Human mesenchymal stem cell-derived iron oxide exosomes allow targeted ablation of tumor cells via magnetic hyperthermia

U Altanerova,1 M Babincova,2 P Babinec,2 K Benejova,1 J Jakubechova,1 V Altanerova,1 M Zduriencikova,3 V Repiska,4 C Altaner1,3 1Stem Cell Preparation Department, St Elisabeth Cancer Institute, Bratislava, Slovakia; 2Department of Nuclear Physics and Biophysics, Comenius University, Bratislava, Slo...

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Autores principales:	Altanerova U, Babincova M, Babinec P, Benejova K, Jakubechova J, Altanerova V, Zduriencikova M, Repiska V, Altaner C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	mesenchymal stem cells iron oxide labeling Venofer yCD::UPRT-exosomes yCD::UPRT-MSCs/Fe exosomes magnetic hyperthermia Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/536b6f7b259a4d5b860c2c9e72ddf005
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spelling	oai:doaj.org-article:536b6f7b259a4d5b860c2c9e72ddf0052021-12-02T02:40:32ZHuman mesenchymal stem cell-derived iron oxide exosomes allow targeted ablation of tumor cells via magnetic hyperthermia1178-2013https://doaj.org/article/536b6f7b259a4d5b860c2c9e72ddf0052017-10-01T00:00:00Zhttps://www.dovepress.com/human-mesenchymal-stem-cell-derived-iron-oxide-exosomes-allow-targeted-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013U Altanerova,1 M Babincova,2 P Babinec,2 K Benejova,1 J Jakubechova,1 V Altanerova,1 M Zduriencikova,3 V Repiska,4 C Altaner1,3 1Stem Cell Preparation Department, St Elisabeth Cancer Institute, Bratislava, Slovakia; 2Department of Nuclear Physics and Biophysics, Comenius University, Bratislava, Slovakia; 3Cancer Research Institute, Biomedical Center, Slovak Academy of Sciences, Bratislava, Slovakia; 4Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Slovakia Abstract: Magnetic hyperthermia, or the heating of tissues using magnetic materials, is a promising approach for treating cancer. We found that human mesenchymal stem cells (MSCs) isolated from various tissues and MSCs expressing the yeast cytosine deaminase::uracil phosphoribosyl transferase suicide fusion gene (yCD::UPRT) can be labeled with Venofer, an iron oxide carbohydrate nanoparticle. Venofer labeling did not affect cell proliferation or the ability to home to tumors. All Venofer-labeled MSCs released exosomes that contained iron oxide. Furthermore, these exosomes were efficiently endocytosed by tumor cells. Exosomes from Venofer-labeled MSCs expressing the yCD::UPRT gene in the presence of the prodrug 5-fluorocytosine inhibited tumor growth in a dose-dependent fashion. The treated tumor cells were also effectively ablated following induction of hyperthermia using an external alternating magnetic field. Cumulatively, we found that magnetic nanoparticles packaged into MSC exosomes are efficiently endocytosed by tumor cells, facilitating targeted tumor cell ablation via magnetically induced hyperthermia. Keywords: mesenchymal stem cells, iron oxide labeling, Venofer, yCD::UPRT-exosomes, yCD::UPRT-MSCs/Fe exosomes, magnetic hyperthermiaAltanerova UBabincova MBabinec PBenejova KJakubechova JAltanerova VZduriencikova MRepiska VAltaner CDove Medical Pressarticlemesenchymal stem cellsiron oxide labelingVenoferyCD::UPRT-exosomesyCD::UPRT-MSCs/Fe exosomesmagnetic hyperthermiaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7923-7936 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	mesenchymal stem cells iron oxide labeling Venofer yCD::UPRT-exosomes yCD::UPRT-MSCs/Fe exosomes magnetic hyperthermia Medicine (General) R5-920
spellingShingle	mesenchymal stem cells iron oxide labeling Venofer yCD::UPRT-exosomes yCD::UPRT-MSCs/Fe exosomes magnetic hyperthermia Medicine (General) R5-920 Altanerova U Babincova M Babinec P Benejova K Jakubechova J Altanerova V Zduriencikova M Repiska V Altaner C Human mesenchymal stem cell-derived iron oxide exosomes allow targeted ablation of tumor cells via magnetic hyperthermia
description	U Altanerova,1 M Babincova,2 P Babinec,2 K Benejova,1 J Jakubechova,1 V Altanerova,1 M Zduriencikova,3 V Repiska,4 C Altaner1,3 1Stem Cell Preparation Department, St Elisabeth Cancer Institute, Bratislava, Slovakia; 2Department of Nuclear Physics and Biophysics, Comenius University, Bratislava, Slovakia; 3Cancer Research Institute, Biomedical Center, Slovak Academy of Sciences, Bratislava, Slovakia; 4Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Slovakia Abstract: Magnetic hyperthermia, or the heating of tissues using magnetic materials, is a promising approach for treating cancer. We found that human mesenchymal stem cells (MSCs) isolated from various tissues and MSCs expressing the yeast cytosine deaminase::uracil phosphoribosyl transferase suicide fusion gene (yCD::UPRT) can be labeled with Venofer, an iron oxide carbohydrate nanoparticle. Venofer labeling did not affect cell proliferation or the ability to home to tumors. All Venofer-labeled MSCs released exosomes that contained iron oxide. Furthermore, these exosomes were efficiently endocytosed by tumor cells. Exosomes from Venofer-labeled MSCs expressing the yCD::UPRT gene in the presence of the prodrug 5-fluorocytosine inhibited tumor growth in a dose-dependent fashion. The treated tumor cells were also effectively ablated following induction of hyperthermia using an external alternating magnetic field. Cumulatively, we found that magnetic nanoparticles packaged into MSC exosomes are efficiently endocytosed by tumor cells, facilitating targeted tumor cell ablation via magnetically induced hyperthermia. Keywords: mesenchymal stem cells, iron oxide labeling, Venofer, yCD::UPRT-exosomes, yCD::UPRT-MSCs/Fe exosomes, magnetic hyperthermia
format	article
author	Altanerova U Babincova M Babinec P Benejova K Jakubechova J Altanerova V Zduriencikova M Repiska V Altaner C
author_facet	Altanerova U Babincova M Babinec P Benejova K Jakubechova J Altanerova V Zduriencikova M Repiska V Altaner C
author_sort	Altanerova U
title	Human mesenchymal stem cell-derived iron oxide exosomes allow targeted ablation of tumor cells via magnetic hyperthermia
title_short	Human mesenchymal stem cell-derived iron oxide exosomes allow targeted ablation of tumor cells via magnetic hyperthermia
title_full	Human mesenchymal stem cell-derived iron oxide exosomes allow targeted ablation of tumor cells via magnetic hyperthermia
title_fullStr	Human mesenchymal stem cell-derived iron oxide exosomes allow targeted ablation of tumor cells via magnetic hyperthermia
title_full_unstemmed	Human mesenchymal stem cell-derived iron oxide exosomes allow targeted ablation of tumor cells via magnetic hyperthermia
title_sort	human mesenchymal stem cell-derived iron oxide exosomes allow targeted ablation of tumor cells via magnetic hyperthermia
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/536b6f7b259a4d5b860c2c9e72ddf005
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Human mesenchymal stem cell-derived iron oxide exosomes allow targeted ablation of tumor cells via magnetic hyperthermia

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