Intracellular localization of the mycobacterial stressosome complex

Abstract Microorganisms survive stresses by alternating the expression of genes suitable for surviving the immediate and present danger and eventually adapt to new conditions. Many bacteria have evolved a multiprotein "molecular machinery" designated the "Stressosome" that integr...

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Autores principales:	Malavika Ramesh, Ram Gopal Nitharwal, Phani Rama Krishna Behra, B. M. Fredrik Pettersson, Santanu Dasgupta, Leif A. Kirsebom
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/539de5fd9708428cb5bd2e1d4946e45e
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spelling	oai:doaj.org-article:539de5fd9708428cb5bd2e1d4946e45e2021-12-02T15:54:45ZIntracellular localization of the mycobacterial stressosome complex10.1038/s41598-021-89069-82045-2322https://doaj.org/article/539de5fd9708428cb5bd2e1d4946e45e2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89069-8https://doaj.org/toc/2045-2322Abstract Microorganisms survive stresses by alternating the expression of genes suitable for surviving the immediate and present danger and eventually adapt to new conditions. Many bacteria have evolved a multiprotein "molecular machinery" designated the "Stressosome" that integrates different stress signals and activates alternative sigma factors for appropriate downstream responses. We and others have identified orthologs of some of the Bacillus subtilis stressosome components, RsbR, RsbS, RsbT and RsbUVW in several mycobacteria and we have previously reported mutual interactions among the stressosome components RsbR, RsbS, RsbT and RsbUVW from Mycobacterium marinum. Here we provide evidence that "STAS" domains of both RsbR and RsbS are important for establishing the interaction and thus critical for stressosome assembly. Fluorescence microscopy further suggested co-localization of RsbR and RsbS in multiprotein complexes visible as co-localized fluorescent foci distributed at scattered locations in the M. marinum cytoplasm; the number, intensity and distribution of such foci changed in cells under stressed conditions. Finally, we provide bioinformatics data that 17 (of 244) mycobacteria, which lack the RsbRST genes, carry homologs of Bacillus cereus genes rsbK and rsbM indicating the existence of alternative σF activation pathways among mycobacteria.Malavika RameshRam Gopal NitharwalPhani Rama Krishna BehraB. M. Fredrik PetterssonSantanu DasguptaLeif A. KirsebomNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Malavika Ramesh Ram Gopal Nitharwal Phani Rama Krishna Behra B. M. Fredrik Pettersson Santanu Dasgupta Leif A. Kirsebom Intracellular localization of the mycobacterial stressosome complex
description	Abstract Microorganisms survive stresses by alternating the expression of genes suitable for surviving the immediate and present danger and eventually adapt to new conditions. Many bacteria have evolved a multiprotein "molecular machinery" designated the "Stressosome" that integrates different stress signals and activates alternative sigma factors for appropriate downstream responses. We and others have identified orthologs of some of the Bacillus subtilis stressosome components, RsbR, RsbS, RsbT and RsbUVW in several mycobacteria and we have previously reported mutual interactions among the stressosome components RsbR, RsbS, RsbT and RsbUVW from Mycobacterium marinum. Here we provide evidence that "STAS" domains of both RsbR and RsbS are important for establishing the interaction and thus critical for stressosome assembly. Fluorescence microscopy further suggested co-localization of RsbR and RsbS in multiprotein complexes visible as co-localized fluorescent foci distributed at scattered locations in the M. marinum cytoplasm; the number, intensity and distribution of such foci changed in cells under stressed conditions. Finally, we provide bioinformatics data that 17 (of 244) mycobacteria, which lack the RsbRST genes, carry homologs of Bacillus cereus genes rsbK and rsbM indicating the existence of alternative σF activation pathways among mycobacteria.
format	article
author	Malavika Ramesh Ram Gopal Nitharwal Phani Rama Krishna Behra B. M. Fredrik Pettersson Santanu Dasgupta Leif A. Kirsebom
author_facet	Malavika Ramesh Ram Gopal Nitharwal Phani Rama Krishna Behra B. M. Fredrik Pettersson Santanu Dasgupta Leif A. Kirsebom
author_sort	Malavika Ramesh
title	Intracellular localization of the mycobacterial stressosome complex
title_short	Intracellular localization of the mycobacterial stressosome complex
title_full	Intracellular localization of the mycobacterial stressosome complex
title_fullStr	Intracellular localization of the mycobacterial stressosome complex
title_full_unstemmed	Intracellular localization of the mycobacterial stressosome complex
title_sort	intracellular localization of the mycobacterial stressosome complex
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/539de5fd9708428cb5bd2e1d4946e45e
work_keys_str_mv	AT malavikaramesh intracellularlocalizationofthemycobacterialstressosomecomplex AT ramgopalnitharwal intracellularlocalizationofthemycobacterialstressosomecomplex AT phaniramakrishnabehra intracellularlocalizationofthemycobacterialstressosomecomplex AT bmfredrikpettersson intracellularlocalizationofthemycobacterialstressosomecomplex AT santanudasgupta intracellularlocalizationofthemycobacterialstressosomecomplex AT leifakirsebom intracellularlocalizationofthemycobacterialstressosomecomplex
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Intracellular localization of the mycobacterial stressosome complex

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