MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS STRAINS CIRCULATING IN THE URAL REGION, RUSSIA

Abstract. Overall 178 Mycobacterium tuberculosis isolates recovered in 2009–2011 from newly diagnosed epidemiologically unlinked to TB patients in the Ural region of Russia have been studied. The absolute concentration method was used for drug susceptibility testing. Mutations katG, inhA and rpoB as...

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Autores principales:	T. V. Umpeleva, M. A. Kravchenko, N. I. Eremeeva, A. A. Vyazovaya, O. V. Narvskaya
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2014
Materias:	mycobacterium tuberculosis genotyping vntr tb-biochip Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/5333b9715def421caa5a6b99f9521789
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spelling	oai:doaj.org-article:5333b9715def421caa5a6b99f95217892021-11-22T07:09:46ZMOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS STRAINS CIRCULATING IN THE URAL REGION, RUSSIA2220-76192313-739810.15789/2220-7619-2013-1-21-28https://doaj.org/article/5333b9715def421caa5a6b99f95217892014-07-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/119https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Abstract. Overall 178 Mycobacterium tuberculosis isolates recovered in 2009–2011 from newly diagnosed epidemiologically unlinked to TB patients in the Ural region of Russia have been studied. The absolute concentration method was used for drug susceptibility testing. Mutations katG, inhA and rpoB associated with resistance to isoniazid and rifampicin were detected by microchip technology («TB-Biochip»). The isolates were genotyped by real-time PCR for the detection of Beijing/non-Beijing genotypes and 15-locus MIRU-VNTR typing according to «MIRU-VNTRplus» (http://www.miru-vntrplus.org). More than half (55.1%) of 178 isolates belonged to the Beijing family, 58.7% of them were multiple drug resistant (MDR) mostly due to rpoBSer531→Leu and katGSer315→Thr1 substitutions. Fifty VNTR profiles were found in 98 Beijing isolates; 57 of them grouped into 9 clusters. The largest VNTR cluster included 23 (23.5%) Beijing isolates and 21 of them were MDR. The 80 non-Beijing isolates showed 64 distinct VNTR patterns which belonged to 6 genetic families: LAM, Ural, Haarlem, etc. Among LAM and Ural isolates 30.4% and 28.6% were MDR, respectively. The 5 of 7 MDR LAM isolates had specific mutation profile: rpoBAsp516→Val substitution and mutations katGSer315→Thr1 and inhA_T15. The MDR Ural isolates showed the heterogeneity of mutations in rpoB gene compared to other genotypes. Taken together, these findings suggest the emergence and spread of MDR-TB in the Ural region which is characterized by circulation of MDR strains of different genotypes with the Beijing family genotype to be predominant.T. V. UmpelevaM. A. KravchenkoN. I. EremeevaA. A. VyazovayaO. V. NarvskayaSankt-Peterburg : NIIÈM imeni Pasteraarticlemycobacterium tuberculosisgenotypingvntrtb-biochipInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 3, Iss 1, Pp 21-28 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	mycobacterium tuberculosis genotyping vntr tb-biochip Infectious and parasitic diseases RC109-216
spellingShingle	mycobacterium tuberculosis genotyping vntr tb-biochip Infectious and parasitic diseases RC109-216 T. V. Umpeleva M. A. Kravchenko N. I. Eremeeva A. A. Vyazovaya O. V. Narvskaya MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS STRAINS CIRCULATING IN THE URAL REGION, RUSSIA
description	Abstract. Overall 178 Mycobacterium tuberculosis isolates recovered in 2009–2011 from newly diagnosed epidemiologically unlinked to TB patients in the Ural region of Russia have been studied. The absolute concentration method was used for drug susceptibility testing. Mutations katG, inhA and rpoB associated with resistance to isoniazid and rifampicin were detected by microchip technology («TB-Biochip»). The isolates were genotyped by real-time PCR for the detection of Beijing/non-Beijing genotypes and 15-locus MIRU-VNTR typing according to «MIRU-VNTRplus» (http://www.miru-vntrplus.org). More than half (55.1%) of 178 isolates belonged to the Beijing family, 58.7% of them were multiple drug resistant (MDR) mostly due to rpoBSer531→Leu and katGSer315→Thr1 substitutions. Fifty VNTR profiles were found in 98 Beijing isolates; 57 of them grouped into 9 clusters. The largest VNTR cluster included 23 (23.5%) Beijing isolates and 21 of them were MDR. The 80 non-Beijing isolates showed 64 distinct VNTR patterns which belonged to 6 genetic families: LAM, Ural, Haarlem, etc. Among LAM and Ural isolates 30.4% and 28.6% were MDR, respectively. The 5 of 7 MDR LAM isolates had specific mutation profile: rpoBAsp516→Val substitution and mutations katGSer315→Thr1 and inhA_T15. The MDR Ural isolates showed the heterogeneity of mutations in rpoB gene compared to other genotypes. Taken together, these findings suggest the emergence and spread of MDR-TB in the Ural region which is characterized by circulation of MDR strains of different genotypes with the Beijing family genotype to be predominant.
format	article
author	T. V. Umpeleva M. A. Kravchenko N. I. Eremeeva A. A. Vyazovaya O. V. Narvskaya
author_facet	T. V. Umpeleva M. A. Kravchenko N. I. Eremeeva A. A. Vyazovaya O. V. Narvskaya
author_sort	T. V. Umpeleva
title	MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS STRAINS CIRCULATING IN THE URAL REGION, RUSSIA
title_short	MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS STRAINS CIRCULATING IN THE URAL REGION, RUSSIA
title_full	MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS STRAINS CIRCULATING IN THE URAL REGION, RUSSIA
title_fullStr	MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS STRAINS CIRCULATING IN THE URAL REGION, RUSSIA
title_full_unstemmed	MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS STRAINS CIRCULATING IN THE URAL REGION, RUSSIA
title_sort	molecular characterization of mycobacterium tuberculosis strains circulating in the ural region, russia
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2014
url	https://doaj.org/article/5333b9715def421caa5a6b99f9521789
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MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM TUBERCULOSIS STRAINS CIRCULATING IN THE URAL REGION, RUSSIA

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