Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation

Abstract Escherichia coli is the most common bacterium isolated from urine and stone matrix of calcium oxalate (CaOx) stone formers. Whether it has pathogenic role(s) in kidney stone formation or is only entrapped inside the stone remains unclear. We thus evaluated differences between E. coli isolat...

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Autores principales:	Piyawan Amimanan, Ratree Tavichakorntrakool, Kedsarin Fong-ngern, Pipat Sribenjalux, Aroonlug Lulitanond, Vitoon Prasongwatana, Chaisiri Wongkham, Patcharee Boonsiri, Jariya Umka Welbat, Visith Thongboonkerd
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/53bfdd37746546288f27c5d091ecbff9
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spelling	oai:doaj.org-article:53bfdd37746546288f27c5d091ecbff92021-12-02T16:08:09ZElongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation10.1038/s41598-017-03213-x2045-2322https://doaj.org/article/53bfdd37746546288f27c5d091ecbff92017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03213-xhttps://doaj.org/toc/2045-2322Abstract Escherichia coli is the most common bacterium isolated from urine and stone matrix of calcium oxalate (CaOx) stone formers. Whether it has pathogenic role(s) in kidney stone formation or is only entrapped inside the stone remains unclear. We thus evaluated differences between E. coli isolated from urine of patients with kidney stone (EUK) and that from patients with urinary tract infection (UTI) without stone (EUU). From 100 stone formers and 200 UTI patients, only four pairs of EUK/EUU isolates had identical antimicrobial susceptibility patterns. Proteomic analysis revealed nine common differentially expressed proteins. Among these, the greater level of elongation factor Tu (EF-Tu) in EUK was validated by Western blotting. Outer membrane vesicles (OMVs) derived from EUK had greater promoting activities on CaOx crystallization, crystal growth and aggregation as compared to those derived from EUU. Neutralizing the OMVs of EUK with monoclonal anti-EF-Tu antibody, not with an isotype antibody, significantly reduced all these OMVs-induced promoting effects. Moreover, immunofluorescence staining of EF-Tu on bacterial cell surface confirmed the greater expression of surface EF-Tu on EUK (vs. EUU). Our data indicate that surface EF-Tu and OMVs play significant roles in promoting activities of E. coli on CaOx crystallization, crystal growth and aggregation.Piyawan AmimananRatree TavichakorntrakoolKedsarin Fong-ngernPipat SribenjaluxAroonlug LulitanondVitoon PrasongwatanaChaisiri WongkhamPatcharee BoonsiriJariya Umka WelbatVisith ThongboonkerdNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Piyawan Amimanan Ratree Tavichakorntrakool Kedsarin Fong-ngern Pipat Sribenjalux Aroonlug Lulitanond Vitoon Prasongwatana Chaisiri Wongkham Patcharee Boonsiri Jariya Umka Welbat Visith Thongboonkerd Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation
description	Abstract Escherichia coli is the most common bacterium isolated from urine and stone matrix of calcium oxalate (CaOx) stone formers. Whether it has pathogenic role(s) in kidney stone formation or is only entrapped inside the stone remains unclear. We thus evaluated differences between E. coli isolated from urine of patients with kidney stone (EUK) and that from patients with urinary tract infection (UTI) without stone (EUU). From 100 stone formers and 200 UTI patients, only four pairs of EUK/EUU isolates had identical antimicrobial susceptibility patterns. Proteomic analysis revealed nine common differentially expressed proteins. Among these, the greater level of elongation factor Tu (EF-Tu) in EUK was validated by Western blotting. Outer membrane vesicles (OMVs) derived from EUK had greater promoting activities on CaOx crystallization, crystal growth and aggregation as compared to those derived from EUU. Neutralizing the OMVs of EUK with monoclonal anti-EF-Tu antibody, not with an isotype antibody, significantly reduced all these OMVs-induced promoting effects. Moreover, immunofluorescence staining of EF-Tu on bacterial cell surface confirmed the greater expression of surface EF-Tu on EUK (vs. EUU). Our data indicate that surface EF-Tu and OMVs play significant roles in promoting activities of E. coli on CaOx crystallization, crystal growth and aggregation.
format	article
author	Piyawan Amimanan Ratree Tavichakorntrakool Kedsarin Fong-ngern Pipat Sribenjalux Aroonlug Lulitanond Vitoon Prasongwatana Chaisiri Wongkham Patcharee Boonsiri Jariya Umka Welbat Visith Thongboonkerd
author_facet	Piyawan Amimanan Ratree Tavichakorntrakool Kedsarin Fong-ngern Pipat Sribenjalux Aroonlug Lulitanond Vitoon Prasongwatana Chaisiri Wongkham Patcharee Boonsiri Jariya Umka Welbat Visith Thongboonkerd
author_sort	Piyawan Amimanan
title	Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation
title_short	Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation
title_full	Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation
title_fullStr	Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation
title_full_unstemmed	Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation
title_sort	elongation factor tu on escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/53bfdd37746546288f27c5d091ecbff9
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_version_	1718384594639650816

Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation

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