miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke

Shengtao Yao,* Bo Tang,* Gang Li, Ruiming Fan, Fang Cao Department of Cerebrovascular Disease, The First Affiliated Hospital of Zunyi Medical College, Zunyi, People’s Republic of China *These authors contributed equally to this work Abstract: Ischemic stroke is one of the leading causes...

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Autores principales:	Yao ST, Tang B, Li G, Fan RM, Cao F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	miR-455 neuronal cell TRAF3 cerebral ischemic stroke Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/53a1cd4d5f63404294b97fa0fce9b5db
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spelling	oai:doaj.org-article:53a1cd4d5f63404294b97fa0fce9b5db2021-12-02T01:35:46ZmiR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke1178-2021https://doaj.org/article/53a1cd4d5f63404294b97fa0fce9b5db2016-12-01T00:00:00Zhttps://www.dovepress.com/mir-455-inhibits-neuronal-cell-death-by-targeting-traf3-in-cerebral-is-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Shengtao Yao,* Bo Tang,* Gang Li, Ruiming Fan, Fang Cao Department of Cerebrovascular Disease, The First Affiliated Hospital of Zunyi Medical College, Zunyi, People’s Republic of China *These authors contributed equally to this work Abstract: Ischemic stroke is one of the leading causes of brain disease, with high morbidity, disability, and mortality. MicroRNAs (miRNAs) have been identified as vital gene regulators in various types of human diseases. Accumulating evidence has suggested that aberrant expression of miRNAs play critical roles in the pathologies of ischemic stroke. Yet, the precise mechanism by which miRNAs control cerebral ischemic stroke remains unclear. In the present study, we explored whether miR-455 suppresses neuronal death by targeting TRAF3 in cerebral ischemic stroke. The expression levels of miR-455 and TRAF3 were detected by quantitative real-time polymerase chain reaction and Western blot. The role of miR-455 in cell death caused by oxygen–glucose deprivation (OGD) was assessed using Cell Counting Kit-8 (CCK-8) assay. The influence of miR-455 on infarct volume was evaluated in mouse brain after middle cerebral artery occlusion (MCAO). Bioinformatics softwares and luciferase analysis were used to find and confirm the targets of miR-455. The results showed that the expression levels of miR-455 significantly decreased in primary neuronal cells subjected to OGD and mouse brain subjected to MCAO. In addition, forced expression of miR-455 inhibited neuronal death and weakened ischemic brain infarction in focal ischemia-stroked mice. Furthermore, TRAF3 was proved to be a direct target of miR-455, and miR-455 could negatively suppress TRAF3 expression. Biological function analysis showed that TRAF3 silencing displayed the neuroprotective effect in ischemic stroke and could enhance miR-455-induced positive impact on ischemic injury both in vitro and in vivo. Taken together, miR-455 played a vital role in protecting neuronal cells from death by downregulating TRAF3 protein expression. These findings may represent a novel latent therapeutic target for cerebral ischemic stroke. Keywords: miR-455, neuronal cell, TRAF3, cerebral ischemic stroke, MCAOYao STTang BLi GFan RMCao FDove Medical PressarticlemiR-455neuronal cellTRAF3cerebral ischemic strokeNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 12, Pp 3083-3092 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	miR-455 neuronal cell TRAF3 cerebral ischemic stroke Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	miR-455 neuronal cell TRAF3 cerebral ischemic stroke Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Yao ST Tang B Li G Fan RM Cao F miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke
description	Shengtao Yao,* Bo Tang,* Gang Li, Ruiming Fan, Fang Cao Department of Cerebrovascular Disease, The First Affiliated Hospital of Zunyi Medical College, Zunyi, People’s Republic of China *These authors contributed equally to this work Abstract: Ischemic stroke is one of the leading causes of brain disease, with high morbidity, disability, and mortality. MicroRNAs (miRNAs) have been identified as vital gene regulators in various types of human diseases. Accumulating evidence has suggested that aberrant expression of miRNAs play critical roles in the pathologies of ischemic stroke. Yet, the precise mechanism by which miRNAs control cerebral ischemic stroke remains unclear. In the present study, we explored whether miR-455 suppresses neuronal death by targeting TRAF3 in cerebral ischemic stroke. The expression levels of miR-455 and TRAF3 were detected by quantitative real-time polymerase chain reaction and Western blot. The role of miR-455 in cell death caused by oxygen–glucose deprivation (OGD) was assessed using Cell Counting Kit-8 (CCK-8) assay. The influence of miR-455 on infarct volume was evaluated in mouse brain after middle cerebral artery occlusion (MCAO). Bioinformatics softwares and luciferase analysis were used to find and confirm the targets of miR-455. The results showed that the expression levels of miR-455 significantly decreased in primary neuronal cells subjected to OGD and mouse brain subjected to MCAO. In addition, forced expression of miR-455 inhibited neuronal death and weakened ischemic brain infarction in focal ischemia-stroked mice. Furthermore, TRAF3 was proved to be a direct target of miR-455, and miR-455 could negatively suppress TRAF3 expression. Biological function analysis showed that TRAF3 silencing displayed the neuroprotective effect in ischemic stroke and could enhance miR-455-induced positive impact on ischemic injury both in vitro and in vivo. Taken together, miR-455 played a vital role in protecting neuronal cells from death by downregulating TRAF3 protein expression. These findings may represent a novel latent therapeutic target for cerebral ischemic stroke. Keywords: miR-455, neuronal cell, TRAF3, cerebral ischemic stroke, MCAO
format	article
author	Yao ST Tang B Li G Fan RM Cao F
author_facet	Yao ST Tang B Li G Fan RM Cao F
author_sort	Yao ST
title	miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke
title_short	miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke
title_full	miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke
title_fullStr	miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke
title_full_unstemmed	miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke
title_sort	mir-455 inhibits neuronal cell death by targeting traf3 in cerebral ischemic stroke
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/53a1cd4d5f63404294b97fa0fce9b5db
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miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke

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