Estrogen-related receptor alpha modulates lactate dehydrogenase activity in thyroid tumors.

Metabolic modifications of tumor cells are hallmarks of cancer. They exhibit an altered metabolism that allows them to sustain higher proliferation rates in hostile environment outside the cell. In thyroid tumors, the expression of the estrogen-related receptor α (ERRα), a major factor of metabolic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Delphine Mirebeau-Prunier, Soazig Le Pennec, Caroline Jacques, Jean-Fred Fontaine, Naig Gueguen, Nathalie Boutet-Bouzamondo, Audrey Donnart, Yves Malthièry, Frédérique Savagner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/53a2b26cfc1a405c842e10c235e3de98
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:53a2b26cfc1a405c842e10c235e3de98
record_format	dspace
spelling	oai:doaj.org-article:53a2b26cfc1a405c842e10c235e3de982021-11-18T07:53:35ZEstrogen-related receptor alpha modulates lactate dehydrogenase activity in thyroid tumors.1932-620310.1371/journal.pone.0058683https://doaj.org/article/53a2b26cfc1a405c842e10c235e3de982013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23516535/?tool=EBIhttps://doaj.org/toc/1932-6203Metabolic modifications of tumor cells are hallmarks of cancer. They exhibit an altered metabolism that allows them to sustain higher proliferation rates in hostile environment outside the cell. In thyroid tumors, the expression of the estrogen-related receptor α (ERRα), a major factor of metabolic adaptation, is closely related to the oxidative metabolism and the proliferative status of the cells. To elucidate the role played by ERRα in the glycolytic adaptation of tumor cells, we focused on the regulation of lactate dehydrogenases A and B (LDHA, LDHB) and the LDHA/LDHB ratio. Our study included tissue samples from 10 classical and 10 oncocytic variants of follicular thyroid tumors and 10 normal thyroid tissues, as well as samples from three human thyroid tumor cell lines: FTC-133, XTC.UC1 and RO82W-1. We identified multiple cis-acting promoter elements for ERRα, in both the LDHA and LDHB genes. The interaction between ERRα and LDH promoters was confirmed by chromatin immunoprecipitation assays and in vitro analysis for LDHB. Using knock-in and knock-out cellular models, we found an inverse correlation between ERRα expression and LDH activity. This suggests that thyroid tumor cells may reprogram their metabolic pathways through the up-regulation of ERRα by a process distinct from that proposed by the recently revisited Warburg hypothesis.Delphine Mirebeau-PrunierSoazig Le PennecCaroline JacquesJean-Fred FontaineNaig GueguenNathalie Boutet-BouzamondoAudrey DonnartYves MalthièryFrédérique SavagnerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58683 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Delphine Mirebeau-Prunier Soazig Le Pennec Caroline Jacques Jean-Fred Fontaine Naig Gueguen Nathalie Boutet-Bouzamondo Audrey Donnart Yves Malthièry Frédérique Savagner Estrogen-related receptor alpha modulates lactate dehydrogenase activity in thyroid tumors.
description	Metabolic modifications of tumor cells are hallmarks of cancer. They exhibit an altered metabolism that allows them to sustain higher proliferation rates in hostile environment outside the cell. In thyroid tumors, the expression of the estrogen-related receptor α (ERRα), a major factor of metabolic adaptation, is closely related to the oxidative metabolism and the proliferative status of the cells. To elucidate the role played by ERRα in the glycolytic adaptation of tumor cells, we focused on the regulation of lactate dehydrogenases A and B (LDHA, LDHB) and the LDHA/LDHB ratio. Our study included tissue samples from 10 classical and 10 oncocytic variants of follicular thyroid tumors and 10 normal thyroid tissues, as well as samples from three human thyroid tumor cell lines: FTC-133, XTC.UC1 and RO82W-1. We identified multiple cis-acting promoter elements for ERRα, in both the LDHA and LDHB genes. The interaction between ERRα and LDH promoters was confirmed by chromatin immunoprecipitation assays and in vitro analysis for LDHB. Using knock-in and knock-out cellular models, we found an inverse correlation between ERRα expression and LDH activity. This suggests that thyroid tumor cells may reprogram their metabolic pathways through the up-regulation of ERRα by a process distinct from that proposed by the recently revisited Warburg hypothesis.
format	article
author	Delphine Mirebeau-Prunier Soazig Le Pennec Caroline Jacques Jean-Fred Fontaine Naig Gueguen Nathalie Boutet-Bouzamondo Audrey Donnart Yves Malthièry Frédérique Savagner
author_facet	Delphine Mirebeau-Prunier Soazig Le Pennec Caroline Jacques Jean-Fred Fontaine Naig Gueguen Nathalie Boutet-Bouzamondo Audrey Donnart Yves Malthièry Frédérique Savagner
author_sort	Delphine Mirebeau-Prunier
title	Estrogen-related receptor alpha modulates lactate dehydrogenase activity in thyroid tumors.
title_short	Estrogen-related receptor alpha modulates lactate dehydrogenase activity in thyroid tumors.
title_full	Estrogen-related receptor alpha modulates lactate dehydrogenase activity in thyroid tumors.
title_fullStr	Estrogen-related receptor alpha modulates lactate dehydrogenase activity in thyroid tumors.
title_full_unstemmed	Estrogen-related receptor alpha modulates lactate dehydrogenase activity in thyroid tumors.
title_sort	estrogen-related receptor alpha modulates lactate dehydrogenase activity in thyroid tumors.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/53a2b26cfc1a405c842e10c235e3de98
work_keys_str_mv	AT delphinemirebeauprunier estrogenrelatedreceptoralphamodulateslactatedehydrogenaseactivityinthyroidtumors AT soaziglepennec estrogenrelatedreceptoralphamodulateslactatedehydrogenaseactivityinthyroidtumors AT carolinejacques estrogenrelatedreceptoralphamodulateslactatedehydrogenaseactivityinthyroidtumors AT jeanfredfontaine estrogenrelatedreceptoralphamodulateslactatedehydrogenaseactivityinthyroidtumors AT naiggueguen estrogenrelatedreceptoralphamodulateslactatedehydrogenaseactivityinthyroidtumors AT nathalieboutetbouzamondo estrogenrelatedreceptoralphamodulateslactatedehydrogenaseactivityinthyroidtumors AT audreydonnart estrogenrelatedreceptoralphamodulateslactatedehydrogenaseactivityinthyroidtumors AT yvesmalthiery estrogenrelatedreceptoralphamodulateslactatedehydrogenaseactivityinthyroidtumors AT frederiquesavagner estrogenrelatedreceptoralphamodulateslactatedehydrogenaseactivityinthyroidtumors
_version_	1718422812955246592

Estrogen-related receptor alpha modulates lactate dehydrogenase activity in thyroid tumors.

Ejemplares similares