Soil gas probes for monitoring trace gas messengers of microbial activity

Abstract Soil microbes vigorously produce and consume gases that reflect active soil biogeochemical processes. Soil gas measurements are therefore a powerful tool to monitor microbial activity. Yet, the majority of soil gases lack non-disruptive subsurface measurement methods at spatiotemporal scale...

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Autores principales:	Joseph R. Roscioli, Laura K. Meredith, Joanne H. Shorter, Juliana Gil-Loaiza, Till H. M. Volkmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/53c45895725041e187afc2e18d7e8101
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spelling	oai:doaj.org-article:53c45895725041e187afc2e18d7e81012021-12-02T14:26:16ZSoil gas probes for monitoring trace gas messengers of microbial activity10.1038/s41598-021-86930-82045-2322https://doaj.org/article/53c45895725041e187afc2e18d7e81012021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86930-8https://doaj.org/toc/2045-2322Abstract Soil microbes vigorously produce and consume gases that reflect active soil biogeochemical processes. Soil gas measurements are therefore a powerful tool to monitor microbial activity. Yet, the majority of soil gases lack non-disruptive subsurface measurement methods at spatiotemporal scales relevant to microbial processes and soil structure. To address this need, we developed a soil gas sampling system that uses novel diffusive soil probes and sample transfer approaches for high-resolution sampling from discrete subsurface regions. Probe sampling requires transferring soil gas samples to above-ground gas analyzers where concentrations and isotopologues are measured. Obtaining representative soil gas samples has historically required balancing disruption to soil gas composition with measurement frequency and analyzer volume demand. These considerations have limited attempts to quantify trace gas spatial concentration gradients and heterogeneity at scales relevant to the soil microbiome. Here, we describe our new flexible diffusive probe sampling system integrated with a modified, reduced volume trace gas analyzer and demonstrate its application for subsurface monitoring of biogeochemical cycling of nitrous oxide (N2O) and its site-specific isotopologues, methane, carbon dioxide, and nitric oxide in controlled soil columns. The sampling system observed reproducible responses of soil gas concentrations to manipulations of soil nutrients and redox state, providing a new window into the microbial response to these key environmental forcings. Using site-specific N2O isotopologues as indicators of microbial processes, we constrain the dynamics of in situ microbial activity. Unlocking trace gas messengers of microbial activity will complement -omics approaches, challenge subsurface models, and improve understanding of soil heterogeneity to disentangle interactive processes in the subsurface biome.Joseph R. RoscioliLaura K. MeredithJoanne H. ShorterJuliana Gil-LoaizaTill H. M. VolkmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joseph R. Roscioli Laura K. Meredith Joanne H. Shorter Juliana Gil-Loaiza Till H. M. Volkmann Soil gas probes for monitoring trace gas messengers of microbial activity
description	Abstract Soil microbes vigorously produce and consume gases that reflect active soil biogeochemical processes. Soil gas measurements are therefore a powerful tool to monitor microbial activity. Yet, the majority of soil gases lack non-disruptive subsurface measurement methods at spatiotemporal scales relevant to microbial processes and soil structure. To address this need, we developed a soil gas sampling system that uses novel diffusive soil probes and sample transfer approaches for high-resolution sampling from discrete subsurface regions. Probe sampling requires transferring soil gas samples to above-ground gas analyzers where concentrations and isotopologues are measured. Obtaining representative soil gas samples has historically required balancing disruption to soil gas composition with measurement frequency and analyzer volume demand. These considerations have limited attempts to quantify trace gas spatial concentration gradients and heterogeneity at scales relevant to the soil microbiome. Here, we describe our new flexible diffusive probe sampling system integrated with a modified, reduced volume trace gas analyzer and demonstrate its application for subsurface monitoring of biogeochemical cycling of nitrous oxide (N2O) and its site-specific isotopologues, methane, carbon dioxide, and nitric oxide in controlled soil columns. The sampling system observed reproducible responses of soil gas concentrations to manipulations of soil nutrients and redox state, providing a new window into the microbial response to these key environmental forcings. Using site-specific N2O isotopologues as indicators of microbial processes, we constrain the dynamics of in situ microbial activity. Unlocking trace gas messengers of microbial activity will complement -omics approaches, challenge subsurface models, and improve understanding of soil heterogeneity to disentangle interactive processes in the subsurface biome.
format	article
author	Joseph R. Roscioli Laura K. Meredith Joanne H. Shorter Juliana Gil-Loaiza Till H. M. Volkmann
author_facet	Joseph R. Roscioli Laura K. Meredith Joanne H. Shorter Juliana Gil-Loaiza Till H. M. Volkmann
author_sort	Joseph R. Roscioli
title	Soil gas probes for monitoring trace gas messengers of microbial activity
title_short	Soil gas probes for monitoring trace gas messengers of microbial activity
title_full	Soil gas probes for monitoring trace gas messengers of microbial activity
title_fullStr	Soil gas probes for monitoring trace gas messengers of microbial activity
title_full_unstemmed	Soil gas probes for monitoring trace gas messengers of microbial activity
title_sort	soil gas probes for monitoring trace gas messengers of microbial activity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/53c45895725041e187afc2e18d7e8101
work_keys_str_mv	AT josephrroscioli soilgasprobesformonitoringtracegasmessengersofmicrobialactivity AT laurakmeredith soilgasprobesformonitoringtracegasmessengersofmicrobialactivity AT joannehshorter soilgasprobesformonitoringtracegasmessengersofmicrobialactivity AT julianagilloaiza soilgasprobesformonitoringtracegasmessengersofmicrobialactivity AT tillhmvolkmann soilgasprobesformonitoringtracegasmessengersofmicrobialactivity
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Soil gas probes for monitoring trace gas messengers of microbial activity

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