Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats

Eman Ibrahim Hassanen,1 Abdelazeem Ali Khalaf,2 Adel Fathy Tohamy,2 Eman Ragab Mohammed,3 Khaled Yehia Farroh41Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; 2Department of Toxicology & Forensic Medicine, Faculty of Veterinary Medicine, Cairo Universi...

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Autores principales:	Hassanen EI, Khalaf AA, Tohamy AF, Mohammed ER, Farroh KY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Histopathology Oxidative stress Immunotoxicity Chitosan-coated silver nanoparticles Apoptosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5389aad06a7f49a1a4810546c911c05d
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spelling	oai:doaj.org-article:5389aad06a7f49a1a4810546c911c05d2021-12-02T09:32:15ZToxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats1178-2013https://doaj.org/article/5389aad06a7f49a1a4810546c911c05d2019-07-01T00:00:00Zhttps://www.dovepress.com/toxicopathological-and-immunological-studies-on-different-concentratio-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Eman Ibrahim Hassanen,1 Abdelazeem Ali Khalaf,2 Adel Fathy Tohamy,2 Eman Ragab Mohammed,3 Khaled Yehia Farroh41Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; 2Department of Toxicology & Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; 3Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; 4Nanotechnology & Advanced Materials Central Laboratory, Agricultural Research Center, Giza, EgyptBackground: Much consideration has been paid to the toxicological assessment of nanoparticles prior to clinical and biological applications. While in vitro studies have been expanding continually, in vivo investigations of nanoparticles have not developed a cohesive structure. This study aimed to assess the acute toxicity of different concentrations of chitosan-coated silver nanoparticles (Ch-AgNPs) in main organs, including liver, kidneys, and spleen.Materials and methods: Twenty-eight male albino rats were used and divided into 4 groups (n=7). Group 1 was kept as a negative control group. Groups 2, 3, and 4 were treated intraperitoneally with Ch-AgNPs each day for 14 days at doses of 50, 25, and 10 mg/kg body weight (bwt) respectively. Histopathological, morphometric and immunohistochemical studies were performed as well as oxidative stress evaluations, and specific functional examinations for each organ were elucidated.Results: It was revealed that Ch-AgNPs induced dose-dependent toxicity, and the repeated dosing of rats with 50 mg/kg Ch-AgNPs induced severe toxicities. Histopathological examination showed congestion, hemorrhage, cellular degeneration, apoptosis and necrosis in hepatic and renal tissue as well as lymphocytic depletion with increasing tangible macrophages in the spleen. The highest levels of malondialdehyde, alanine aminotransferase, aspartate aminotransferase (MDA, ALT, AST) and the lowest levels of reduced glutathione, immunoglobulin G, M and total protein (GSH, IgG, IgM, TP) were observed in this group. On the other hand, repeated dosing with 25 mg/kg induced mild to moderate disturbance in the previous parameters, while there was no significant difference in results of pathological examination and biochemical tests between the control group and those treated with 10 mg/kg bwt Ch-AgNPs.Conclusion: Chitosan-coated silver nanoparticles induce dose-dependent adverse effects on rats.Keywords: histopathology, oxidative stress, immunotoxicity, chitosan-coated silver nanoparticles, apoptosisHassanen EIKhalaf AATohamy AFMohammed ERFarroh KYDove Medical PressarticleHistopathologyOxidative stressImmunotoxicityChitosan-coated silver nanoparticlesApoptosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4723-4739 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Histopathology Oxidative stress Immunotoxicity Chitosan-coated silver nanoparticles Apoptosis Medicine (General) R5-920
spellingShingle	Histopathology Oxidative stress Immunotoxicity Chitosan-coated silver nanoparticles Apoptosis Medicine (General) R5-920 Hassanen EI Khalaf AA Tohamy AF Mohammed ER Farroh KY Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats
description	Eman Ibrahim Hassanen,1 Abdelazeem Ali Khalaf,2 Adel Fathy Tohamy,2 Eman Ragab Mohammed,3 Khaled Yehia Farroh41Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; 2Department of Toxicology & Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; 3Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; 4Nanotechnology & Advanced Materials Central Laboratory, Agricultural Research Center, Giza, EgyptBackground: Much consideration has been paid to the toxicological assessment of nanoparticles prior to clinical and biological applications. While in vitro studies have been expanding continually, in vivo investigations of nanoparticles have not developed a cohesive structure. This study aimed to assess the acute toxicity of different concentrations of chitosan-coated silver nanoparticles (Ch-AgNPs) in main organs, including liver, kidneys, and spleen.Materials and methods: Twenty-eight male albino rats were used and divided into 4 groups (n=7). Group 1 was kept as a negative control group. Groups 2, 3, and 4 were treated intraperitoneally with Ch-AgNPs each day for 14 days at doses of 50, 25, and 10 mg/kg body weight (bwt) respectively. Histopathological, morphometric and immunohistochemical studies were performed as well as oxidative stress evaluations, and specific functional examinations for each organ were elucidated.Results: It was revealed that Ch-AgNPs induced dose-dependent toxicity, and the repeated dosing of rats with 50 mg/kg Ch-AgNPs induced severe toxicities. Histopathological examination showed congestion, hemorrhage, cellular degeneration, apoptosis and necrosis in hepatic and renal tissue as well as lymphocytic depletion with increasing tangible macrophages in the spleen. The highest levels of malondialdehyde, alanine aminotransferase, aspartate aminotransferase (MDA, ALT, AST) and the lowest levels of reduced glutathione, immunoglobulin G, M and total protein (GSH, IgG, IgM, TP) were observed in this group. On the other hand, repeated dosing with 25 mg/kg induced mild to moderate disturbance in the previous parameters, while there was no significant difference in results of pathological examination and biochemical tests between the control group and those treated with 10 mg/kg bwt Ch-AgNPs.Conclusion: Chitosan-coated silver nanoparticles induce dose-dependent adverse effects on rats.Keywords: histopathology, oxidative stress, immunotoxicity, chitosan-coated silver nanoparticles, apoptosis
format	article
author	Hassanen EI Khalaf AA Tohamy AF Mohammed ER Farroh KY
author_facet	Hassanen EI Khalaf AA Tohamy AF Mohammed ER Farroh KY
author_sort	Hassanen EI
title	Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats
title_short	Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats
title_full	Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats
title_fullStr	Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats
title_full_unstemmed	Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats
title_sort	toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/5389aad06a7f49a1a4810546c911c05d
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Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats

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