Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET

Cellular signaling processes often involve trafficking of receptors and other proteins between subcellular compartments. Here the authors demonstrate a method based on the concept of Enhanced bystander Bioluminescence Resonance Energy Transfer (EbBRET) that allows efficient real time monitoring of e...

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Autores principales: Yoon Namkung, Christian Le Gouill, Viktoria Lukashova, Hiroyuki Kobayashi, Mireille Hogue, Etienne Khoury, Mideum Song, Michel Bouvier, Stéphane A. Laporte
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/533dd58c08184acea1bdb815fa853d15
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spelling oai:doaj.org-article:533dd58c08184acea1bdb815fa853d152021-12-02T15:35:43ZMonitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET10.1038/ncomms121782041-1723https://doaj.org/article/533dd58c08184acea1bdb815fa853d152016-07-01T00:00:00Zhttps://doi.org/10.1038/ncomms12178https://doaj.org/toc/2041-1723Cellular signaling processes often involve trafficking of receptors and other proteins between subcellular compartments. Here the authors demonstrate a method based on the concept of Enhanced bystander Bioluminescence Resonance Energy Transfer (EbBRET) that allows efficient real time monitoring of endocytosis and trafficking.Yoon NamkungChristian Le GouillViktoria LukashovaHiroyuki KobayashiMireille HogueEtienne KhouryMideum SongMichel BouvierStéphane A. LaporteNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-12 (2016)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Yoon Namkung
Christian Le Gouill
Viktoria Lukashova
Hiroyuki Kobayashi
Mireille Hogue
Etienne Khoury
Mideum Song
Michel Bouvier
Stéphane A. Laporte
Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET
description Cellular signaling processes often involve trafficking of receptors and other proteins between subcellular compartments. Here the authors demonstrate a method based on the concept of Enhanced bystander Bioluminescence Resonance Energy Transfer (EbBRET) that allows efficient real time monitoring of endocytosis and trafficking.
format article
author Yoon Namkung
Christian Le Gouill
Viktoria Lukashova
Hiroyuki Kobayashi
Mireille Hogue
Etienne Khoury
Mideum Song
Michel Bouvier
Stéphane A. Laporte
author_facet Yoon Namkung
Christian Le Gouill
Viktoria Lukashova
Hiroyuki Kobayashi
Mireille Hogue
Etienne Khoury
Mideum Song
Michel Bouvier
Stéphane A. Laporte
author_sort Yoon Namkung
title Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET
title_short Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET
title_full Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET
title_fullStr Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET
title_full_unstemmed Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET
title_sort monitoring g protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander bret
publisher Nature Portfolio
publishDate 2016
url https://doaj.org/article/533dd58c08184acea1bdb815fa853d15
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