Effect of dietary zinc oxide on morphological characteristics, mucin composition and gene expression in the colon of weaned piglets.

The trace element zinc is often used in the diet of weaned piglets, as high doses have resulted in positive effects on intestinal health. However, the majority of previous studies evaluated zinc supplementations for a short period only and focused on the small intestine. The hypothesis of the presen...

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Autores principales:	Ping Liu, Robert Pieper, Juliane Rieger, Wilfried Vahjen, Roger Davin, Johanna Plendl, Wilfried Meyer, Jürgen Zentek
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/53ac16a1db2b4c7dbac431a9ff290e5c
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spelling	oai:doaj.org-article:53ac16a1db2b4c7dbac431a9ff290e5c2021-11-18T08:29:06ZEffect of dietary zinc oxide on morphological characteristics, mucin composition and gene expression in the colon of weaned piglets.1932-620310.1371/journal.pone.0091091https://doaj.org/article/53ac16a1db2b4c7dbac431a9ff290e5c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24609095/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The trace element zinc is often used in the diet of weaned piglets, as high doses have resulted in positive effects on intestinal health. However, the majority of previous studies evaluated zinc supplementations for a short period only and focused on the small intestine. The hypothesis of the present study was that low, medium and high levels of dietary zinc (57, 164 and 2,425 mg Zn/kg from zinc oxide) would affect colonic morphology and innate host defense mechanisms across 4 weeks post-weaning. Histological examinations were conducted regarding the colonic morphology and neutral, acidic, sialylated and sulphated mucins. The mRNA expression levels of mucin (MUC) 1, 2, 13, 20, toll-like receptor (TLR) 2, 4, interleukin (IL)-1β, 8, 10, interferon-γ (IFN-γ) and transforming growth factor-β (TGF-β) were also measured. The colonic crypt area increased in an age-depending manner, and the greatest area was found with medium concentration of dietary zinc. With the high concentration of dietary zinc, the number of goblet cells containing mixed neutral-acidic mucins and total mucins increased. Sialomucin containing goblet cells increased age-dependently. The expression of MUC2 increased with age and reached the highest level at 47 days of age. The expression levels of TLR2 and 4 decreased with age. The mRNA expression of TLR4 and the pro-inflammatory cytokine IL-8 were down-regulated with high dietary zinc treatment, while piglets fed with medium dietary zinc had the highest expression. It is concluded that dietary zinc level had a clear impact on colonic morphology, mucin profiles and immunological traits in piglets after weaning. Those changes might support local defense mechanisms and affect colonic physiology and contribute to the reported reduction of post-weaning diarrhea.Ping LiuRobert PieperJuliane RiegerWilfried VahjenRoger DavinJohanna PlendlWilfried MeyerJürgen ZentekPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91091 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ping Liu Robert Pieper Juliane Rieger Wilfried Vahjen Roger Davin Johanna Plendl Wilfried Meyer Jürgen Zentek Effect of dietary zinc oxide on morphological characteristics, mucin composition and gene expression in the colon of weaned piglets.
description	The trace element zinc is often used in the diet of weaned piglets, as high doses have resulted in positive effects on intestinal health. However, the majority of previous studies evaluated zinc supplementations for a short period only and focused on the small intestine. The hypothesis of the present study was that low, medium and high levels of dietary zinc (57, 164 and 2,425 mg Zn/kg from zinc oxide) would affect colonic morphology and innate host defense mechanisms across 4 weeks post-weaning. Histological examinations were conducted regarding the colonic morphology and neutral, acidic, sialylated and sulphated mucins. The mRNA expression levels of mucin (MUC) 1, 2, 13, 20, toll-like receptor (TLR) 2, 4, interleukin (IL)-1β, 8, 10, interferon-γ (IFN-γ) and transforming growth factor-β (TGF-β) were also measured. The colonic crypt area increased in an age-depending manner, and the greatest area was found with medium concentration of dietary zinc. With the high concentration of dietary zinc, the number of goblet cells containing mixed neutral-acidic mucins and total mucins increased. Sialomucin containing goblet cells increased age-dependently. The expression of MUC2 increased with age and reached the highest level at 47 days of age. The expression levels of TLR2 and 4 decreased with age. The mRNA expression of TLR4 and the pro-inflammatory cytokine IL-8 were down-regulated with high dietary zinc treatment, while piglets fed with medium dietary zinc had the highest expression. It is concluded that dietary zinc level had a clear impact on colonic morphology, mucin profiles and immunological traits in piglets after weaning. Those changes might support local defense mechanisms and affect colonic physiology and contribute to the reported reduction of post-weaning diarrhea.
format	article
author	Ping Liu Robert Pieper Juliane Rieger Wilfried Vahjen Roger Davin Johanna Plendl Wilfried Meyer Jürgen Zentek
author_facet	Ping Liu Robert Pieper Juliane Rieger Wilfried Vahjen Roger Davin Johanna Plendl Wilfried Meyer Jürgen Zentek
author_sort	Ping Liu
title	Effect of dietary zinc oxide on morphological characteristics, mucin composition and gene expression in the colon of weaned piglets.
title_short	Effect of dietary zinc oxide on morphological characteristics, mucin composition and gene expression in the colon of weaned piglets.
title_full	Effect of dietary zinc oxide on morphological characteristics, mucin composition and gene expression in the colon of weaned piglets.
title_fullStr	Effect of dietary zinc oxide on morphological characteristics, mucin composition and gene expression in the colon of weaned piglets.
title_full_unstemmed	Effect of dietary zinc oxide on morphological characteristics, mucin composition and gene expression in the colon of weaned piglets.
title_sort	effect of dietary zinc oxide on morphological characteristics, mucin composition and gene expression in the colon of weaned piglets.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/53ac16a1db2b4c7dbac431a9ff290e5c
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Effect of dietary zinc oxide on morphological characteristics, mucin composition and gene expression in the colon of weaned piglets.

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