Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies.

Current metabarcoding studies aiming to characterize microbial communities generally rely on the amplification and sequencing of relatively short DNA regions. For fungi, the internal transcribed spacer (ITS) region in the ribosomal RNA (rRNA) operon has been accepted as the formal fungal barcode. De...

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Autores principales: Michiel Op De Beeck, Bart Lievens, Pieter Busschaert, Stéphan Declerck, Jaco Vangronsveld, Jan V Colpaert
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
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Acceso en línea:https://doaj.org/article/538a1695feb346d6b54ba2614f192195
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