Polyphenol-Conjugated Bimetallic Au@AgNPs for Improved Wound Healing

Piotr Orlowski,1 Magdalena Zmigrodzka,2 Emilia Tomaszewska,3 Katarzyna Ranoszek-Soliwoda,3 Beata Pajak,4 Anna Slonska,5 Joanna Cymerys,5 Grzegorz Celichowski,3 Jaroslaw Grobelny,3 Malgorzata Krzyzowska1 1Laboratory of Nanobiology and Biomaterials, Military Institute of Hygiene and Epidemiology, Wars...

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Autores principales:	Orlowski P, Zmigrodzka M, Tomaszewska E, Ranoszek-Soliwoda K, Pajak B, Slonska A, Cymerys J, Celichowski G, Grobelny J, Krzyzowska M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	polyphenols bimetallic nanoparticles wound healing mouse model Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/538875e22571427c9c92927f5c63789e
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spelling	oai:doaj.org-article:538875e22571427c9c92927f5c63789e2021-12-02T12:53:21ZPolyphenol-Conjugated Bimetallic Au@AgNPs for Improved Wound Healing1178-2013https://doaj.org/article/538875e22571427c9c92927f5c63789e2020-07-01T00:00:00Zhttps://www.dovepress.com/polyphenol-conjugated-bimetallic-auagnps-for-improved-wound-healing-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Piotr Orlowski,1 Magdalena Zmigrodzka,2 Emilia Tomaszewska,3 Katarzyna Ranoszek-Soliwoda,3 Beata Pajak,4 Anna Slonska,5 Joanna Cymerys,5 Grzegorz Celichowski,3 Jaroslaw Grobelny,3 Malgorzata Krzyzowska1 1Laboratory of Nanobiology and Biomaterials, Military Institute of Hygiene and Epidemiology, Warsaw, Poland; 2Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences (WULS-SGGW), Warsaw, Poland; 3Department of Materials Technology and Chemistry, Faculty of Chemistry, University of Lodz, Lodz, Poland; 4Laboratory of Genetics and Molecular Biology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland; 5Division of Microbiology, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw 02-786, PolandCorrespondence: Malgorzata Krzyzowska Email krzyzowskam@yahoo.comBackground: Polyphenols possess antioxidant, anti-inflammatory and antimicrobial properties and have been used in the treatment of skin wounds and burns. We previously showed that tannic acid-modified AgNPs sized > 26 nm promote wound healing, while tannic acid-modified AgNPs sized 13 nm can elicit strong local inflammatory response. In this study, we tested bimetallic Au@AgNPs sized 30 nm modified with selected flavonoid and non-flavonoid compounds for wound healing applications.Methods: Bimetallic Au@AgNPs were obtained by growing an Ag layer on AuNPs and further modified with selected polyphenols. After toxicity tests and in vitro scratch assay in HaCaT cells, modified lymph node assay as well as the mouse splint wound model were further used to access the wound healing potential of selected non-toxic modifications.Results: Tannic acid, gallic acid, polydatin, resveratrol, catechin, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate and procyanidin B2 used to modify Au@AgNPs exhibited good toxicological profiles in HaCaT cells. Au@AgNPs modified with 15 μM tannic acid, 200 μM resveratrol, 200 μM epicatechin gallate, 1000 μM gallic acid and 200 μM procyanidin B2 induced wound healing in vivo and did not lead to the local irritation or inflammation. Tannic acid-modified Au@AgNPs induced epithelial-to-mesenchymal transition (EMT) – like re-epithelialization, while other polyphenol modifications of Au@AgNPs acted through proliferation and wound closure.Conclusion: Bimetallic Au@AgNPs can be used as a basis for modification with selected polyphenols for topical uses. In addition, we have demonstrated that particular polyphenols used to modify bimetallic nanoparticles may show different effects upon different stages of wound healing.Keywords: polyphenols, bimetallic nanoparticles, wound healing, mouse modelOrlowski PZmigrodzka MTomaszewska ERanoszek-Soliwoda KPajak BSlonska ACymerys JCelichowski GGrobelny JKrzyzowska MDove Medical Pressarticlepolyphenolsbimetallic nanoparticleswound healingmouse modelMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 4969-4990 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyphenols bimetallic nanoparticles wound healing mouse model Medicine (General) R5-920
spellingShingle	polyphenols bimetallic nanoparticles wound healing mouse model Medicine (General) R5-920 Orlowski P Zmigrodzka M Tomaszewska E Ranoszek-Soliwoda K Pajak B Slonska A Cymerys J Celichowski G Grobelny J Krzyzowska M Polyphenol-Conjugated Bimetallic Au@AgNPs for Improved Wound Healing
description	Piotr Orlowski,1 Magdalena Zmigrodzka,2 Emilia Tomaszewska,3 Katarzyna Ranoszek-Soliwoda,3 Beata Pajak,4 Anna Slonska,5 Joanna Cymerys,5 Grzegorz Celichowski,3 Jaroslaw Grobelny,3 Malgorzata Krzyzowska1 1Laboratory of Nanobiology and Biomaterials, Military Institute of Hygiene and Epidemiology, Warsaw, Poland; 2Department of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences (WULS-SGGW), Warsaw, Poland; 3Department of Materials Technology and Chemistry, Faculty of Chemistry, University of Lodz, Lodz, Poland; 4Laboratory of Genetics and Molecular Biology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland; 5Division of Microbiology, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw 02-786, PolandCorrespondence: Malgorzata Krzyzowska Email krzyzowskam@yahoo.comBackground: Polyphenols possess antioxidant, anti-inflammatory and antimicrobial properties and have been used in the treatment of skin wounds and burns. We previously showed that tannic acid-modified AgNPs sized > 26 nm promote wound healing, while tannic acid-modified AgNPs sized 13 nm can elicit strong local inflammatory response. In this study, we tested bimetallic Au@AgNPs sized 30 nm modified with selected flavonoid and non-flavonoid compounds for wound healing applications.Methods: Bimetallic Au@AgNPs were obtained by growing an Ag layer on AuNPs and further modified with selected polyphenols. After toxicity tests and in vitro scratch assay in HaCaT cells, modified lymph node assay as well as the mouse splint wound model were further used to access the wound healing potential of selected non-toxic modifications.Results: Tannic acid, gallic acid, polydatin, resveratrol, catechin, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate and procyanidin B2 used to modify Au@AgNPs exhibited good toxicological profiles in HaCaT cells. Au@AgNPs modified with 15 μM tannic acid, 200 μM resveratrol, 200 μM epicatechin gallate, 1000 μM gallic acid and 200 μM procyanidin B2 induced wound healing in vivo and did not lead to the local irritation or inflammation. Tannic acid-modified Au@AgNPs induced epithelial-to-mesenchymal transition (EMT) – like re-epithelialization, while other polyphenol modifications of Au@AgNPs acted through proliferation and wound closure.Conclusion: Bimetallic Au@AgNPs can be used as a basis for modification with selected polyphenols for topical uses. In addition, we have demonstrated that particular polyphenols used to modify bimetallic nanoparticles may show different effects upon different stages of wound healing.Keywords: polyphenols, bimetallic nanoparticles, wound healing, mouse model
format	article
author	Orlowski P Zmigrodzka M Tomaszewska E Ranoszek-Soliwoda K Pajak B Slonska A Cymerys J Celichowski G Grobelny J Krzyzowska M
author_facet	Orlowski P Zmigrodzka M Tomaszewska E Ranoszek-Soliwoda K Pajak B Slonska A Cymerys J Celichowski G Grobelny J Krzyzowska M
author_sort	Orlowski P
title	Polyphenol-Conjugated Bimetallic Au@AgNPs for Improved Wound Healing
title_short	Polyphenol-Conjugated Bimetallic Au@AgNPs for Improved Wound Healing
title_full	Polyphenol-Conjugated Bimetallic Au@AgNPs for Improved Wound Healing
title_fullStr	Polyphenol-Conjugated Bimetallic Au@AgNPs for Improved Wound Healing
title_full_unstemmed	Polyphenol-Conjugated Bimetallic Au@AgNPs for Improved Wound Healing
title_sort	polyphenol-conjugated bimetallic au@agnps for improved wound healing
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/538875e22571427c9c92927f5c63789e
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Polyphenol-Conjugated Bimetallic Au@AgNPs for Improved Wound Healing

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