Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach

Abstract Enteroviruses (EVs) causing persisting infection are characterized by minimal replication and genetic changes. Typing of these agents may complement disease assessment and shed light on pathogenesis. Here we report an integrated approach for EV detection in human samples that is based on pr...

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Autores principales:	Angelo Genoni, Filippo Canducci, Agostino Rossi, Francesco Broccolo, Konstantin Chumakov, Giorgio Bono, Jorge Salerno-Uriarte, Alessandro Salvatoni, Alberto Pugliese, Antonio Toniolo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5331d48e6ec546f79f3bcc3a9521a0f1
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spelling	oai:doaj.org-article:5331d48e6ec546f79f3bcc3a9521a0f12021-12-02T12:30:53ZRevealing enterovirus infection in chronic human disorders: An integrated diagnostic approach10.1038/s41598-017-04993-y2045-2322https://doaj.org/article/5331d48e6ec546f79f3bcc3a9521a0f12017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04993-yhttps://doaj.org/toc/2045-2322Abstract Enteroviruses (EVs) causing persisting infection are characterized by minimal replication and genetic changes. Typing of these agents may complement disease assessment and shed light on pathogenesis. Here we report an integrated approach for EV detection in human samples that is based on pre-enrichment of virus in cell culture before search for the viral genome and viral antigens. Cases of post-polio syndrome, type 1 diabetes, and chronic cardiomyopathy were investigated. As tissue-based approaches require invasive procedures, information was mainly gleaned from virus in blood. Molecular assays targeting conserved genome regions of all EV types (5′UTR, 2 C, 3Dpol) were employed. As compared to direct assays of plasma or leukocytes, the EV detection rate was significantly enhanced by co-culture of leukocytes with cell lines prior to molecular and immunologic tests. Results of RT-PCR and sequencing were confirmed by staining cell cultures with a panel of EV-specific antibodies. Sequence and phylogenetic analysis showed that EVs of the C species (polioviruses) were associated with the post-polio syndrome, while members of the B species were found in type 1 diabetes and cardiomyopathy. The procedure may be used for investigating the possible association of different EVs with a variety of chronic neurologic, endocrine, and cardiac disorders.Angelo GenoniFilippo CanducciAgostino RossiFrancesco BroccoloKonstantin ChumakovGiorgio BonoJorge Salerno-UriarteAlessandro SalvatoniAlberto PuglieseAntonio TonioloNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Angelo Genoni Filippo Canducci Agostino Rossi Francesco Broccolo Konstantin Chumakov Giorgio Bono Jorge Salerno-Uriarte Alessandro Salvatoni Alberto Pugliese Antonio Toniolo Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach
description	Abstract Enteroviruses (EVs) causing persisting infection are characterized by minimal replication and genetic changes. Typing of these agents may complement disease assessment and shed light on pathogenesis. Here we report an integrated approach for EV detection in human samples that is based on pre-enrichment of virus in cell culture before search for the viral genome and viral antigens. Cases of post-polio syndrome, type 1 diabetes, and chronic cardiomyopathy were investigated. As tissue-based approaches require invasive procedures, information was mainly gleaned from virus in blood. Molecular assays targeting conserved genome regions of all EV types (5′UTR, 2 C, 3Dpol) were employed. As compared to direct assays of plasma or leukocytes, the EV detection rate was significantly enhanced by co-culture of leukocytes with cell lines prior to molecular and immunologic tests. Results of RT-PCR and sequencing were confirmed by staining cell cultures with a panel of EV-specific antibodies. Sequence and phylogenetic analysis showed that EVs of the C species (polioviruses) were associated with the post-polio syndrome, while members of the B species were found in type 1 diabetes and cardiomyopathy. The procedure may be used for investigating the possible association of different EVs with a variety of chronic neurologic, endocrine, and cardiac disorders.
format	article
author	Angelo Genoni Filippo Canducci Agostino Rossi Francesco Broccolo Konstantin Chumakov Giorgio Bono Jorge Salerno-Uriarte Alessandro Salvatoni Alberto Pugliese Antonio Toniolo
author_facet	Angelo Genoni Filippo Canducci Agostino Rossi Francesco Broccolo Konstantin Chumakov Giorgio Bono Jorge Salerno-Uriarte Alessandro Salvatoni Alberto Pugliese Antonio Toniolo
author_sort	Angelo Genoni
title	Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach
title_short	Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach
title_full	Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach
title_fullStr	Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach
title_full_unstemmed	Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach
title_sort	revealing enterovirus infection in chronic human disorders: an integrated diagnostic approach
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5331d48e6ec546f79f3bcc3a9521a0f1
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_version_	1718394278665781248

Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach

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