Optical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease

Abstract This study tested the utility of optical coherence tomography (OCT)-based indentation to assess mechanical properties of respiratory tissues in disease. Using OCT-based indentation, the elastic modulus of mouse diaphragm was measured from changes in diaphragm thickness in response to an app...

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Autores principales:	Kimberley C. W. Wang, Chrissie J. Astell, Philip Wijesinghe, Alexander N. Larcombe, Gavin J. Pinniger, Graeme R. Zosky, Brendan F. Kennedy, Luke J. Berry, David D. Sampson, Alan L. James, Timothy D. Le Cras, Peter B. Noble
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/53699387192b42dea72b5840ddd0edfd
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spelling	oai:doaj.org-article:53699387192b42dea72b5840ddd0edfd2021-12-02T15:06:13ZOptical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease10.1038/s41598-017-01431-x2045-2322https://doaj.org/article/53699387192b42dea72b5840ddd0edfd2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01431-xhttps://doaj.org/toc/2045-2322Abstract This study tested the utility of optical coherence tomography (OCT)-based indentation to assess mechanical properties of respiratory tissues in disease. Using OCT-based indentation, the elastic modulus of mouse diaphragm was measured from changes in diaphragm thickness in response to an applied force provided by an indenter. We used a transgenic mouse model of chronic lung disease induced by the overexpression of transforming growth factor-alpha (TGF-α), established by the presence of pleural and peribronchial fibrosis and impaired lung mechanics determined by the forced oscillation technique and plethysmography. Diaphragm elastic modulus assessed by OCT-based indentation was reduced by TGF-α at both left and right lateral locations (p < 0.05). Diaphragm elastic modulus at left and right lateral locations were correlated within mice (r = 0.67, p < 0.01) suggesting that measurements were representative of tissue beyond the indenter field. Co-localised images of diaphragm after TGF-α overexpression revealed a layered fibrotic appearance. Maximum diaphragm force in conventional organ bath studies was also reduced by TGF-α overexpression (p < 0.01). Results show that OCT-based indentation provided clear delineation of diseased diaphragm, and together with organ bath assessment, provides new evidence suggesting that TGF-α overexpression produces impairment in diaphragm function and, therefore, an increase in the work of breathing in chronic lung disease.Kimberley C. W. WangChrissie J. AstellPhilip WijesingheAlexander N. LarcombeGavin J. PinnigerGraeme R. ZoskyBrendan F. KennedyLuke J. BerryDavid D. SampsonAlan L. JamesTimothy D. Le CrasPeter B. NobleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kimberley C. W. Wang Chrissie J. Astell Philip Wijesinghe Alexander N. Larcombe Gavin J. Pinniger Graeme R. Zosky Brendan F. Kennedy Luke J. Berry David D. Sampson Alan L. James Timothy D. Le Cras Peter B. Noble Optical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease
description	Abstract This study tested the utility of optical coherence tomography (OCT)-based indentation to assess mechanical properties of respiratory tissues in disease. Using OCT-based indentation, the elastic modulus of mouse diaphragm was measured from changes in diaphragm thickness in response to an applied force provided by an indenter. We used a transgenic mouse model of chronic lung disease induced by the overexpression of transforming growth factor-alpha (TGF-α), established by the presence of pleural and peribronchial fibrosis and impaired lung mechanics determined by the forced oscillation technique and plethysmography. Diaphragm elastic modulus assessed by OCT-based indentation was reduced by TGF-α at both left and right lateral locations (p < 0.05). Diaphragm elastic modulus at left and right lateral locations were correlated within mice (r = 0.67, p < 0.01) suggesting that measurements were representative of tissue beyond the indenter field. Co-localised images of diaphragm after TGF-α overexpression revealed a layered fibrotic appearance. Maximum diaphragm force in conventional organ bath studies was also reduced by TGF-α overexpression (p < 0.01). Results show that OCT-based indentation provided clear delineation of diseased diaphragm, and together with organ bath assessment, provides new evidence suggesting that TGF-α overexpression produces impairment in diaphragm function and, therefore, an increase in the work of breathing in chronic lung disease.
format	article
author	Kimberley C. W. Wang Chrissie J. Astell Philip Wijesinghe Alexander N. Larcombe Gavin J. Pinniger Graeme R. Zosky Brendan F. Kennedy Luke J. Berry David D. Sampson Alan L. James Timothy D. Le Cras Peter B. Noble
author_facet	Kimberley C. W. Wang Chrissie J. Astell Philip Wijesinghe Alexander N. Larcombe Gavin J. Pinniger Graeme R. Zosky Brendan F. Kennedy Luke J. Berry David D. Sampson Alan L. James Timothy D. Le Cras Peter B. Noble
author_sort	Kimberley C. W. Wang
title	Optical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease
title_short	Optical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease
title_full	Optical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease
title_fullStr	Optical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease
title_full_unstemmed	Optical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease
title_sort	optical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/53699387192b42dea72b5840ddd0edfd
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Optical coherence tomography-based contact indentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease

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