In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress

Abstract Drought-induced water stress affects the productivity of the Vanilla planifolia Jacks. ex Andrews crop. In vitro culture technique is an effective tool for the study of water stress tolerance mechanisms. This study aimed to evaluate the morphological, physiological and biochemical response...

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Autores principales:	Eduardo Martínez-Santos, Carlos Alberto Cruz-Cruz, José Luis Spinoso-Castillo, Jericó Jabín Bello-Bello
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/534aa887a4974ceea43dea63dac9038d
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spelling	oai:doaj.org-article:534aa887a4974ceea43dea63dac9038d2021-11-21T12:18:50ZIn vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress10.1038/s41598-021-02207-02045-2322https://doaj.org/article/534aa887a4974ceea43dea63dac9038d2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02207-0https://doaj.org/toc/2045-2322Abstract Drought-induced water stress affects the productivity of the Vanilla planifolia Jacks. ex Andrews crop. In vitro culture technique is an effective tool for the study of water stress tolerance mechanisms. This study aimed to evaluate the morphological, physiological and biochemical response of V. planifolia under in vitro water stress conditions induced with polyethylene glycol (PEG). In vitro regenerated shoots of 2 cm in length were subjected to different concentrations of PEG 6000 (0, 1, 2 and 3% w/v) using Murashige and Skoog semi-solid culture medium. At 60 days of culture, different growth variables, dry matter (DM) content, chlorophyll (Chl), soluble proteins (SP), proline (Pro), glycine betaine (GB), stomatal index (SI) and open stomata (%) were evaluated. Results showed a reduction in growth, Chl content, SP, SI and open stomata (%) with increasing PEG concentration, whereas DM, Pro and GB contents rose with increasing PEG concentration. In conclusion, PEG-induced osmotic stress allowed describing physiological and biochemical mechanisms of response to water stress. Furthermore, the determination of compatible Pro and GB osmolytes can be used as biochemical markers in future breeding programs for the early selection of water stress tolerant genotypes.Eduardo Martínez-SantosCarlos Alberto Cruz-CruzJosé Luis Spinoso-CastilloJericó Jabín Bello-BelloNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eduardo Martínez-Santos Carlos Alberto Cruz-Cruz José Luis Spinoso-Castillo Jericó Jabín Bello-Bello In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress
description	Abstract Drought-induced water stress affects the productivity of the Vanilla planifolia Jacks. ex Andrews crop. In vitro culture technique is an effective tool for the study of water stress tolerance mechanisms. This study aimed to evaluate the morphological, physiological and biochemical response of V. planifolia under in vitro water stress conditions induced with polyethylene glycol (PEG). In vitro regenerated shoots of 2 cm in length were subjected to different concentrations of PEG 6000 (0, 1, 2 and 3% w/v) using Murashige and Skoog semi-solid culture medium. At 60 days of culture, different growth variables, dry matter (DM) content, chlorophyll (Chl), soluble proteins (SP), proline (Pro), glycine betaine (GB), stomatal index (SI) and open stomata (%) were evaluated. Results showed a reduction in growth, Chl content, SP, SI and open stomata (%) with increasing PEG concentration, whereas DM, Pro and GB contents rose with increasing PEG concentration. In conclusion, PEG-induced osmotic stress allowed describing physiological and biochemical mechanisms of response to water stress. Furthermore, the determination of compatible Pro and GB osmolytes can be used as biochemical markers in future breeding programs for the early selection of water stress tolerant genotypes.
format	article
author	Eduardo Martínez-Santos Carlos Alberto Cruz-Cruz José Luis Spinoso-Castillo Jericó Jabín Bello-Bello
author_facet	Eduardo Martínez-Santos Carlos Alberto Cruz-Cruz José Luis Spinoso-Castillo Jericó Jabín Bello-Bello
author_sort	Eduardo Martínez-Santos
title	In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress
title_short	In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress
title_full	In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress
title_fullStr	In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress
title_full_unstemmed	In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress
title_sort	in vitro response of vanilla (vanilla planifolia jacks. ex andrews) to peg-induced osmotic stress
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/534aa887a4974ceea43dea63dac9038d
work_keys_str_mv	AT eduardomartinezsantos invitroresponseofvanillavanillaplanifoliajacksexandrewstopeginducedosmoticstress AT carlosalbertocruzcruz invitroresponseofvanillavanillaplanifoliajacksexandrewstopeginducedosmoticstress AT joseluisspinosocastillo invitroresponseofvanillavanillaplanifoliajacksexandrewstopeginducedosmoticstress AT jericojabinbellobello invitroresponseofvanillavanillaplanifoliajacksexandrewstopeginducedosmoticstress
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In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress

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