Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion.

The Toxin Complex (TC) is a large multi-subunit toxin first characterized in the insect pathogens Photorhabdus and Xenorhabdus, but now seen in a range of pathogens, including those of humans. These complexes comprise three protein subunits, A, B and C which in the Xenorhabdus toxin are found in a 4...

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Autores principales:	Guowei Yang, Carmen Sara Hernández-Rodríguez, Michael L Beeton, Paul Wilkinson, Richard H Ffrench-Constant, Nicholas R Waterfield
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/53a05a92a6d74129aee1d7c64942f0d1
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spelling	oai:doaj.org-article:53a05a92a6d74129aee1d7c64942f0d12021-11-18T06:04:26ZPdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion.1553-73661553-737410.1371/journal.ppat.1002692https://doaj.org/article/53a05a92a6d74129aee1d7c64942f0d12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22615559/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The Toxin Complex (TC) is a large multi-subunit toxin first characterized in the insect pathogens Photorhabdus and Xenorhabdus, but now seen in a range of pathogens, including those of humans. These complexes comprise three protein subunits, A, B and C which in the Xenorhabdus toxin are found in a 4:1:1 stoichiometry. Some TCs have been demonstrated to exhibit oral toxicity to insects and have the potential to be developed as a pest control technology. The lack of recognisable signal sequences in the three large component proteins hinders an understanding of their mode of secretion. Nevertheless, we have shown the Photorhabdus luminescens (Pl) Tcd complex has been shown to associate with the bacteria's surface, although some strains can also release it into the surrounding milieu. The large number of tc gene homologues in Pl make study of the export process difficult and as such we have developed and validated a heterologous Escherichia coli expression model to study the release of these important toxins. In addition to this model, we have used comparative genomics between a strain that releases high levels of Tcd into the supernatant and one that retains the toxin on its surface, to identify a protein responsible for enhancing secretion and release of these toxins. This protein is a putative lipase (Pdl1) which is regulated by a small tightly linked antagonist protein (Orf53). The identification of homologues of these in other bacteria, linked to other virulence factor operons, such as type VI secretion systems, suggests that these genes represent a general and widespread mechanism for enhancing toxin release in gram negative pathogens.Guowei YangCarmen Sara Hernández-RodríguezMichael L BeetonPaul WilkinsonRichard H Ffrench-ConstantNicholas R WaterfieldPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 5, p e1002692 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Guowei Yang Carmen Sara Hernández-Rodríguez Michael L Beeton Paul Wilkinson Richard H Ffrench-Constant Nicholas R Waterfield Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion.
description	The Toxin Complex (TC) is a large multi-subunit toxin first characterized in the insect pathogens Photorhabdus and Xenorhabdus, but now seen in a range of pathogens, including those of humans. These complexes comprise three protein subunits, A, B and C which in the Xenorhabdus toxin are found in a 4:1:1 stoichiometry. Some TCs have been demonstrated to exhibit oral toxicity to insects and have the potential to be developed as a pest control technology. The lack of recognisable signal sequences in the three large component proteins hinders an understanding of their mode of secretion. Nevertheless, we have shown the Photorhabdus luminescens (Pl) Tcd complex has been shown to associate with the bacteria's surface, although some strains can also release it into the surrounding milieu. The large number of tc gene homologues in Pl make study of the export process difficult and as such we have developed and validated a heterologous Escherichia coli expression model to study the release of these important toxins. In addition to this model, we have used comparative genomics between a strain that releases high levels of Tcd into the supernatant and one that retains the toxin on its surface, to identify a protein responsible for enhancing secretion and release of these toxins. This protein is a putative lipase (Pdl1) which is regulated by a small tightly linked antagonist protein (Orf53). The identification of homologues of these in other bacteria, linked to other virulence factor operons, such as type VI secretion systems, suggests that these genes represent a general and widespread mechanism for enhancing toxin release in gram negative pathogens.
format	article
author	Guowei Yang Carmen Sara Hernández-Rodríguez Michael L Beeton Paul Wilkinson Richard H Ffrench-Constant Nicholas R Waterfield
author_facet	Guowei Yang Carmen Sara Hernández-Rodríguez Michael L Beeton Paul Wilkinson Richard H Ffrench-Constant Nicholas R Waterfield
author_sort	Guowei Yang
title	Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion.
title_short	Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion.
title_full	Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion.
title_fullStr	Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion.
title_full_unstemmed	Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion.
title_sort	pdl1 is a putative lipase that enhances photorhabdus toxin complex secretion.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/53a05a92a6d74129aee1d7c64942f0d1
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_version_	1718424602595557376

Pdl1 is a putative lipase that enhances Photorhabdus toxin complex secretion.

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