analysed by RT-qPCR. The results revealed that under CD elicitation conditions <i>PPCK</i> down-regulation, increased stilbene production and loss of PPC activity occurs in both tissues. Moreover, <i>STS</i> transcripts were co-induced with <i>MYB14</i> and <i>WRKY24</i> in cell cultures and leaves. These genes have not previously been reported to respond to CD in grape leaves. Our findings thus support the hypothesis that PPCK is involved in diverting metabolism towards stilbene biosynthesis, both for in vitro cell culture and whole leaves. We thus provide new evidence for PEP being redirected between primary and secondary metabolism to support tR production and the stress response.
format	article
author	Elías Hurtado-Gaitán Susana Sellés-Marchart James Hartwell Maria José Martínez-Esteso Roque Bru-Martínez
author_facet	Elías Hurtado-Gaitán Susana Sellés-Marchart James Hartwell Maria José Martínez-Esteso Roque Bru-Martínez
author_sort	Elías Hurtado-Gaitán
title	Down-Regulation of Phosphoenolpyruvate Carboxylase Kinase in Grapevine Cell Cultures and Leaves Is Linked to Enhanced Resveratrol Biosynthesis
title_short	Down-Regulation of Phosphoenolpyruvate Carboxylase Kinase in Grapevine Cell Cultures and Leaves Is Linked to Enhanced Resveratrol Biosynthesis
title_full	Down-Regulation of Phosphoenolpyruvate Carboxylase Kinase in Grapevine Cell Cultures and Leaves Is Linked to Enhanced Resveratrol Biosynthesis
title_fullStr	Down-Regulation of Phosphoenolpyruvate Carboxylase Kinase in Grapevine Cell Cultures and Leaves Is Linked to Enhanced Resveratrol Biosynthesis
title_full_unstemmed	Down-Regulation of Phosphoenolpyruvate Carboxylase Kinase in Grapevine Cell Cultures and Leaves Is Linked to Enhanced Resveratrol Biosynthesis
title_sort	down-regulation of phosphoenolpyruvate carboxylase kinase in grapevine cell cultures and leaves is linked to enhanced resveratrol biosynthesis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/532fe8d3dd0041bb8f7d2d741651f78f
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Down-Regulation of Phosphoenolpyruvate Carboxylase Kinase in Grapevine Cell Cultures and Leaves Is Linked to Enhanced Resveratrol Biosynthesis

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