Proteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans

Invasive pulmonary aspergillosis (IPA) is a severe infection that is difficult to diagnose due to the ubiquitous presence of fungal spores, the underlying diseases of risk patients, and limitations of currently available markers. In this study, we performed a comprehensive liquid chromatography tand...

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Autores principales:	Silke Machata, Mario M. Müller, Roland Lehmann, Patricia Sieber, Gianni Panagiotou, Agostinho Carvalho, Cristina Cunha, Katrien Lagrou, Johan Maertens, Hortense Slevogt, Ilse D. Jacobsen
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	invasive aspergillosis fungal pulmonary infection aspergillus fumigatus biomarker proteome analysis Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/5350df04459a440e9ade50dfa1dcae57
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spelling	oai:doaj.org-article:5350df04459a440e9ade50dfa1dcae572021-11-17T14:21:59ZProteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans2150-55942150-560810.1080/21505594.2020.1824960https://doaj.org/article/5350df04459a440e9ade50dfa1dcae572020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1824960https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Invasive pulmonary aspergillosis (IPA) is a severe infection that is difficult to diagnose due to the ubiquitous presence of fungal spores, the underlying diseases of risk patients, and limitations of currently available markers. In this study, we performed a comprehensive liquid chromatography tandem mass spectrometry (LC-MS/MS)-based identification of host and fungal proteins expressed during IPA in mice and humans. The proteomic analysis of bronchoalveolar lavage samples of individual IPA and control cases allowed the description of common host factors that had significantly increased abundance in both infected animals and IPA patients compared to their controls. Although increased levels of these individual host proteins might not be sufficient to distinguish bacterial from fungal infection, a combination of these markers might be beneficial to improve diagnosis. We also identified 16 fungal proteins that were specifically detected during infection and may be valuable candidates for biomarker evaluation.Silke MachataMario M. MüllerRoland LehmannPatricia SieberGianni PanagiotouAgostinho CarvalhoCristina CunhaKatrien LagrouJohan MaertensHortense SlevogtIlse D. JacobsenTaylor & Francis Grouparticleinvasive aspergillosisfungal pulmonary infectionaspergillus fumigatusbiomarkerproteome analysisInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1337-1351 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	invasive aspergillosis fungal pulmonary infection aspergillus fumigatus biomarker proteome analysis Infectious and parasitic diseases RC109-216
spellingShingle	invasive aspergillosis fungal pulmonary infection aspergillus fumigatus biomarker proteome analysis Infectious and parasitic diseases RC109-216 Silke Machata Mario M. Müller Roland Lehmann Patricia Sieber Gianni Panagiotou Agostinho Carvalho Cristina Cunha Katrien Lagrou Johan Maertens Hortense Slevogt Ilse D. Jacobsen Proteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans
description	Invasive pulmonary aspergillosis (IPA) is a severe infection that is difficult to diagnose due to the ubiquitous presence of fungal spores, the underlying diseases of risk patients, and limitations of currently available markers. In this study, we performed a comprehensive liquid chromatography tandem mass spectrometry (LC-MS/MS)-based identification of host and fungal proteins expressed during IPA in mice and humans. The proteomic analysis of bronchoalveolar lavage samples of individual IPA and control cases allowed the description of common host factors that had significantly increased abundance in both infected animals and IPA patients compared to their controls. Although increased levels of these individual host proteins might not be sufficient to distinguish bacterial from fungal infection, a combination of these markers might be beneficial to improve diagnosis. We also identified 16 fungal proteins that were specifically detected during infection and may be valuable candidates for biomarker evaluation.
format	article
author	Silke Machata Mario M. Müller Roland Lehmann Patricia Sieber Gianni Panagiotou Agostinho Carvalho Cristina Cunha Katrien Lagrou Johan Maertens Hortense Slevogt Ilse D. Jacobsen
author_facet	Silke Machata Mario M. Müller Roland Lehmann Patricia Sieber Gianni Panagiotou Agostinho Carvalho Cristina Cunha Katrien Lagrou Johan Maertens Hortense Slevogt Ilse D. Jacobsen
author_sort	Silke Machata
title	Proteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans
title_short	Proteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans
title_full	Proteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans
title_fullStr	Proteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans
title_full_unstemmed	Proteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans
title_sort	proteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/5350df04459a440e9ade50dfa1dcae57
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Proteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans

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