Contribution of plasma membrane lipid domains to red blood cell (re)shaping

Abstract Although lipid domains have been evidenced in several living cell plasma membranes, their roles remain largely unclear. We here investigated whether they could contribute to function-associated cell (re)shaping. To address this question, we used erythrocytes as cellular model since they (i)...

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Autores principales:	C. Leonard, L. Conrard, M. Guthmann, H. Pollet, M. Carquin, C. Vermylen, P. Gailly, P. Van Der Smissen, M. P. Mingeot-Leclercq, D. Tyteca
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/53b3e96f60d34bdbb6a7c77e0de021b1
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spelling	oai:doaj.org-article:53b3e96f60d34bdbb6a7c77e0de021b12021-12-02T11:53:07ZContribution of plasma membrane lipid domains to red blood cell (re)shaping10.1038/s41598-017-04388-z2045-2322https://doaj.org/article/53b3e96f60d34bdbb6a7c77e0de021b12017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04388-zhttps://doaj.org/toc/2045-2322Abstract Although lipid domains have been evidenced in several living cell plasma membranes, their roles remain largely unclear. We here investigated whether they could contribute to function-associated cell (re)shaping. To address this question, we used erythrocytes as cellular model since they (i) exhibit a specific biconcave shape, allowing for reversible deformation in blood circulation, which is lost by membrane vesiculation upon aging; and (ii) display at their outer plasma membrane leaflet two types of submicrometric domains differently enriched in cholesterol and sphingomyelin. We here reveal the specific association of cholesterol- and sphingomyelin-enriched domains with distinct curvature areas of the erythrocyte biconcave membrane. Upon erythrocyte deformation, cholesterol-enriched domains gathered in high curvature areas. In contrast, sphingomyelin-enriched domains increased in abundance upon calcium efflux during shape restoration. Upon erythrocyte storage at 4 °C (to mimick aging), lipid domains appeared as specific vesiculation sites. Altogether, our data indicate that lipid domains could contribute to erythrocyte function-associated (re)shaping.C. LeonardL. ConrardM. GuthmannH. PolletM. CarquinC. VermylenP. GaillyP. Van Der SmissenM. P. Mingeot-LeclercqD. TytecaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q C. Leonard L. Conrard M. Guthmann H. Pollet M. Carquin C. Vermylen P. Gailly P. Van Der Smissen M. P. Mingeot-Leclercq D. Tyteca Contribution of plasma membrane lipid domains to red blood cell (re)shaping
description	Abstract Although lipid domains have been evidenced in several living cell plasma membranes, their roles remain largely unclear. We here investigated whether they could contribute to function-associated cell (re)shaping. To address this question, we used erythrocytes as cellular model since they (i) exhibit a specific biconcave shape, allowing for reversible deformation in blood circulation, which is lost by membrane vesiculation upon aging; and (ii) display at their outer plasma membrane leaflet two types of submicrometric domains differently enriched in cholesterol and sphingomyelin. We here reveal the specific association of cholesterol- and sphingomyelin-enriched domains with distinct curvature areas of the erythrocyte biconcave membrane. Upon erythrocyte deformation, cholesterol-enriched domains gathered in high curvature areas. In contrast, sphingomyelin-enriched domains increased in abundance upon calcium efflux during shape restoration. Upon erythrocyte storage at 4 °C (to mimick aging), lipid domains appeared as specific vesiculation sites. Altogether, our data indicate that lipid domains could contribute to erythrocyte function-associated (re)shaping.
format	article
author	C. Leonard L. Conrard M. Guthmann H. Pollet M. Carquin C. Vermylen P. Gailly P. Van Der Smissen M. P. Mingeot-Leclercq D. Tyteca
author_facet	C. Leonard L. Conrard M. Guthmann H. Pollet M. Carquin C. Vermylen P. Gailly P. Van Der Smissen M. P. Mingeot-Leclercq D. Tyteca
author_sort	C. Leonard
title	Contribution of plasma membrane lipid domains to red blood cell (re)shaping
title_short	Contribution of plasma membrane lipid domains to red blood cell (re)shaping
title_full	Contribution of plasma membrane lipid domains to red blood cell (re)shaping
title_fullStr	Contribution of plasma membrane lipid domains to red blood cell (re)shaping
title_full_unstemmed	Contribution of plasma membrane lipid domains to red blood cell (re)shaping
title_sort	contribution of plasma membrane lipid domains to red blood cell (re)shaping
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/53b3e96f60d34bdbb6a7c77e0de021b1
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Contribution of plasma membrane lipid domains to red blood cell (re)shaping

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