Immunological Microenvironment Alterations in Follicles of Patients With Autoimmune Thyroiditis

Autoimmune thyroiditis (AIT) is the most prevalent autoimmune endocrine disease, with a higher incidence in women than in men. Immunological abnormalities may lead to the impairment of ovarian folliculogenesis; however, whether the presence of AIT affects immunological microenvironment in follicles...

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Autores principales:	Ning Huang, Dandan Liu, Ying Lian, Hongbin Chi, Jie Qiao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	autoimmune thyroiditis ovarian microenvironment follicle development IFNγ-CXCL9/10/11-CXCR3+ T lymphocyte follicular fluid Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/53b5760c6f29478e9b4f61df74b9a0d6
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spelling	oai:doaj.org-article:53b5760c6f29478e9b4f61df74b9a0d62021-11-17T04:52:07ZImmunological Microenvironment Alterations in Follicles of Patients With Autoimmune Thyroiditis1664-322410.3389/fimmu.2021.770852https://doaj.org/article/53b5760c6f29478e9b4f61df74b9a0d62021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.770852/fullhttps://doaj.org/toc/1664-3224Autoimmune thyroiditis (AIT) is the most prevalent autoimmune endocrine disease, with a higher incidence in women than in men. Immunological abnormalities may lead to the impairment of ovarian folliculogenesis; however, whether the presence of AIT affects immunological microenvironment in follicles remains controversial. We performed a cross-sectional study including 122 patients, aged 20–40 years, who underwent IVF/ICSI treatment owing to isolated male or tube factor infertility. Patients were divided into AIT and control groups according to clinical presentation, thyroid function, and thyroid autoantibody measurements. Follicular fluid was collected and the distribution of cytokines/chemokines in follicular fluid was measured by flow cytometry using multiplex bead assays between the two groups. Based on differences in levels of intrafollicular chemokines and cytokines between the AIT and control groups, the relevant inflammatory cascade was further demonstrated. Among the 12 chemokines analyzed, three (CXCL9, CXCL10, and CXCL11) showed significantly elevated levels in the follicular fluid of patients with AIT. Among the 11 cytokines detected, compared with those in the control group, significantly higher levels of IFNγ were observed in patients with AIT. IFNγ dose-dependently stimulated the expression and secretion of CXCL9/10/11 in cultured primary granulosa cells. The percentage of CXCR3+ T lymphocytes was significantly elevated in the follicular microenvironment of patients with AIT. We concluded that the IFNγ-CXCL9/10/11-CXCR3+ T lymphocyte inflammatory cascade is activated in the follicular microenvironment of patients with AIT. These findings indicate that a considerable immune imbalance occurred in the follicular microenvironment of patients with AIT.Ning HuangNing HuangNing HuangNing HuangDandan LiuDandan LiuDandan LiuDandan LiuYing LianYing LianYing LianYing LianHongbin ChiHongbin ChiHongbin ChiHongbin ChiJie QiaoJie QiaoJie QiaoJie QiaoFrontiers Media S.A.articleautoimmune thyroiditisovarian microenvironmentfollicle developmentIFNγ-CXCL9/10/11-CXCR3+ T lymphocytefollicular fluidImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	autoimmune thyroiditis ovarian microenvironment follicle development IFNγ-CXCL9/10/11-CXCR3+ T lymphocyte follicular fluid Immunologic diseases. Allergy RC581-607
spellingShingle	autoimmune thyroiditis ovarian microenvironment follicle development IFNγ-CXCL9/10/11-CXCR3+ T lymphocyte follicular fluid Immunologic diseases. Allergy RC581-607 Ning Huang Ning Huang Ning Huang Ning Huang Dandan Liu Dandan Liu Dandan Liu Dandan Liu Ying Lian Ying Lian Ying Lian Ying Lian Hongbin Chi Hongbin Chi Hongbin Chi Hongbin Chi Jie Qiao Jie Qiao Jie Qiao Jie Qiao Immunological Microenvironment Alterations in Follicles of Patients With Autoimmune Thyroiditis
description	Autoimmune thyroiditis (AIT) is the most prevalent autoimmune endocrine disease, with a higher incidence in women than in men. Immunological abnormalities may lead to the impairment of ovarian folliculogenesis; however, whether the presence of AIT affects immunological microenvironment in follicles remains controversial. We performed a cross-sectional study including 122 patients, aged 20–40 years, who underwent IVF/ICSI treatment owing to isolated male or tube factor infertility. Patients were divided into AIT and control groups according to clinical presentation, thyroid function, and thyroid autoantibody measurements. Follicular fluid was collected and the distribution of cytokines/chemokines in follicular fluid was measured by flow cytometry using multiplex bead assays between the two groups. Based on differences in levels of intrafollicular chemokines and cytokines between the AIT and control groups, the relevant inflammatory cascade was further demonstrated. Among the 12 chemokines analyzed, three (CXCL9, CXCL10, and CXCL11) showed significantly elevated levels in the follicular fluid of patients with AIT. Among the 11 cytokines detected, compared with those in the control group, significantly higher levels of IFNγ were observed in patients with AIT. IFNγ dose-dependently stimulated the expression and secretion of CXCL9/10/11 in cultured primary granulosa cells. The percentage of CXCR3+ T lymphocytes was significantly elevated in the follicular microenvironment of patients with AIT. We concluded that the IFNγ-CXCL9/10/11-CXCR3+ T lymphocyte inflammatory cascade is activated in the follicular microenvironment of patients with AIT. These findings indicate that a considerable immune imbalance occurred in the follicular microenvironment of patients with AIT.
format	article
author	Ning Huang Ning Huang Ning Huang Ning Huang Dandan Liu Dandan Liu Dandan Liu Dandan Liu Ying Lian Ying Lian Ying Lian Ying Lian Hongbin Chi Hongbin Chi Hongbin Chi Hongbin Chi Jie Qiao Jie Qiao Jie Qiao Jie Qiao
author_facet	Ning Huang Ning Huang Ning Huang Ning Huang Dandan Liu Dandan Liu Dandan Liu Dandan Liu Ying Lian Ying Lian Ying Lian Ying Lian Hongbin Chi Hongbin Chi Hongbin Chi Hongbin Chi Jie Qiao Jie Qiao Jie Qiao Jie Qiao
author_sort	Ning Huang
title	Immunological Microenvironment Alterations in Follicles of Patients With Autoimmune Thyroiditis
title_short	Immunological Microenvironment Alterations in Follicles of Patients With Autoimmune Thyroiditis
title_full	Immunological Microenvironment Alterations in Follicles of Patients With Autoimmune Thyroiditis
title_fullStr	Immunological Microenvironment Alterations in Follicles of Patients With Autoimmune Thyroiditis
title_full_unstemmed	Immunological Microenvironment Alterations in Follicles of Patients With Autoimmune Thyroiditis
title_sort	immunological microenvironment alterations in follicles of patients with autoimmune thyroiditis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/53b5760c6f29478e9b4f61df74b9a0d6
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Immunological Microenvironment Alterations in Follicles of Patients With Autoimmune Thyroiditis

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