miR-485-5p alleviates Alzheimer’s disease progression by targeting PACS1

Alzheimer’s disease (AD) is a common dementia and a heterogeneous disease. Previous research has validated that microRNAs (miRNAs) are pivotal regulators in the initiation and development of tremendous diseases including AD. MicroRNA-485-5p (miR-485-5p) was reported to be an important participant im...

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Autores principales:	He Chuan, Su Caixia, Zhang Wentong, Wan Qi
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	alzheimer’s disease pericytes mir-485-5p pacs1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/53a0e191276d4a8c964415fcbbcd5af1
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id	oai:doaj.org-article:53a0e191276d4a8c964415fcbbcd5af1
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spelling	oai:doaj.org-article:53a0e191276d4a8c964415fcbbcd5af12021-12-05T14:11:05ZmiR-485-5p alleviates Alzheimer’s disease progression by targeting PACS12081-693610.1515/tnsci-2020-0177https://doaj.org/article/53a0e191276d4a8c964415fcbbcd5af12021-09-01T00:00:00Zhttps://doi.org/10.1515/tnsci-2020-0177https://doaj.org/toc/2081-6936Alzheimer’s disease (AD) is a common dementia and a heterogeneous disease. Previous research has validated that microRNAs (miRNAs) are pivotal regulators in the initiation and development of tremendous diseases including AD. MicroRNA-485-5p (miR-485-5p) was reported to be an important participant implicated in several neurological diseases, but its role in AD still needs to be further investigated. In this research, we explored the biological function of miR-485-5p in AD. RT-qPCR revealed that miR-485-5p expression was downregulated in the hippocampus of APP/PS1 mice. Additionally, miR-485-5p overexpression facilitated the learning and memory capabilities of APP/PS1 mice according to Morris water maze test, fear conditioning test, and immunofluorescent staining. Moreover, CCK-8 assay, flow cytometric analysis, and western blot analysis suggested that miR-485-5p overexpression promoted pericyte viability and prohibited pericyte apoptosis in APP/PS1 mice. Mechanistically, miR-485-5p directly targeted PACS1 in pericytes, as shown in a luciferase reporter assay. In rescue assays, PACS1 overexpression countervailed the effect of miR-485-5p overexpression on pericyte viability and apoptosis. In conclusion, miR-485-5p ameliorates AD progression by targeting PACS1.He ChuanSu CaixiaZhang WentongWan QiDe Gruyterarticlealzheimer’s diseasepericytesmir-485-5ppacs1Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENTranslational Neuroscience, Vol 12, Iss 1, Pp 335-345 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	alzheimer’s disease pericytes mir-485-5p pacs1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	alzheimer’s disease pericytes mir-485-5p pacs1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 He Chuan Su Caixia Zhang Wentong Wan Qi miR-485-5p alleviates Alzheimer’s disease progression by targeting PACS1
description	Alzheimer’s disease (AD) is a common dementia and a heterogeneous disease. Previous research has validated that microRNAs (miRNAs) are pivotal regulators in the initiation and development of tremendous diseases including AD. MicroRNA-485-5p (miR-485-5p) was reported to be an important participant implicated in several neurological diseases, but its role in AD still needs to be further investigated. In this research, we explored the biological function of miR-485-5p in AD. RT-qPCR revealed that miR-485-5p expression was downregulated in the hippocampus of APP/PS1 mice. Additionally, miR-485-5p overexpression facilitated the learning and memory capabilities of APP/PS1 mice according to Morris water maze test, fear conditioning test, and immunofluorescent staining. Moreover, CCK-8 assay, flow cytometric analysis, and western blot analysis suggested that miR-485-5p overexpression promoted pericyte viability and prohibited pericyte apoptosis in APP/PS1 mice. Mechanistically, miR-485-5p directly targeted PACS1 in pericytes, as shown in a luciferase reporter assay. In rescue assays, PACS1 overexpression countervailed the effect of miR-485-5p overexpression on pericyte viability and apoptosis. In conclusion, miR-485-5p ameliorates AD progression by targeting PACS1.
format	article
author	He Chuan Su Caixia Zhang Wentong Wan Qi
author_facet	He Chuan Su Caixia Zhang Wentong Wan Qi
author_sort	He Chuan
title	miR-485-5p alleviates Alzheimer’s disease progression by targeting PACS1
title_short	miR-485-5p alleviates Alzheimer’s disease progression by targeting PACS1
title_full	miR-485-5p alleviates Alzheimer’s disease progression by targeting PACS1
title_fullStr	miR-485-5p alleviates Alzheimer’s disease progression by targeting PACS1
title_full_unstemmed	miR-485-5p alleviates Alzheimer’s disease progression by targeting PACS1
title_sort	mir-485-5p alleviates alzheimer’s disease progression by targeting pacs1
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/53a0e191276d4a8c964415fcbbcd5af1
work_keys_str_mv	AT hechuan mir4855palleviatesalzheimersdiseaseprogressionbytargetingpacs1 AT sucaixia mir4855palleviatesalzheimersdiseaseprogressionbytargetingpacs1 AT zhangwentong mir4855palleviatesalzheimersdiseaseprogressionbytargetingpacs1 AT wanqi mir4855palleviatesalzheimersdiseaseprogressionbytargetingpacs1
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miR-485-5p alleviates Alzheimer’s disease progression by targeting PACS1

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