High-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs

Abstract Non-alcoholic fatty liver disease (NAFLD) is an increasingly prevalent condition that has been linked to high-fructose corn syrup consumption with induction of hepatic de novo lipogenesis (DNL) as the suggested central mechanism. Feeding diets very high in fructose (> 60%) rapidly induce...

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Autores principales:	Nikolaj H. Schmidt, Pia Svendsen, Julián Albarrán-Juárez, Søren K. Moestrup, Jacob Fog Bentzon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5346f183c93a46909dba4a391bdd018c
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spelling	oai:doaj.org-article:5346f183c93a46909dba4a391bdd018c2021-12-02T14:06:31ZHigh-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs10.1038/s41598-021-82208-12045-2322https://doaj.org/article/5346f183c93a46909dba4a391bdd018c2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82208-1https://doaj.org/toc/2045-2322Abstract Non-alcoholic fatty liver disease (NAFLD) is an increasingly prevalent condition that has been linked to high-fructose corn syrup consumption with induction of hepatic de novo lipogenesis (DNL) as the suggested central mechanism. Feeding diets very high in fructose (> 60%) rapidly induce several features of NAFLD in rodents, but similar diets have not yet been applied in larger animals, such as pigs. With the aim to develop a large animal NAFLD model, we analysed the effects of feeding a high-fructose (HF, 60% w/w) diet for four weeks to castrated male Danish Landrace-York-Duroc pigs. HF feeding upregulated expression of hepatic DNL proteins, but levels were low compared with adipose tissue. No steatosis or hepatocellular ballooning was seen on histopathological examination, and plasma levels of transaminases were similar between groups. Inflammatory infiltrates and the amount of connective tissue was slightly elevated in liver sections from fructose-fed pigs, which was corroborated by up-regulation of macrophage marker expression in liver homogenates. Supported by RNA-profiling, quantitative protein analysis, histopathological examination, and biochemistry, our data suggest that pigs, contrary to rodents and humans, are protected against fructose-induced steatosis by relying on adipose tissue rather than liver for DNL.Nikolaj H. SchmidtPia SvendsenJulián Albarrán-JuárezSøren K. MoestrupJacob Fog BentzonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nikolaj H. Schmidt Pia Svendsen Julián Albarrán-Juárez Søren K. Moestrup Jacob Fog Bentzon High-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs
description	Abstract Non-alcoholic fatty liver disease (NAFLD) is an increasingly prevalent condition that has been linked to high-fructose corn syrup consumption with induction of hepatic de novo lipogenesis (DNL) as the suggested central mechanism. Feeding diets very high in fructose (> 60%) rapidly induce several features of NAFLD in rodents, but similar diets have not yet been applied in larger animals, such as pigs. With the aim to develop a large animal NAFLD model, we analysed the effects of feeding a high-fructose (HF, 60% w/w) diet for four weeks to castrated male Danish Landrace-York-Duroc pigs. HF feeding upregulated expression of hepatic DNL proteins, but levels were low compared with adipose tissue. No steatosis or hepatocellular ballooning was seen on histopathological examination, and plasma levels of transaminases were similar between groups. Inflammatory infiltrates and the amount of connective tissue was slightly elevated in liver sections from fructose-fed pigs, which was corroborated by up-regulation of macrophage marker expression in liver homogenates. Supported by RNA-profiling, quantitative protein analysis, histopathological examination, and biochemistry, our data suggest that pigs, contrary to rodents and humans, are protected against fructose-induced steatosis by relying on adipose tissue rather than liver for DNL.
format	article
author	Nikolaj H. Schmidt Pia Svendsen Julián Albarrán-Juárez Søren K. Moestrup Jacob Fog Bentzon
author_facet	Nikolaj H. Schmidt Pia Svendsen Julián Albarrán-Juárez Søren K. Moestrup Jacob Fog Bentzon
author_sort	Nikolaj H. Schmidt
title	High-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs
title_short	High-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs
title_full	High-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs
title_fullStr	High-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs
title_full_unstemmed	High-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs
title_sort	high-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5346f183c93a46909dba4a391bdd018c
work_keys_str_mv	AT nikolajhschmidt highfructosefeedingdoesnotinducesteatosisornonalcoholicfattyliverdiseaseinpigs AT piasvendsen highfructosefeedingdoesnotinducesteatosisornonalcoholicfattyliverdiseaseinpigs AT julianalbarranjuarez highfructosefeedingdoesnotinducesteatosisornonalcoholicfattyliverdiseaseinpigs AT sørenkmoestrup highfructosefeedingdoesnotinducesteatosisornonalcoholicfattyliverdiseaseinpigs AT jacobfogbentzon highfructosefeedingdoesnotinducesteatosisornonalcoholicfattyliverdiseaseinpigs
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High-fructose feeding does not induce steatosis or non-alcoholic fatty liver disease in pigs

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